Role of metallothionein-III following central nervous system damage
We evaluated the physiological relevance of metallothionein-III (MT-III) in the central nervous system following damage caused by a focal cryolesion onto the cortex by studying Mt3-null mice. In normal mice, dramatic astrogliosis and microgliosis and T-cell infiltration were observed in the area sur...
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| Format: | Article |
| Language: | English |
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Elsevier
2003-06-01
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| Series: | Neurobiology of Disease |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996103000159 |
| _version_ | 1818573115236548608 |
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| author | Javier Carrasco Milena Penkowa Mercedes Giralt Jordi Camats Amalia Molinero Iain L. Campbell Richard D. Palmiter Juan Hidalgo |
| author_facet | Javier Carrasco Milena Penkowa Mercedes Giralt Jordi Camats Amalia Molinero Iain L. Campbell Richard D. Palmiter Juan Hidalgo |
| author_sort | Javier Carrasco |
| collection | DOAJ |
| description | We evaluated the physiological relevance of metallothionein-III (MT-III) in the central nervous system following damage caused by a focal cryolesion onto the cortex by studying Mt3-null mice. In normal mice, dramatic astrogliosis and microgliosis and T-cell infiltration were observed in the area surrounding the lesioned tissue, along with signs of increased oxidative stress and apoptosis. There was also significant upregulation of cytokines/growth factors such as tumor necrosis factor-α, interleukin (IL)-1 α/β, and IL-6 as measured by ribonuclease protection assay. Mt3-null mice did not differ from control mice in these responses, in sharp contrast to results obtained in Mt1- Mt2-null mice. In contrast, Mt3-null mice showed increased expression of several neurotrophins as well as of the neuronal sprouting factor GAP-43. Thus, unlike MT-I and MT-II, MT-III does not affect the inflammatory response elicited in the central nervous system by a cryoinjury, nor does it serve an important antioxidant role, but it may influence neuronal regeneration during the recovery process. |
| first_indexed | 2024-12-15T00:06:46Z |
| format | Article |
| id | doaj.art-8f9a25f27563409a83d67ccb1338e788 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-15T00:06:46Z |
| publishDate | 2003-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-8f9a25f27563409a83d67ccb1338e7882022-12-21T22:42:42ZengElsevierNeurobiology of Disease1095-953X2003-06-011312236Role of metallothionein-III following central nervous system damageJavier Carrasco0Milena Penkowa1Mercedes Giralt2Jordi Camats3Amalia Molinero4Iain L. Campbell5Richard D. Palmiter6Juan Hidalgo7Institute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAInstitute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Barcelona, Spain 08193; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark; Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USAWe evaluated the physiological relevance of metallothionein-III (MT-III) in the central nervous system following damage caused by a focal cryolesion onto the cortex by studying Mt3-null mice. In normal mice, dramatic astrogliosis and microgliosis and T-cell infiltration were observed in the area surrounding the lesioned tissue, along with signs of increased oxidative stress and apoptosis. There was also significant upregulation of cytokines/growth factors such as tumor necrosis factor-α, interleukin (IL)-1 α/β, and IL-6 as measured by ribonuclease protection assay. Mt3-null mice did not differ from control mice in these responses, in sharp contrast to results obtained in Mt1- Mt2-null mice. In contrast, Mt3-null mice showed increased expression of several neurotrophins as well as of the neuronal sprouting factor GAP-43. Thus, unlike MT-I and MT-II, MT-III does not affect the inflammatory response elicited in the central nervous system by a cryoinjury, nor does it serve an important antioxidant role, but it may influence neuronal regeneration during the recovery process.http://www.sciencedirect.com/science/article/pii/S0969996103000159 |
| spellingShingle | Javier Carrasco Milena Penkowa Mercedes Giralt Jordi Camats Amalia Molinero Iain L. Campbell Richard D. Palmiter Juan Hidalgo Role of metallothionein-III following central nervous system damage Neurobiology of Disease |
| title | Role of metallothionein-III following central nervous system damage |
| title_full | Role of metallothionein-III following central nervous system damage |
| title_fullStr | Role of metallothionein-III following central nervous system damage |
| title_full_unstemmed | Role of metallothionein-III following central nervous system damage |
| title_short | Role of metallothionein-III following central nervous system damage |
| title_sort | role of metallothionein iii following central nervous system damage |
| url | http://www.sciencedirect.com/science/article/pii/S0969996103000159 |
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