Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields
Currently, microbial biofilms have been the cause of a wide variety of infections in the human body, reaching 80% of all bacterial and fungal infections. The biofilms present specific properties that increase the resistance to antimicrobial treatments. Thus, the development of new approaches is urge...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2021.1960105 |
| _version_ | 1798014852428464128 |
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| author | Wanessa de Cassia Martins Antunes de Melo Raimonda Celiešiūtė-Germanienė Povilas Šimonis Arūnas Stirkė |
| author_facet | Wanessa de Cassia Martins Antunes de Melo Raimonda Celiešiūtė-Germanienė Povilas Šimonis Arūnas Stirkė |
| author_sort | Wanessa de Cassia Martins Antunes de Melo |
| collection | DOAJ |
| description | Currently, microbial biofilms have been the cause of a wide variety of infections in the human body, reaching 80% of all bacterial and fungal infections. The biofilms present specific properties that increase the resistance to antimicrobial treatments. Thus, the development of new approaches is urgent, and antimicrobial photodynamic therapy (aPDT) has been shown as a promising candidate. aPDT involves a synergic association of a photosensitizer (PS), molecular oxygen and visible light, producing highly reactive oxygen species (ROS) that cause the oxidation of several cellular components. This therapy attacks many components of the biofilm, including proteins, lipids, and nucleic acids present within the biofilm matrix; causing inhibition even in the cells that are inside the extracellular polymeric substance (EPS). Recent advances in designing new PSs to increase the production of ROS and the combination of aPDT with other therapies, especially pulsed electric fields (PEF), have contributed to enhanced biofilm inhibition. The PEF has proven to have antimicrobial effect once it is known that extensive chemical reactions occur when electric fields are applied. This type of treatment kills microorganisms not only due to membrane rupture but also due to the formation of reactive compounds including free oxygen, hydrogen, hydroxyl and hydroperoxyl radicals. So, this review aims to show the progress of aPDT and PEF against the biofilms, suggesting that the association of both methods can potentiate their effects and overcome biofilm infections. |
| first_indexed | 2024-04-11T15:23:41Z |
| format | Article |
| id | doaj.art-8f8edb9590804d789279a22313c123c4 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-11T15:23:41Z |
| publishDate | 2021-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-8f8edb9590804d789279a22313c123c42022-12-22T04:16:18ZengTaylor & Francis GroupVirulence2150-55942150-56082021-12-011212247227210.1080/21505594.2021.19601051960105Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fieldsWanessa de Cassia Martins Antunes de Melo0Raimonda Celiešiūtė-Germanienė1Povilas Šimonis2Arūnas Stirkė3Laboratory of Bioelectric, State Research Institute, Department of Functional Materials and Electronics, Center for Physical Sciences and TechnologyLaboratory of Bioelectric, State Research Institute, Department of Functional Materials and Electronics, Center for Physical Sciences and TechnologyLaboratory of Bioelectric, State Research Institute, Department of Functional Materials and Electronics, Center for Physical Sciences and TechnologyLaboratory of Bioelectric, State Research Institute, Department of Functional Materials and Electronics, Center for Physical Sciences and TechnologyCurrently, microbial biofilms have been the cause of a wide variety of infections in the human body, reaching 80% of all bacterial and fungal infections. The biofilms present specific properties that increase the resistance to antimicrobial treatments. Thus, the development of new approaches is urgent, and antimicrobial photodynamic therapy (aPDT) has been shown as a promising candidate. aPDT involves a synergic association of a photosensitizer (PS), molecular oxygen and visible light, producing highly reactive oxygen species (ROS) that cause the oxidation of several cellular components. This therapy attacks many components of the biofilm, including proteins, lipids, and nucleic acids present within the biofilm matrix; causing inhibition even in the cells that are inside the extracellular polymeric substance (EPS). Recent advances in designing new PSs to increase the production of ROS and the combination of aPDT with other therapies, especially pulsed electric fields (PEF), have contributed to enhanced biofilm inhibition. The PEF has proven to have antimicrobial effect once it is known that extensive chemical reactions occur when electric fields are applied. This type of treatment kills microorganisms not only due to membrane rupture but also due to the formation of reactive compounds including free oxygen, hydrogen, hydroxyl and hydroperoxyl radicals. So, this review aims to show the progress of aPDT and PEF against the biofilms, suggesting that the association of both methods can potentiate their effects and overcome biofilm infections.http://dx.doi.org/10.1080/21505594.2021.1960105antimicrobial resistance1biofilms2eps3ros4apdt5pef6photosensitizer7 and electroporation8 |
| spellingShingle | Wanessa de Cassia Martins Antunes de Melo Raimonda Celiešiūtė-Germanienė Povilas Šimonis Arūnas Stirkė Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields Virulence antimicrobial resistance1 biofilms2 eps3 ros4 apdt5 pef6 photosensitizer7 and electroporation8 |
| title | Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields |
| title_full | Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields |
| title_fullStr | Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields |
| title_full_unstemmed | Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields |
| title_short | Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields |
| title_sort | antimicrobial photodynamic therapy apdt for biofilm treatments possible synergy between apdt and pulsed electric fields |
| topic | antimicrobial resistance1 biofilms2 eps3 ros4 apdt5 pef6 photosensitizer7 and electroporation8 |
| url | http://dx.doi.org/10.1080/21505594.2021.1960105 |
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