The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats
Introduction: Dendrobium is the famous Chinese medicine. Dendrobium nobile Lindl. alkaloids (DNLA) is the active ingredient and has been shown to have anti-aging, anti-Alzheimer's disease, and neuroprotective properties. This study examined effect of DNLA on chronic unpredictable mild stress (C...
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| Format: | Article |
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Elsevier
2023-06-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142523000362 |
| _version_ | 1797836896292831232 |
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| author | Ting-Wang Xiong Bo Liu Qin Wu Yun-Yan Xu Jie Liu Jing-Shan Shi |
| author_facet | Ting-Wang Xiong Bo Liu Qin Wu Yun-Yan Xu Jie Liu Jing-Shan Shi |
| author_sort | Ting-Wang Xiong |
| collection | DOAJ |
| description | Introduction: Dendrobium is the famous Chinese medicine. Dendrobium nobile Lindl. alkaloids (DNLA) is the active ingredient and has been shown to have anti-aging, anti-Alzheimer's disease, and neuroprotective properties. This study examined effect of DNLA on chronic unpredictable mild stress (CUMS)-induced anxiety and depression in Fawn-Hooded (FH/Wjd) rats, a depression model in comorbid of alcoholism. Methods: FH/Wjd rats were subjected to CUMS for 49 days, followed by treatment with DNLA (20 mg/kg/day, po) for 35 days. The anxiety/depression behaviors, Nissl bodies, neurotransmitters and hypothalamic-pituitary-adrenal axis were examined. RNA-Seq analysis was performed to explore the protection mechanisms. Results: The CUMS-induced anxiety/depressive-like behaviors were ameliorated by DNLA in the elevated-plus-maze test, open-field test, and forced swimming test. The CUMS reduced Nissl bodies in the hippocampal CA2 region and cortex was alleviated by DNLA. CUMS disrupted 5-hydroxytryptamine, dopamine and gamma-aminobutyric acid in the brain, which were recovered by DNLA. Increases in serum adrenocorticotropic hormone and corticosterone levels were prevented by DNLA. RNA-Seq revealed CUMS-induced 1607 differentially expressed genes, which were ameliorated with DNLA treatment. KEGG showed the enrichment of Ribosome, Rap1 signaling, Tight junction, and RNA transport pathways. Discussion: FH/Wjd rats were susceptible to CUMS-induced anxiety and depression. DNLA ameliorated CUMS-induced anxiety/depression behaviors and neuronal damage. Multiple mechanisms were involved in the beneficial effects of DNLA, including neurotransmitters, the hypothalamic-pituitary-adrenal axis, and the hippocampal gene expressions. |
| first_indexed | 2024-04-09T15:16:13Z |
| format | Article |
| id | doaj.art-8f87629e2d1c4a5184db652828be573f |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-04-09T15:16:13Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-8f87629e2d1c4a5184db652828be573f2023-04-29T14:59:16ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252023-06-017100250The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in ratsTing-Wang Xiong0Bo Liu1Qin Wu2Yun-Yan Xu3Jie Liu4Jing-Shan Shi5Key Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, China; Zunyi Medical and Pharmaceutical College, Zunyi, ChinaKey Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, ChinaKey Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, ChinaKey Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, ChinaKey Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, China; Corresponding authors.Key Lab for Basic Pharmacology of Ministry of Education, Zunyi Medical University, Zunyi, China; Corresponding authors.Introduction: Dendrobium is the famous Chinese medicine. Dendrobium nobile Lindl. alkaloids (DNLA) is the active ingredient and has been shown to have anti-aging, anti-Alzheimer's disease, and neuroprotective properties. This study examined effect of DNLA on chronic unpredictable mild stress (CUMS)-induced anxiety and depression in Fawn-Hooded (FH/Wjd) rats, a depression model in comorbid of alcoholism. Methods: FH/Wjd rats were subjected to CUMS for 49 days, followed by treatment with DNLA (20 mg/kg/day, po) for 35 days. The anxiety/depression behaviors, Nissl bodies, neurotransmitters and hypothalamic-pituitary-adrenal axis were examined. RNA-Seq analysis was performed to explore the protection mechanisms. Results: The CUMS-induced anxiety/depressive-like behaviors were ameliorated by DNLA in the elevated-plus-maze test, open-field test, and forced swimming test. The CUMS reduced Nissl bodies in the hippocampal CA2 region and cortex was alleviated by DNLA. CUMS disrupted 5-hydroxytryptamine, dopamine and gamma-aminobutyric acid in the brain, which were recovered by DNLA. Increases in serum adrenocorticotropic hormone and corticosterone levels were prevented by DNLA. RNA-Seq revealed CUMS-induced 1607 differentially expressed genes, which were ameliorated with DNLA treatment. KEGG showed the enrichment of Ribosome, Rap1 signaling, Tight junction, and RNA transport pathways. Discussion: FH/Wjd rats were susceptible to CUMS-induced anxiety and depression. DNLA ameliorated CUMS-induced anxiety/depression behaviors and neuronal damage. Multiple mechanisms were involved in the beneficial effects of DNLA, including neurotransmitters, the hypothalamic-pituitary-adrenal axis, and the hippocampal gene expressions.http://www.sciencedirect.com/science/article/pii/S2667142523000362Dendrobium nobile Lindl. alkaloids (DNLA)Chronic unpredictable mild stress (CUMS)Fawn-Hooded (FH/Wjd) ratsAnxiety and depressionNeurotransmittersRNA-Seq analysis |
| spellingShingle | Ting-Wang Xiong Bo Liu Qin Wu Yun-Yan Xu Jie Liu Jing-Shan Shi The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats Pharmacological Research - Modern Chinese Medicine Dendrobium nobile Lindl. alkaloids (DNLA) Chronic unpredictable mild stress (CUMS) Fawn-Hooded (FH/Wjd) rats Anxiety and depression Neurotransmitters RNA-Seq analysis |
| title | The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats |
| title_full | The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats |
| title_fullStr | The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats |
| title_full_unstemmed | The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats |
| title_short | The protective effects of Dendrobium nobile Lindl. alkaloids (DNLA) against CUMS-induced anxiety/depression and hippocampal transcriptome changes in rats |
| title_sort | protective effects of dendrobium nobile lindl alkaloids dnla against cums induced anxiety depression and hippocampal transcriptome changes in rats |
| topic | Dendrobium nobile Lindl. alkaloids (DNLA) Chronic unpredictable mild stress (CUMS) Fawn-Hooded (FH/Wjd) rats Anxiety and depression Neurotransmitters RNA-Seq analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2667142523000362 |
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