Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells
Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that increases glucose-stimulated insulin secretion in pancreatic β-cells. Since mitochondrial function is crucial to insulin secretion, we hypothesized that GLP-1 may increase mitochondrial biogenesis in pancreatic β-cells. We treate...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Korean Endocrine Society
2015-06-01
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| Series: | Endocrinology and Metabolism |
| Subjects: | |
| Online Access: | http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-30-216.pdf |
| _version_ | 1811322049308655616 |
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| author | Mi Yeon Kang Tae Jung Oh Young Min Cho |
| author_facet | Mi Yeon Kang Tae Jung Oh Young Min Cho |
| author_sort | Mi Yeon Kang |
| collection | DOAJ |
| description | Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that increases glucose-stimulated insulin secretion in pancreatic β-cells. Since mitochondrial function is crucial to insulin secretion, we hypothesized that GLP-1 may increase mitochondrial biogenesis in pancreatic β-cells. We treated INS-1 rat insulinoma cells with GLP-1 or exendin-4 for 48 hours and measured mitochondrial mass and function. Both GLP-1 and exendin-4 increased mitochondrial mass by approximately 20%. The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4. In addition, GLP-1 increased the mitochondrial membrane potential and oxygen consumption. Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment. In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance. |
| first_indexed | 2024-04-13T13:28:22Z |
| format | Article |
| id | doaj.art-8f9904f5ec84474e97746e219e0b7644 |
| institution | Directory Open Access Journal |
| issn | 2093-596X 2093-5978 |
| language | English |
| last_indexed | 2024-04-13T13:28:22Z |
| publishDate | 2015-06-01 |
| publisher | Korean Endocrine Society |
| record_format | Article |
| series | Endocrinology and Metabolism |
| spelling | doaj.art-8f9904f5ec84474e97746e219e0b76442022-12-22T02:45:03ZengKorean Endocrine SocietyEndocrinology and Metabolism2093-596X2093-59782015-06-0130221622010.3803/EnM.2015.30.2.21620804Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma CellsMi Yeon KangTae Jung OhYoung Min ChoGlucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone that increases glucose-stimulated insulin secretion in pancreatic β-cells. Since mitochondrial function is crucial to insulin secretion, we hypothesized that GLP-1 may increase mitochondrial biogenesis in pancreatic β-cells. We treated INS-1 rat insulinoma cells with GLP-1 or exendin-4 for 48 hours and measured mitochondrial mass and function. Both GLP-1 and exendin-4 increased mitochondrial mass by approximately 20%. The mitochondria/cytosol ratio was increased from 7.60±3.12% to 10.53±2.70% by exendin-4. In addition, GLP-1 increased the mitochondrial membrane potential and oxygen consumption. Proliferator-activated receptor-gamma coactivator 1α expression was increased approximately 2-fold by GLP-1 treatment. In conclusion, the present study presents evidence for a new mechanism of action by which GLP-1 improves pancreatic β-cell function via enhanced mitochondrial mass and performance.http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-30-216.pdfGlucagon-like peptide-1MitochondriaDiabetes mellitusBeta cellInsulin |
| spellingShingle | Mi Yeon Kang Tae Jung Oh Young Min Cho Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells Endocrinology and Metabolism Glucagon-like peptide-1 Mitochondria Diabetes mellitus Beta cell Insulin |
| title | Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells |
| title_full | Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells |
| title_fullStr | Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells |
| title_full_unstemmed | Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells |
| title_short | Glucagon-Like Peptide-1 Increases Mitochondrial Biogenesis and Function in INS-1 Rat Insulinoma Cells |
| title_sort | glucagon like peptide 1 increases mitochondrial biogenesis and function in ins 1 rat insulinoma cells |
| topic | Glucagon-like peptide-1 Mitochondria Diabetes mellitus Beta cell Insulin |
| url | http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-30-216.pdf |
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