Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi
Introduction: The province of Pichincha in Ecuador is an endemic area of cutaneous leishmaniasis, where anthropophilic sand flies with natural infection by Leishmania, have been reported as vectors. However, the role in transmission of zoophilic species has not been evaluated. Objective: To evaluate...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Nacional de Salud
2017-03-01
|
| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/3536 |
| _version_ | 1818012190530076672 |
|---|---|
| author | Jazzmín Arrivillaga-Henríquez Sandra Enríquez Vanessa Romero Gustavo Echeverría Jorge Pérez-Barrera Ana Poveda Juan-Carlos Navarro Alon Warburg Washington Benítez |
| author_facet | Jazzmín Arrivillaga-Henríquez Sandra Enríquez Vanessa Romero Gustavo Echeverría Jorge Pérez-Barrera Ana Poveda Juan-Carlos Navarro Alon Warburg Washington Benítez |
| author_sort | Jazzmín Arrivillaga-Henríquez |
| collection | DOAJ |
| description | Introduction: The province of Pichincha in Ecuador is an endemic area of cutaneous leishmaniasis, where anthropophilic sand flies with natural infection by Leishmania, have been reported as vectors. However, the role in transmission of zoophilic species has not been evaluated.
Objective: To evaluate natural infection by Leishmania in two zoophilic phlebotomine sand fly species, Lutzomyia reburra and Lu. barrettoi majuscula, and one anthropophilic species, Lu. trapidoi, as well as the endophagy and synanthropism of these species in the northwest of Pichincha.
Materials and methods: Phlebotomines were collected using CDC light traps in different habitats and altitudes with presence of cutaneous leishmaniasis. Leishmania infection was detected using genomic DNA from females of the collected sand flies. We amplified the internal transcribed spacer gene of ribosomal RNA I (ITS1), the mitochondrial topoisomerase II gene (mtTOPOII), and the nuclear topoisomerase II gene (TopoII). Percentages of positivity for Leishmania, at spatio-temporal scale, proportion of endophagy and synanthropism index were calculated.
Results: Natural infection was determined for Le. amazonensis in Lu. reburra (9.5%) and Lu. b. majuscula (23.8%), while in Lu. trapidoi we detected Le. amazonensis, Le. brazilienis and Le. naiffi-lainsoni. Phlebotomines were asynanthropic and with low endophagy.
Conclusion: Natural infection with Le. amazonensis was recorded for the first time in Lu. reburra and Lu. b. majuscula, demonstrating the importance of zoophilic phlebotomines in the maintenance of the Leishmania transmission cycle in endemic foci. |
| first_indexed | 2024-04-14T06:16:59Z |
| format | Article |
| id | doaj.art-8f908c538e5b4c58830e9796cb2d9262 |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-04-14T06:16:59Z |
| publishDate | 2017-03-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-8f908c538e5b4c58830e9796cb2d92622022-12-22T02:08:09ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572017-03-01370839710.7705/biomedica.v37i0.35362076Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoiJazzmín Arrivillaga-Henríquez0Sandra Enríquez1Vanessa Romero2Gustavo Echeverría3Jorge Pérez-Barrera4Ana Poveda5Juan-Carlos Navarro6Alon Warburg7Washington Benítez8Instituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, Ecuador Programa Prometeo, Secretaría Nacional de Educación Superior, Ciencia, Tecnología e Innovación (SENESCYT), Quito, Ecuador Área de Ambiente, Turismo Histórico Cultural, Facultad de Comunicación Social, Universidad Central del Ecuador, Quito, EcuadorInstituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, EcuadorInstituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, Ecuador Facultad de Ciencias Biológicas, Carrera de Ciencias Biológicas y Ambientales, Universidad Central del Ecuador, Quito, EcuadorInstituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, EcuadorInstituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, EcuadorInstituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, Ecuador Facultad de Ciencias Químicas, Universidad Central del Ecuador, Quito, EcuadorLaboratorio de Biodiversidad y Salud Ambiental, Facultad de Ciencias Naturales y Ambientales, Universidad Internacional SEK, Quito, EcuadorPrograma Prometeo, Secretaría Nacional de Educación Superior, Ciencia, Tecnología e Innovación (SENESCYT), Quito, Ecuador Faculty of Medicine, Department of Microbiology and Molecular Genetics; Institute of Medical Research Israel-Canada; Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University of Jerusalem, Jerusalem, IsraelInstituto de Investigación en Salud Pública y Zoonosis, Universidad Central del Ecuador, Quito, Ecuador Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, EcuadorIntroduction: The province of Pichincha in Ecuador is an endemic area of cutaneous leishmaniasis, where anthropophilic sand flies with natural infection by Leishmania, have been reported as vectors. However, the role in transmission of zoophilic species has not been evaluated. Objective: To evaluate natural infection by Leishmania in two zoophilic phlebotomine sand fly species, Lutzomyia reburra and Lu. barrettoi majuscula, and one anthropophilic species, Lu. trapidoi, as well as the endophagy and synanthropism of these species in the northwest of Pichincha. Materials and methods: Phlebotomines were collected using CDC light traps in different habitats and altitudes with presence of cutaneous leishmaniasis. Leishmania infection was detected using genomic DNA from females of the collected sand flies. We amplified the internal transcribed spacer gene of ribosomal RNA I (ITS1), the mitochondrial topoisomerase II gene (mtTOPOII), and the nuclear topoisomerase II gene (TopoII). Percentages of positivity for Leishmania, at spatio-temporal scale, proportion of endophagy and synanthropism index were calculated. Results: Natural infection was determined for Le. amazonensis in Lu. reburra (9.5%) and Lu. b. majuscula (23.8%), while in Lu. trapidoi we detected Le. amazonensis, Le. brazilienis and Le. naiffi-lainsoni. Phlebotomines were asynanthropic and with low endophagy. Conclusion: Natural infection with Le. amazonensis was recorded for the first time in Lu. reburra and Lu. b. majuscula, demonstrating the importance of zoophilic phlebotomines in the maintenance of the Leishmania transmission cycle in endemic foci.https://www.revistabiomedica.org/index.php/biomedica/article/view/3536PsychodidaeLeishmanialeishmaniasis, cutaneouspolymerase chain reactionEcuador |
| spellingShingle | Jazzmín Arrivillaga-Henríquez Sandra Enríquez Vanessa Romero Gustavo Echeverría Jorge Pérez-Barrera Ana Poveda Juan-Carlos Navarro Alon Warburg Washington Benítez Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi Biomédica: revista del Instituto Nacional de Salud Psychodidae Leishmania leishmaniasis, cutaneous polymerase chain reaction Ecuador |
| title | Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi |
| title_full | Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi |
| title_fullStr | Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi |
| title_full_unstemmed | Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi |
| title_short | Eco-epidemiological aspects, natural detection and molecular identification of Leishmania spp. in Lutzomyia reburra, Lutzomyia barrettoi majuscula and Lutzomyia trapidoi |
| title_sort | eco epidemiological aspects natural detection and molecular identification of leishmania spp in lutzomyia reburra lutzomyia barrettoi majuscula and lutzomyia trapidoi |
| topic | Psychodidae Leishmania leishmaniasis, cutaneous polymerase chain reaction Ecuador |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/3536 |
| work_keys_str_mv | AT jazzminarrivillagahenriquez ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT sandraenriquez ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT vanessaromero ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT gustavoecheverria ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT jorgeperezbarrera ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT anapoveda ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT juancarlosnavarro ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT alonwarburg ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi AT washingtonbenitez ecoepidemiologicalaspectsnaturaldetectionandmolecularidentificationofleishmaniasppinlutzomyiareburralutzomyiabarrettoimajusculaandlutzomyiatrapidoi |