Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-03-01
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| Series: | Iranian Journal of Public Health |
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| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/9389 |
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| author | Solmaz OHADIAN MOGHADAM Mohammad Reza POURMAND Masoumeh DOURAGHI Samira SABZI Parisa GHAFFARI |
| author_facet | Solmaz OHADIAN MOGHADAM Mohammad Reza POURMAND Masoumeh DOURAGHI Samira SABZI Parisa GHAFFARI |
| author_sort | Solmaz OHADIAN MOGHADAM |
| collection | DOAJ |
| description | Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed to define the genetic relatedness of MRSA strains isolated from burn patients by analyzing the large fragments of DNA.
Methods: In this cross-sectional study, 126 pus/wound swabs from skin and soft tissue infections (SSTIs) were collected from inpatients of Shahid Motahari Burn Center (Tehran, Iran) in 2013. Then, molecular typing of MRSA was achieved by Pulsed-Field Gel Electrophoresis (PFGE).
Results: The PFGE analysis of MRSA indicated 31 single types and 5 common types. There was a significant diversity in the chromosomal digestion pattern of the MRSA strains explained by the acquisition of MRSA from various sources.
Conclusion: The permanent import of novel types of MRSA strains despite the rigorous infection control measures carried out within the center. The importance of PFGE in understanding the epidemiology of MRSA may serve as a basis for the development of rational control strategies. |
| first_indexed | 2024-12-19T16:10:00Z |
| format | Article |
| id | doaj.art-8f8263767c5048caa0e275464978f8d6 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-19T16:10:00Z |
| publishDate | 2017-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-8f8263767c5048caa0e275464978f8d62022-12-21T20:14:46ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932017-03-01463Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA StrainsSolmaz OHADIAN MOGHADAM0Mohammad Reza POURMAND1Masoumeh DOURAGHI2Samira SABZI3Parisa GHAFFARI4Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, IranDept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBiotechnology Research Center, Tehran University of Medical Sciences, Tehran, IranBackground: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed to define the genetic relatedness of MRSA strains isolated from burn patients by analyzing the large fragments of DNA. Methods: In this cross-sectional study, 126 pus/wound swabs from skin and soft tissue infections (SSTIs) were collected from inpatients of Shahid Motahari Burn Center (Tehran, Iran) in 2013. Then, molecular typing of MRSA was achieved by Pulsed-Field Gel Electrophoresis (PFGE). Results: The PFGE analysis of MRSA indicated 31 single types and 5 common types. There was a significant diversity in the chromosomal digestion pattern of the MRSA strains explained by the acquisition of MRSA from various sources. Conclusion: The permanent import of novel types of MRSA strains despite the rigorous infection control measures carried out within the center. The importance of PFGE in understanding the epidemiology of MRSA may serve as a basis for the development of rational control strategies.https://ijph.tums.ac.ir/index.php/ijph/article/view/9389Molecular typingMethicillin-resistant staphylococcus aureusPulsed-field gel electrophoresisBurn units |
| spellingShingle | Solmaz OHADIAN MOGHADAM Mohammad Reza POURMAND Masoumeh DOURAGHI Samira SABZI Parisa GHAFFARI Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains Iranian Journal of Public Health Molecular typing Methicillin-resistant staphylococcus aureus Pulsed-field gel electrophoresis Burn units |
| title | Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains |
| title_full | Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains |
| title_fullStr | Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains |
| title_full_unstemmed | Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains |
| title_short | Utilization of PFGE as a Powerful Discriminative Tool for the Investigation of Genetic Diversity among MRSA Strains |
| title_sort | utilization of pfge as a powerful discriminative tool for the investigation of genetic diversity among mrsa strains |
| topic | Molecular typing Methicillin-resistant staphylococcus aureus Pulsed-field gel electrophoresis Burn units |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/9389 |
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