Cell-free synthetic biology: Engineering in an open world
Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides simpler and faster engineering solutions with an unprecedented freedom of design in an open environm...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2017-03-01
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| Series: | Synthetic and Systems Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X1730008X |
| _version_ | 1797203309047578624 |
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| author | Yuan Lu |
| author_facet | Yuan Lu |
| author_sort | Yuan Lu |
| collection | DOAJ |
| description | Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides simpler and faster engineering solutions with an unprecedented freedom of design in an open environment than cell system. This review focuses on recent developments of cell-free synthetic biology on biological engineering fields at molecular and cellular levels, including protein engineering, metabolic engineering, and artificial cell engineering. In cell-free protein engineering, the direct control of reaction conditions in cell-free system allows for easy synthesis of complex proteins, toxic proteins, membrane proteins, and novel proteins with unnatural amino acids. Cell-free systems offer the ability to design metabolic pathways towards the production of desired products. Buildup of artificial cells based on cell-free systems will improve our understanding of life and use them for environmental and biomedical applications. |
| first_indexed | 2024-04-24T08:17:17Z |
| format | Article |
| id | doaj.art-8f82359cdf7e48998688fd7dbd72054d |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:17:17Z |
| publishDate | 2017-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-8f82359cdf7e48998688fd7dbd72054d2024-04-17T03:22:07ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2017-03-0121232710.1016/j.synbio.2017.02.003Cell-free synthetic biology: Engineering in an open worldYuan Lu0Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaCell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides simpler and faster engineering solutions with an unprecedented freedom of design in an open environment than cell system. This review focuses on recent developments of cell-free synthetic biology on biological engineering fields at molecular and cellular levels, including protein engineering, metabolic engineering, and artificial cell engineering. In cell-free protein engineering, the direct control of reaction conditions in cell-free system allows for easy synthesis of complex proteins, toxic proteins, membrane proteins, and novel proteins with unnatural amino acids. Cell-free systems offer the ability to design metabolic pathways towards the production of desired products. Buildup of artificial cells based on cell-free systems will improve our understanding of life and use them for environmental and biomedical applications.http://www.sciencedirect.com/science/article/pii/S2405805X1730008XCell-free synthetic biologyCell-free protein synthesisProtein engineeringMetabolic engineeringArtificial cellUnnatural amino acids |
| spellingShingle | Yuan Lu Cell-free synthetic biology: Engineering in an open world Synthetic and Systems Biotechnology Cell-free synthetic biology Cell-free protein synthesis Protein engineering Metabolic engineering Artificial cell Unnatural amino acids |
| title | Cell-free synthetic biology: Engineering in an open world |
| title_full | Cell-free synthetic biology: Engineering in an open world |
| title_fullStr | Cell-free synthetic biology: Engineering in an open world |
| title_full_unstemmed | Cell-free synthetic biology: Engineering in an open world |
| title_short | Cell-free synthetic biology: Engineering in an open world |
| title_sort | cell free synthetic biology engineering in an open world |
| topic | Cell-free synthetic biology Cell-free protein synthesis Protein engineering Metabolic engineering Artificial cell Unnatural amino acids |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X1730008X |
| work_keys_str_mv | AT yuanlu cellfreesyntheticbiologyengineeringinanopenworld |