Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling
The treatment of osteoporotic fractures is a severe clinical issue, especially in cases where low support is provided, e.g., pelvis. New treatments aim to stimulate bone formation in compromised scenarios by using multifunctional biomaterials combined with biofabrication techniques to produce 3D str...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/18/3780 |
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| author | Priscila Melo Giorgia Montalbano Elena Boggio Casimiro Luca Gigliotti Chiara Dianzani Umberto Dianzani Chiara Vitale-Brovarone Sonia Fiorilli |
| author_facet | Priscila Melo Giorgia Montalbano Elena Boggio Casimiro Luca Gigliotti Chiara Dianzani Umberto Dianzani Chiara Vitale-Brovarone Sonia Fiorilli |
| author_sort | Priscila Melo |
| collection | DOAJ |
| description | The treatment of osteoporotic fractures is a severe clinical issue, especially in cases where low support is provided, e.g., pelvis. New treatments aim to stimulate bone formation in compromised scenarios by using multifunctional biomaterials combined with biofabrication techniques to produce 3D structures (scaffolds) that can support bone formation. Bone’s extracellular matrix (ECM) is mainly composed of type I collagen, making this material highly desirable in bone tissue engineering applications, and its bioactivity can be improved by incorporating specific biomolecules. In this work, type I collagen membranes were produced by electrospinning showing a fibre diameter below 200 nm. An optimized one-step strategy allowed to simultaneously crosslink the electrospun membranes and bind ICOS-Fc, a biomolecule able to reversibly inhibit osteoclast activity. The post-treatment did not alter the ECM-like nanostructure of the meshes and the physicochemical properties of collagen. UV-Vis and TGA analyses confirmed both crosslinking and grafting of ICOS-Fc onto the collagen fibres. The preservation of the biological activity of grafted ICOS-Fc was evidenced by the ability to affect the migratory activity of ICOSL-positive cells. The combination of ICOS-Fc with electrospun collagen represents a promising strategy to design multifunctional devices able to boost bone regeneration in osteoporotic fractures. |
| first_indexed | 2024-03-09T22:45:37Z |
| format | Article |
| id | doaj.art-8f7d448bef6446c5a38e6da36b5316bb |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T22:45:37Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-8f7d448bef6446c5a38e6da36b5316bb2023-11-23T18:29:46ZengMDPI AGPolymers2073-43602022-09-011418378010.3390/polym14183780Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone RemodellingPriscila Melo0Giorgia Montalbano1Elena Boggio2Casimiro Luca Gigliotti3Chiara Dianzani4Umberto Dianzani5Chiara Vitale-Brovarone6Sonia Fiorilli7Department of Applied Sciences and Technologies, Politecnico di Torino, Corso Duca Degli Abruzzi 24, 10129 Torino, ItalyDepartment of Applied Sciences and Technologies, Politecnico di Torino, Corso Duca Degli Abruzzi 24, 10129 Torino, ItalyNOVAICOS s.r.l.s, Via Amico Canobio 4/6, 28100 Novara, ItalyNOVAICOS s.r.l.s, Via Amico Canobio 4/6, 28100 Novara, ItalyDepartment of Drug Science and Technology, Università degli Studi di Torino, Via Pietro Giuria 9, 10125 Torino, ItalyDepartment of Health Sciences, Università del Piemonte Orientale, Via Solaroli 17, 28100 Novara, ItalyDepartment of Applied Sciences and Technologies, Politecnico di Torino, Corso Duca Degli Abruzzi 24, 10129 Torino, ItalyDepartment of Applied Sciences and Technologies, Politecnico di Torino, Corso Duca Degli Abruzzi 24, 10129 Torino, ItalyThe treatment of osteoporotic fractures is a severe clinical issue, especially in cases where low support is provided, e.g., pelvis. New treatments aim to stimulate bone formation in compromised scenarios by using multifunctional biomaterials combined with biofabrication techniques to produce 3D structures (scaffolds) that can support bone formation. Bone’s extracellular matrix (ECM) is mainly composed of type I collagen, making this material highly desirable in bone tissue engineering applications, and its bioactivity can be improved by incorporating specific biomolecules. In this work, type I collagen membranes were produced by electrospinning showing a fibre diameter below 200 nm. An optimized one-step strategy allowed to simultaneously crosslink the electrospun membranes and bind ICOS-Fc, a biomolecule able to reversibly inhibit osteoclast activity. The post-treatment did not alter the ECM-like nanostructure of the meshes and the physicochemical properties of collagen. UV-Vis and TGA analyses confirmed both crosslinking and grafting of ICOS-Fc onto the collagen fibres. The preservation of the biological activity of grafted ICOS-Fc was evidenced by the ability to affect the migratory activity of ICOSL-positive cells. The combination of ICOS-Fc with electrospun collagen represents a promising strategy to design multifunctional devices able to boost bone regeneration in osteoporotic fractures.https://www.mdpi.com/2073-4360/14/18/3780type I collagenelectrospinningICOS-Fccell migrationbone remodellingosteoporosis |
| spellingShingle | Priscila Melo Giorgia Montalbano Elena Boggio Casimiro Luca Gigliotti Chiara Dianzani Umberto Dianzani Chiara Vitale-Brovarone Sonia Fiorilli Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling Polymers type I collagen electrospinning ICOS-Fc cell migration bone remodelling osteoporosis |
| title | Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling |
| title_full | Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling |
| title_fullStr | Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling |
| title_full_unstemmed | Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling |
| title_short | Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling |
| title_sort | electrospun collagen scaffold bio functionalized with recombinant icos fc an advanced approach to promote bone remodelling |
| topic | type I collagen electrospinning ICOS-Fc cell migration bone remodelling osteoporosis |
| url | https://www.mdpi.com/2073-4360/14/18/3780 |
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