Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion
Three white rot fungi (Lenzites betulinus, Trametes orientalis, and Trametes velutina) as well as their respective paired cultures were used to pretreat Populus tomentosa for enhanced lignocellulosic degradation and enzymatic hydrolysis. Hemicellulose and cellulose were slightly degraded, while a ma...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-05-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_3968_Wang_Pretreatment_White_Rot_Fungi |
| _version_ | 1819031368047263744 |
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| author | Wei Wang Tongqi Yuan Baokai Cui |
| author_facet | Wei Wang Tongqi Yuan Baokai Cui |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Three white rot fungi (Lenzites betulinus, Trametes orientalis, and Trametes velutina) as well as their respective paired cultures were used to pretreat Populus tomentosa for enhanced lignocellulosic degradation and enzymatic hydrolysis. Hemicellulose and cellulose were slightly degraded, while a maximum lignin degradation of 58% was caused by T. velutina during the 12-week cultivation. After the pretreated samples were subjected to enzymatic hydrolysis for 96 h, the reducing sugar released by T. orientalis at week 12 was as high as 41%, which was in line with the lignin loss at 2.2 times the control sample. Overall, the monocultures of white-rot fungi exhibited better degradation and saccharification of woody biomass than their co-culture. This can be attributed to the partial removal of lignin and hemicellulose, with an associated increase of cellulose accessibility to enzymes. |
| first_indexed | 2024-12-21T06:44:56Z |
| format | Article |
| id | doaj.art-8f7ed8a69ee946fb8d4372f4ca8d8256 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-21T06:44:56Z |
| publishDate | 2014-05-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-8f7ed8a69ee946fb8d4372f4ca8d82562022-12-21T19:12:37ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-05-01933968397610.15376/biores.9.3.3968-3976Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic DigestionWei Wang0Tongqi Yuan1Baokai Cui2Institute of Microbiology, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, ChinaInstitute of Microbiology, Beijing Forestry University, Beijing 100083, ChinaThree white rot fungi (Lenzites betulinus, Trametes orientalis, and Trametes velutina) as well as their respective paired cultures were used to pretreat Populus tomentosa for enhanced lignocellulosic degradation and enzymatic hydrolysis. Hemicellulose and cellulose were slightly degraded, while a maximum lignin degradation of 58% was caused by T. velutina during the 12-week cultivation. After the pretreated samples were subjected to enzymatic hydrolysis for 96 h, the reducing sugar released by T. orientalis at week 12 was as high as 41%, which was in line with the lignin loss at 2.2 times the control sample. Overall, the monocultures of white-rot fungi exhibited better degradation and saccharification of woody biomass than their co-culture. This can be attributed to the partial removal of lignin and hemicellulose, with an associated increase of cellulose accessibility to enzymes.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_3968_Wang_Pretreatment_White_Rot_FungiCo-cultureBiological pretreatmentEnzymatic hydrolysisEthanolWoody biomass |
| spellingShingle | Wei Wang Tongqi Yuan Baokai Cui Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion BioResources Co-culture Biological pretreatment Enzymatic hydrolysis Ethanol Woody biomass |
| title | Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion |
| title_full | Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion |
| title_fullStr | Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion |
| title_full_unstemmed | Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion |
| title_short | Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion |
| title_sort | biological pretreatment with white rot fungi and their co culture to overcome lignocellulosic recalcitrance for improved enzymatic digestion |
| topic | Co-culture Biological pretreatment Enzymatic hydrolysis Ethanol Woody biomass |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_3968_Wang_Pretreatment_White_Rot_Fungi |
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