Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae)
The genus <i>Haematobosca</i> Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as <i>Haematobosca aberrans</i> Pont, Du...
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MDPI AG
2020-07-01
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| Series: | Insects |
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| author | Tanasak Changbunjong Jiraporn Ruangsittichai Gerard Duvallet Adrian C. Pont |
| author_facet | Tanasak Changbunjong Jiraporn Ruangsittichai Gerard Duvallet Adrian C. Pont |
| author_sort | Tanasak Changbunjong |
| collection | DOAJ |
| description | The genus <i>Haematobosca</i> Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as <i>Haematobosca aberrans</i> Pont, Duvallet & Changbunjong, 2020. In the present study, the mitochondrial cytochrome c oxidase I (COI) gene was used to confirm the morphological identification of <i>H. aberrans</i>. In addition, landmark-based geometric morphometrics was used to determine sexual dimorphism. The molecular analysis was conducted with 10 COI sequences. The results showed that all sequences were 100% identical. The sequence was not highly similar to reference sequences from GenBank and did not match any identified species from Barcode of Life Data Systems (BOLD). Phylogenetic analysis clearly differentiated this species from other species within the subfamily Stomoxyinae. For geometric morphometric analysis, a total of 16 wing pictures were analysed using the landmark-based approach. The results showed significant differences in wing shape between males and females, with a cross-validated classification score of 100%. The allometric analysis showed that wing shape has no correlation with size. Therefore, the COI gene is effective in species identification of <i>H. aberrans</i>, and geometric morphometrics is also effective in determining sexual dimorphism. |
| first_indexed | 2024-03-10T18:26:51Z |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:26:51Z |
| publishDate | 2020-07-01 |
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| series | Insects |
| spelling | doaj.art-8f7b9823c4b046a0a94b51c63be2fbb72023-11-20T06:58:55ZengMDPI AGInsects2075-44502020-07-0111745110.3390/insects11070451Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae)Tanasak Changbunjong0Jiraporn Ruangsittichai1Gerard Duvallet2Adrian C. Pont3Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, ThailandDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandUMR5175 CEFE, Centre d’Ecologie Fonctionnelle et Evolutive, Université Paul-Valéry, 34090 Montpellier, FranceOxford University Museum of Natural History, Parks Road, Oxford OX1 3PW, UKThe genus <i>Haematobosca</i> Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as <i>Haematobosca aberrans</i> Pont, Duvallet & Changbunjong, 2020. In the present study, the mitochondrial cytochrome c oxidase I (COI) gene was used to confirm the morphological identification of <i>H. aberrans</i>. In addition, landmark-based geometric morphometrics was used to determine sexual dimorphism. The molecular analysis was conducted with 10 COI sequences. The results showed that all sequences were 100% identical. The sequence was not highly similar to reference sequences from GenBank and did not match any identified species from Barcode of Life Data Systems (BOLD). Phylogenetic analysis clearly differentiated this species from other species within the subfamily Stomoxyinae. For geometric morphometric analysis, a total of 16 wing pictures were analysed using the landmark-based approach. The results showed significant differences in wing shape between males and females, with a cross-validated classification score of 100%. The allometric analysis showed that wing shape has no correlation with size. Therefore, the COI gene is effective in species identification of <i>H. aberrans</i>, and geometric morphometrics is also effective in determining sexual dimorphism.https://www.mdpi.com/2075-4450/11/7/451cytochrome c oxidase Igeometric morphometrics<i>Haematobosca</i>sexual dimorphismStomoxyinaeThailand |
| spellingShingle | Tanasak Changbunjong Jiraporn Ruangsittichai Gerard Duvallet Adrian C. Pont Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae) Insects cytochrome c oxidase I geometric morphometrics <i>Haematobosca</i> sexual dimorphism Stomoxyinae Thailand |
| title | Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae) |
| title_full | Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae) |
| title_fullStr | Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae) |
| title_full_unstemmed | Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae) |
| title_short | Molecular Identification and Geometric Morphometric Analysis of <i>Haematobosca</i> <i>aberrans</i> (Diptera: Muscidae) |
| title_sort | molecular identification and geometric morphometric analysis of i haematobosca i i aberrans i diptera muscidae |
| topic | cytochrome c oxidase I geometric morphometrics <i>Haematobosca</i> sexual dimorphism Stomoxyinae Thailand |
| url | https://www.mdpi.com/2075-4450/11/7/451 |
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