Methylation Analysis of CpG Islands in Pineapple <i>SERK1</i> Promoter
Somatic embryogenesis (SE) is a more rapid and controllable method for plant propagation than traditional breeding methods. However, it often suffers from limited efficiency. <i>SERK1</i> promotes SE in several plants, including pineapple (<i>Ananas comosus</i> L.). We invest...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
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| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/11/4/425 |
| Summary: | Somatic embryogenesis (SE) is a more rapid and controllable method for plant propagation than traditional breeding methods. However, it often suffers from limited efficiency. <i>SERK1</i> promotes SE in several plants, including pineapple (<i>Ananas comosus</i> L.). We investigate the embryonic cell-specific transcriptional regulation of <i>AcSERK1</i> by methylation analysis of CpG islands in <i>AcSERK1</i> regulatory sequences. This revealed differences in the methylation status of CpG islands between embryonic callus and non-embryonic callus; the methylation inhibitor 5-azaC increased <i>AcSERK1</i> expression and also accelerated SE. These findings indicate that the expression of <i>AcSERK1</i> is regulated epigenetically. This study lays the foundation for further analysis of epigenetic regulatory mechanisms that may enhance the efficiency of SE in pineapple and other plants. |
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| ISSN: | 2073-4425 |