A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type
Summary: After fertilization, microtubule (MT) sperm asters undergo long-range migration to accurately position pronuclei. Due to the large sizes of zygotes, the forces driving aster migration are considered to be from pulling on the astral MTs by dynein, with no significant contribution from pushin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-10-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472031202X |
| _version_ | 1818966984033828864 |
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| author | Johnathan L. Meaders Salvador N. de Matos David R. Burgess |
| author_facet | Johnathan L. Meaders Salvador N. de Matos David R. Burgess |
| author_sort | Johnathan L. Meaders |
| collection | DOAJ |
| description | Summary: After fertilization, microtubule (MT) sperm asters undergo long-range migration to accurately position pronuclei. Due to the large sizes of zygotes, the forces driving aster migration are considered to be from pulling on the astral MTs by dynein, with no significant contribution from pushing forces. Here, we re-investigate the forces responsible for sperm aster centration in sea urchin zygotes. Our quantifications of aster geometry and MT density preclude a pulling mechanism. Manipulation of aster radial lengths and growth rates, combined with quantitative tracking of aster migration dynamics, indicates that aster migration is equal to the length of rear aster radii, supporting a pushing model for centration. We find that dynein inhibition causes an increase in aster migration rates. Finally, ablation of rear astral MTs halts migration, whereas front and side ablations do not. Collectively, our data indicate that a pushing mechanism can drive the migration of asters in a large cell type. |
| first_indexed | 2024-12-20T13:41:35Z |
| format | Article |
| id | doaj.art-8f7ec094d291405dbac00ef0c57f151e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-20T13:41:35Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-8f7ec094d291405dbac00ef0c57f151e2022-12-21T19:38:47ZengElsevierCell Reports2211-12472020-10-01331108213A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell TypeJohnathan L. Meaders0Salvador N. de Matos1David R. Burgess2Department of Biology, Boston College, Chestnut Hill, MA 02467, USA; Marine Biological Laboratory, Woods Hole, MA 02543, USADepartment of Biology, Boston College, Chestnut Hill, MA 02467, USADepartment of Biology, Boston College, Chestnut Hill, MA 02467, USA; Marine Biological Laboratory, Woods Hole, MA 02543, USA; Corresponding authorSummary: After fertilization, microtubule (MT) sperm asters undergo long-range migration to accurately position pronuclei. Due to the large sizes of zygotes, the forces driving aster migration are considered to be from pulling on the astral MTs by dynein, with no significant contribution from pushing forces. Here, we re-investigate the forces responsible for sperm aster centration in sea urchin zygotes. Our quantifications of aster geometry and MT density preclude a pulling mechanism. Manipulation of aster radial lengths and growth rates, combined with quantitative tracking of aster migration dynamics, indicates that aster migration is equal to the length of rear aster radii, supporting a pushing model for centration. We find that dynein inhibition causes an increase in aster migration rates. Finally, ablation of rear astral MTs halts migration, whereas front and side ablations do not. Collectively, our data indicate that a pushing mechanism can drive the migration of asters in a large cell type.http://www.sciencedirect.com/science/article/pii/S221112472031202Xdyneinastermicrotubulecentrosomepronucleusfertilization |
| spellingShingle | Johnathan L. Meaders Salvador N. de Matos David R. Burgess A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type Cell Reports dynein aster microtubule centrosome pronucleus fertilization |
| title | A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type |
| title_full | A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type |
| title_fullStr | A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type |
| title_full_unstemmed | A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type |
| title_short | A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type |
| title_sort | pushing mechanism for microtubule aster positioning in a large cell type |
| topic | dynein aster microtubule centrosome pronucleus fertilization |
| url | http://www.sciencedirect.com/science/article/pii/S221112472031202X |
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