Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages

This study was conducted to screen the lactic acid bacteria (LAB) with admirable technological properties and high protease activity from traditional Chinese fermented sausages, and to investigate the effects of LAB on the antioxidant activity of peptides of fermented sausages. Seven strains were ob...

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Main Authors: Chen-Chen Cao, Mei-Qin Feng, Jian Sun, Xing-Lian Xu, Guang-Hong Zhou
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:CyTA - Journal of Food
Subjects:
Online Access:http://dx.doi.org/10.1080/19476337.2019.1583687
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author Chen-Chen Cao
Mei-Qin Feng
Jian Sun
Xing-Lian Xu
Guang-Hong Zhou
author_facet Chen-Chen Cao
Mei-Qin Feng
Jian Sun
Xing-Lian Xu
Guang-Hong Zhou
author_sort Chen-Chen Cao
collection DOAJ
description This study was conducted to screen the lactic acid bacteria (LAB) with admirable technological properties and high protease activity from traditional Chinese fermented sausages, and to investigate the effects of LAB on the antioxidant activity of peptides of fermented sausages. Seven strains were obtained from 133 LAB isolates. They complied with the criteria of meat starter cultures with high proteolytic activity. These strains were analyzed by API and sequence analysis of 16S rRNA to identify the five strains of Lactobacillus plantarum, one strain of L. fermentum, and one strain of L. pentosus. Furthermore, L. plantarum CD101 was inoculated with fermented sausages. LC-MS/MS was used to analyze the peptide composition. Compared with control group, using L. plantarum CD101 as starter cultures (Group L) led to a significant increase (P < 0.05) in antioxidant activity. The relative content of most peptides in Group L samples was largely higher than that in control samples.
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spelling doaj.art-8f83914777924d52b4b40be9eb0fada62022-12-22T03:54:24ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452019-01-0117134735410.1080/19476337.2019.15836871583687Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausagesChen-Chen Cao0Mei-Qin Feng1Jian Sun2Xing-Lian Xu3Guang-Hong Zhou4Nanjing Agricultural UniversityJinling Institute of TechnologyNanjing Agricultural UniversityNanjing Agricultural UniversityNanjing Agricultural UniversityThis study was conducted to screen the lactic acid bacteria (LAB) with admirable technological properties and high protease activity from traditional Chinese fermented sausages, and to investigate the effects of LAB on the antioxidant activity of peptides of fermented sausages. Seven strains were obtained from 133 LAB isolates. They complied with the criteria of meat starter cultures with high proteolytic activity. These strains were analyzed by API and sequence analysis of 16S rRNA to identify the five strains of Lactobacillus plantarum, one strain of L. fermentum, and one strain of L. pentosus. Furthermore, L. plantarum CD101 was inoculated with fermented sausages. LC-MS/MS was used to analyze the peptide composition. Compared with control group, using L. plantarum CD101 as starter cultures (Group L) led to a significant increase (P < 0.05) in antioxidant activity. The relative content of most peptides in Group L samples was largely higher than that in control samples.http://dx.doi.org/10.1080/19476337.2019.1583687lactic acid bacteriafermented sausagestechnological propertiesproteolytic activityantioxidant activity
spellingShingle Chen-Chen Cao
Mei-Qin Feng
Jian Sun
Xing-Lian Xu
Guang-Hong Zhou
Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
CyTA - Journal of Food
lactic acid bacteria
fermented sausages
technological properties
proteolytic activity
antioxidant activity
title Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
title_full Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
title_fullStr Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
title_full_unstemmed Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
title_short Screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
title_sort screening of lactic acid bacteria with high protease activity from fermented sausages and antioxidant activity assessment of its fermented sausages
topic lactic acid bacteria
fermented sausages
technological properties
proteolytic activity
antioxidant activity
url http://dx.doi.org/10.1080/19476337.2019.1583687
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