Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex
ABSTRACT Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorou...
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-021-00064-0 |
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| author | Ruben De Lange Slavomír Adamčík Katarína Adamčíkova Pieter Asselman Jan Borovička Lynn Delgat Felix Hampe Annemieke Verbeken |
| author_facet | Ruben De Lange Slavomír Adamčík Katarína Adamčíkova Pieter Asselman Jan Borovička Lynn Delgat Felix Hampe Annemieke Verbeken |
| author_sort | Ruben De Lange |
| collection | DOAJ |
| description | ABSTRACT Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences. |
| first_indexed | 2024-12-19T16:07:13Z |
| format | Article |
| id | doaj.art-8f7bcb7295cd4a16b71ebf45a1a5c825 |
| institution | Directory Open Access Journal |
| issn | 2210-6359 |
| language | English |
| last_indexed | 2024-12-19T16:07:13Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj.art-8f7bcb7295cd4a16b71ebf45a1a5c8252022-12-21T20:14:49ZengBMCIMA Fungus2210-63592021-08-0112113110.1186/s43008-021-00064-0Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complexRuben De Lange0Slavomír Adamčík1Katarína Adamčíkova2Pieter Asselman3Jan Borovička4Lynn Delgat5Felix Hampe6Annemieke Verbeken7Research Group Mycology, Department of Biology, Ghent UniversityInstitute of Botany, Plant Science and Biodiversity Center, Slovak Academy of SciencesInstitute of Forest Ecology Slovak Academy of SciencesResearch Group Mycology, Department of Biology, Ghent UniversityInstitute of Geology of the Czech Academy of SciencesResearch Group Mycology, Department of Biology, Ghent UniversityResearch Group Mycology, Department of Biology, Ghent UniversityResearch Group Mycology, Department of Biology, Ghent UniversityABSTRACT Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences.https://doi.org/10.1186/s43008-021-00064-0BasidiomycotaCoalescent species delimitationEctomycorrhizal fungiNew speciesPhylogenyRussulaceae |
| spellingShingle | Ruben De Lange Slavomír Adamčík Katarína Adamčíkova Pieter Asselman Jan Borovička Lynn Delgat Felix Hampe Annemieke Verbeken Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex IMA Fungus Basidiomycota Coalescent species delimitation Ectomycorrhizal fungi New species Phylogeny Russulaceae |
| title | Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex |
| title_full | Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex |
| title_fullStr | Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex |
| title_full_unstemmed | Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex |
| title_short | Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex |
| title_sort | enlightening the black and white species delimitation and unite species hypothesis testing in the russula albonigra species complex |
| topic | Basidiomycota Coalescent species delimitation Ectomycorrhizal fungi New species Phylogeny Russulaceae |
| url | https://doi.org/10.1186/s43008-021-00064-0 |
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