Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading
<p>Cell attachment to the extracellular matrix (ECM) engages integrin signaling into the cell, but part of the signaling response also stem from cell spreading (<abbr bid="B3">3</abbr>). To analyze specific integrin signaling-mediated responses independent of cell spreadi...
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| Format: | Article |
| Language: | English |
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BMC
2002-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/37/m37.htm |
| _version_ | 1828429280712851456 |
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| author | Bao Wenjie Strömblad Staffan |
| author_facet | Bao Wenjie Strömblad Staffan |
| author_sort | Bao Wenjie |
| collection | DOAJ |
| description | <p>Cell attachment to the extracellular matrix (ECM) engages integrin signaling into the cell, but part of the signaling response also stem from cell spreading (<abbr bid="B3">3</abbr>). To analyze specific integrin signaling-mediated responses independent of cell spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin &agr;5&bgr;1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti-integrin &agr;5&bgr;1) or onto poly-L-lysin (P-L-L), which mediates integrin-independent attachment. Cells attached and spread on FN, while cells on JBS5 or P-L-L attached but did not spread. Importantly, plating onto FN or mab JBS5 gave rise to identical integrin-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21<sup>CIP1</sup> and p27<sup>KIP1</sup>, while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin &agr;5&bgr;1 induces integrin signaling regulating the Cdk2-inhibitors independent of cell spreading and present a method for how integrin signaling can be analyzed separate from the effects of cell spreading. |
| first_indexed | 2024-12-10T17:30:57Z |
| format | Article |
| id | doaj.art-8f9886c76bdd4f14bffa1303d990e8ce |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-10T17:30:57Z |
| publishDate | 2002-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-8f9886c76bdd4f14bffa1303d990e8ce2022-12-22T01:39:41ZengBMCBiological Procedures Online1480-92222002-01-0141818710.1251/bpo37Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell SpreadingBao WenjieStrömblad Staffan<p>Cell attachment to the extracellular matrix (ECM) engages integrin signaling into the cell, but part of the signaling response also stem from cell spreading (<abbr bid="B3">3</abbr>). To analyze specific integrin signaling-mediated responses independent of cell spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin &agr;5&bgr;1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti-integrin &agr;5&bgr;1) or onto poly-L-lysin (P-L-L), which mediates integrin-independent attachment. Cells attached and spread on FN, while cells on JBS5 or P-L-L attached but did not spread. Importantly, plating onto FN or mab JBS5 gave rise to identical integrin-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21<sup>CIP1</sup> and p27<sup>KIP1</sup>, while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin &agr;5&bgr;1 induces integrin signaling regulating the Cdk2-inhibitors independent of cell spreading and present a method for how integrin signaling can be analyzed separate from the effects of cell spreading.http://www.biologicalprocedures.com/bpo/arts/1/37/m37.htmcell adhesionintegrinsantibodies, monoclonalfibronectinsprotein kinases |
| spellingShingle | Bao Wenjie Strömblad Staffan Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading Biological Procedures Online cell adhesion integrins antibodies, monoclonal fibronectins protein kinases |
| title | Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading |
| title_full | Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading |
| title_fullStr | Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading |
| title_full_unstemmed | Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading |
| title_short | Use of an Immobilized Monoclonal Antibody to Examine Integrin &agr;5&bgr;1 Signaling Independent of Cell Spreading |
| title_sort | use of an immobilized monoclonal antibody to examine integrin agr 5 bgr 1 signaling independent of cell spreading |
| topic | cell adhesion integrins antibodies, monoclonal fibronectins protein kinases |
| url | http://www.biologicalprocedures.com/bpo/arts/1/37/m37.htm |
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