Identification and characterization of a novel multi-stress responsive gene in Arabidopsis.
Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analys...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0244030 |
| _version_ | 1818574239419072512 |
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| author | Faiza Tawab Iqbal Munir Zeeshan Nasim Mohammad Sayyar Khan Saleha Tawab Adnan Nasim Aqib Iqbal Mian Afaq Ahmad Waqar Ali Raheel Munir Maria Munir Noreen Asim |
| author_facet | Faiza Tawab Iqbal Munir Zeeshan Nasim Mohammad Sayyar Khan Saleha Tawab Adnan Nasim Aqib Iqbal Mian Afaq Ahmad Waqar Ali Raheel Munir Maria Munir Noreen Asim |
| author_sort | Faiza Tawab |
| collection | DOAJ |
| description | Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA. |
| first_indexed | 2024-12-15T00:23:56Z |
| format | Article |
| id | doaj.art-8f90771629384aba831cc5b7f74cecbd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-15T00:23:56Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8f90771629384aba831cc5b7f74cecbd2022-12-21T22:42:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011512e024403010.1371/journal.pone.0244030Identification and characterization of a novel multi-stress responsive gene in Arabidopsis.Faiza TawabIqbal MunirZeeshan NasimMohammad Sayyar KhanSaleha TawabAdnan NasimAqib IqbalMian Afaq AhmadWaqar AliRaheel MunirMaria MunirNoreen AsimAbiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.https://doi.org/10.1371/journal.pone.0244030 |
| spellingShingle | Faiza Tawab Iqbal Munir Zeeshan Nasim Mohammad Sayyar Khan Saleha Tawab Adnan Nasim Aqib Iqbal Mian Afaq Ahmad Waqar Ali Raheel Munir Maria Munir Noreen Asim Identification and characterization of a novel multi-stress responsive gene in Arabidopsis. PLoS ONE |
| title | Identification and characterization of a novel multi-stress responsive gene in Arabidopsis. |
| title_full | Identification and characterization of a novel multi-stress responsive gene in Arabidopsis. |
| title_fullStr | Identification and characterization of a novel multi-stress responsive gene in Arabidopsis. |
| title_full_unstemmed | Identification and characterization of a novel multi-stress responsive gene in Arabidopsis. |
| title_short | Identification and characterization of a novel multi-stress responsive gene in Arabidopsis. |
| title_sort | identification and characterization of a novel multi stress responsive gene in arabidopsis |
| url | https://doi.org/10.1371/journal.pone.0244030 |
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