Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding
IntroductionNon-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different...
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| Format: | Article |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1205703/full |
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| author | Olga Horakova Gabriella Sistilli Gabriella Sistilli Veronika Kalendova Veronika Kalendova Kristina Bardova Marko Mitrovic Marko Mitrovic Tomas Cajka Ilaria Irodenko Petra Janovska Karoline Lackner Jan Kopecky Martin Rossmeisl |
| author_facet | Olga Horakova Gabriella Sistilli Gabriella Sistilli Veronika Kalendova Veronika Kalendova Kristina Bardova Marko Mitrovic Marko Mitrovic Tomas Cajka Ilaria Irodenko Petra Janovska Karoline Lackner Jan Kopecky Martin Rossmeisl |
| author_sort | Olga Horakova |
| collection | DOAJ |
| description | IntroductionNon-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different C57BL/6 substrains, we examined how thermoneutrality affects NAFLD progression in C57BL/6N mice.MethodsMale mice were fed standard or high-fat diet for 24 weeks and housed under standard (22°C) or thermoneutral (30°C) conditions.ResultsHigh-fat feeding promoted weight gain and hepatic steatosis, but the effect of thermoneutral environment was not evident. Liver expression of inflammatory markers was increased, with a modest and inconsistent effect of thermoneutral housing; however, histological scores of inflammation and fibrosis were generally low (<1.0), regardless of ambient temperature. In standard diet-fed mice, thermoneutrality increased weight gain, adiposity, and hepatic steatosis, accompanied by elevated de novo lipogenesis and changes in liver metabolome characterized by complex decreases in phospholipids and metabolites involved in urea cycle and oxidative stress defense.ConclusionThermoneutrality appears to promote NAFLD-associated phenotypes depending on the C57BL/6 substrain and/or the amount of dietary fat. |
| first_indexed | 2024-03-13T00:06:55Z |
| format | Article |
| id | doaj.art-8f805a1becbe4000a52309d7f53c4d18 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-13T00:06:55Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-8f805a1becbe4000a52309d7f53c4d182023-07-13T00:00:22ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-07-011410.3389/fendo.2023.12057031205703Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feedingOlga Horakova0Gabriella Sistilli1Gabriella Sistilli2Veronika Kalendova3Veronika Kalendova4Kristina Bardova5Marko Mitrovic6Marko Mitrovic7Tomas Cajka8Ilaria Irodenko9Petra Janovska10Karoline Lackner11Jan Kopecky12Martin Rossmeisl13Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaFaculty of Science, Charles University, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaFaculty of Science, Charles University, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaFirst Faculty of Medicine, Charles University, Prague, CzechiaLaboratory of Translational Metabolism, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaInstitute of Pathology, Medical University of Graz, Graz, AustriaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaLaboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, CzechiaIntroductionNon-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different C57BL/6 substrains, we examined how thermoneutrality affects NAFLD progression in C57BL/6N mice.MethodsMale mice were fed standard or high-fat diet for 24 weeks and housed under standard (22°C) or thermoneutral (30°C) conditions.ResultsHigh-fat feeding promoted weight gain and hepatic steatosis, but the effect of thermoneutral environment was not evident. Liver expression of inflammatory markers was increased, with a modest and inconsistent effect of thermoneutral housing; however, histological scores of inflammation and fibrosis were generally low (<1.0), regardless of ambient temperature. In standard diet-fed mice, thermoneutrality increased weight gain, adiposity, and hepatic steatosis, accompanied by elevated de novo lipogenesis and changes in liver metabolome characterized by complex decreases in phospholipids and metabolites involved in urea cycle and oxidative stress defense.ConclusionThermoneutrality appears to promote NAFLD-associated phenotypes depending on the C57BL/6 substrain and/or the amount of dietary fat.https://www.frontiersin.org/articles/10.3389/fendo.2023.1205703/fullnon-alcoholic fatty liver diseaseliver steatosisNASHC57BL/6N miceobesitythermoneutrality |
| spellingShingle | Olga Horakova Gabriella Sistilli Gabriella Sistilli Veronika Kalendova Veronika Kalendova Kristina Bardova Marko Mitrovic Marko Mitrovic Tomas Cajka Ilaria Irodenko Petra Janovska Karoline Lackner Jan Kopecky Martin Rossmeisl Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding Frontiers in Endocrinology non-alcoholic fatty liver disease liver steatosis NASH C57BL/6N mice obesity thermoneutrality |
| title | Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding |
| title_full | Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding |
| title_fullStr | Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding |
| title_full_unstemmed | Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding |
| title_short | Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding |
| title_sort | thermoneutral housing promotes hepatic steatosis in standard diet fed c57bl 6n mice with a less pronounced effect on nafld progression upon high fat feeding |
| topic | non-alcoholic fatty liver disease liver steatosis NASH C57BL/6N mice obesity thermoneutrality |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1205703/full |
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