Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L.
<i>Artemisia vulgaris</i> is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of <i>Artemisia vulgaris</...
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2279 |
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| author | Nameirakpam Bunindro Singh Moirangthem Lakshmipriyari Devi Thokchom Biona Nanaocha Sharma Sudripta Das Jharna Chakravorty Pulok Kumar Mukherjee Yallappa Rajashekar |
| author_facet | Nameirakpam Bunindro Singh Moirangthem Lakshmipriyari Devi Thokchom Biona Nanaocha Sharma Sudripta Das Jharna Chakravorty Pulok Kumar Mukherjee Yallappa Rajashekar |
| author_sort | Nameirakpam Bunindro Singh |
| collection | DOAJ |
| description | <i>Artemisia vulgaris</i> is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of <i>Artemisia vulgaris</i> essential oil (AVEO) from the fresh leaves of <i>A. vulgaris</i> grown in Manipur. The AVEO isolated by hydro-distillation from <i>A. vulgaris</i> were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component in the leaf volatiles comes to the terms of monoterpenes. The AVEO exhibits antimicrobial activities against fungal pathogens such as <i>Sclerotium oryzae</i> (ITCC 4107) and <i>Fusarium oxysporum</i> (MTCC 9913) and bacterial cultures such as <i>Bacillus cereus</i> (ATCC 13061) and <i>Staphylococcus aureus</i> (ATCC 25923). The percent inhibition of AVEO against the <i>S. oryzae</i> and <i>F. oxysporum</i> was found up to 50.3% and 33.13%, respectively. The MIC and MBC of the essential oil tested for <i>B. cereus</i> and <i>S. aureus</i> were found to be (0.3%, 0.63%) and (0.63%, 2.5%), respectively. Finally, the results revealed that the AVEO characterized by the hydro-distillation and SPME extraction yielded the same chemical profile and showed potent antimicrobial activities. Further research into <i>A. vulgaris’s</i> antibacterial properties can be performed in order to use it as a source for natural antimicrobial medications. |
| first_indexed | 2024-03-11T07:16:47Z |
| format | Article |
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| spelling | doaj.art-8f7dd49bebc54829b61cb0ab966db00b2023-11-17T08:14:29ZengMDPI AGMolecules1420-30492023-02-01285227910.3390/molecules28052279Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L.Nameirakpam Bunindro Singh0Moirangthem Lakshmipriyari Devi1Thokchom Biona2Nanaocha Sharma3Sudripta Das4Jharna Chakravorty5Pulok Kumar Mukherjee6Yallappa Rajashekar7Insect Resources Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, IndiaPlant Molecular Genetics and Genomics Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, IndiaInsect Resources Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, IndiaInsect Resources Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, IndiaPlant Molecular Genetics and Genomics Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, IndiaDepartment of Zoology, Rajiv Gandhi University, Itanagar 781014, IndiaInsect Resources Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, IndiaInsect Resources Laboratory, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal 795001, India<i>Artemisia vulgaris</i> is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of <i>Artemisia vulgaris</i> essential oil (AVEO) from the fresh leaves of <i>A. vulgaris</i> grown in Manipur. The AVEO isolated by hydro-distillation from <i>A. vulgaris</i> were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component in the leaf volatiles comes to the terms of monoterpenes. The AVEO exhibits antimicrobial activities against fungal pathogens such as <i>Sclerotium oryzae</i> (ITCC 4107) and <i>Fusarium oxysporum</i> (MTCC 9913) and bacterial cultures such as <i>Bacillus cereus</i> (ATCC 13061) and <i>Staphylococcus aureus</i> (ATCC 25923). The percent inhibition of AVEO against the <i>S. oryzae</i> and <i>F. oxysporum</i> was found up to 50.3% and 33.13%, respectively. The MIC and MBC of the essential oil tested for <i>B. cereus</i> and <i>S. aureus</i> were found to be (0.3%, 0.63%) and (0.63%, 2.5%), respectively. Finally, the results revealed that the AVEO characterized by the hydro-distillation and SPME extraction yielded the same chemical profile and showed potent antimicrobial activities. Further research into <i>A. vulgaris’s</i> antibacterial properties can be performed in order to use it as a source for natural antimicrobial medications.https://www.mdpi.com/1420-3049/28/5/2279<i>Artemisia vulgaris</i>essential oilgas chromatography/mass spectrometrysolid-phase microextractioneucalyptolantimicrobial |
| spellingShingle | Nameirakpam Bunindro Singh Moirangthem Lakshmipriyari Devi Thokchom Biona Nanaocha Sharma Sudripta Das Jharna Chakravorty Pulok Kumar Mukherjee Yallappa Rajashekar Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L. Molecules <i>Artemisia vulgaris</i> essential oil gas chromatography/mass spectrometry solid-phase microextraction eucalyptol antimicrobial |
| title | Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L. |
| title_full | Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L. |
| title_fullStr | Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L. |
| title_full_unstemmed | Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L. |
| title_short | Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of <i>Artemisia vulgaris</i> L. |
| title_sort | phytochemical composition and antimicrobial activity of essential oil from the leaves of i artemisia vulgaris i l |
| topic | <i>Artemisia vulgaris</i> essential oil gas chromatography/mass spectrometry solid-phase microextraction eucalyptol antimicrobial |
| url | https://www.mdpi.com/1420-3049/28/5/2279 |
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