Structural Insights into Iron Ions Accumulation in Dps Nanocage
Dps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. Moreover, this ferritin-like protein can perform fast oxidation of ferrous ions and subsequently accumulate c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5313 |
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| author | Yury Chesnokov Andrey Mozhaev Roman Kamyshinsky Alexander Gordienko Liubov Dadinova |
| author_facet | Yury Chesnokov Andrey Mozhaev Roman Kamyshinsky Alexander Gordienko Liubov Dadinova |
| author_sort | Yury Chesnokov |
| collection | DOAJ |
| description | Dps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. Moreover, this ferritin-like protein can perform fast oxidation of ferrous ions and subsequently accumulate clusters of ferric ions in its nanocages, thus providing the bacterium with physical and chemical protection. Here, cryo-electron microscopy was used to study the accumulation of iron ions in the nanocage of a Dps protein from <i>Escherichia coli</i>. We demonstrate that Fe<sup>2+</sup> concentration in the solution and incubation time have an insignificant effect on the volume and the morphology of iron minerals formed in Dps nanocages. However, an increase in the Fe<sup>2+</sup> level leads to an increase in the proportion of larger clusters and the clusters themselves are composed of discrete ~1–1.5 nm subunits. |
| first_indexed | 2024-03-10T03:45:31Z |
| format | Article |
| id | doaj.art-8f9adeae81cb46c29d821633c05dd9b2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:45:31Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8f9adeae81cb46c29d821633c05dd9b22023-11-23T11:20:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310531310.3390/ijms23105313Structural Insights into Iron Ions Accumulation in Dps NanocageYury Chesnokov0Andrey Mozhaev1Roman Kamyshinsky2Alexander Gordienko3Liubov Dadinova4Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, Leninskiy Prospect, 59, 119333 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, Leninskiy Prospect, 59, 119333 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, Leninskiy Prospect, 59, 119333 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, Leninskiy Prospect, 59, 119333 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” of Russian Academy of Sciences, Leninskiy Prospect, 59, 119333 Moscow, RussiaDps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. Moreover, this ferritin-like protein can perform fast oxidation of ferrous ions and subsequently accumulate clusters of ferric ions in its nanocages, thus providing the bacterium with physical and chemical protection. Here, cryo-electron microscopy was used to study the accumulation of iron ions in the nanocage of a Dps protein from <i>Escherichia coli</i>. We demonstrate that Fe<sup>2+</sup> concentration in the solution and incubation time have an insignificant effect on the volume and the morphology of iron minerals formed in Dps nanocages. However, an increase in the Fe<sup>2+</sup> level leads to an increase in the proportion of larger clusters and the clusters themselves are composed of discrete ~1–1.5 nm subunits.https://www.mdpi.com/1422-0067/23/10/5313DNA-binding protein from starved cells (Dps)iron nanoparticlesFe mineralsmini-ferritincryo-electron microscopysingle particle analysis |
| spellingShingle | Yury Chesnokov Andrey Mozhaev Roman Kamyshinsky Alexander Gordienko Liubov Dadinova Structural Insights into Iron Ions Accumulation in Dps Nanocage International Journal of Molecular Sciences DNA-binding protein from starved cells (Dps) iron nanoparticles Fe minerals mini-ferritin cryo-electron microscopy single particle analysis |
| title | Structural Insights into Iron Ions Accumulation in Dps Nanocage |
| title_full | Structural Insights into Iron Ions Accumulation in Dps Nanocage |
| title_fullStr | Structural Insights into Iron Ions Accumulation in Dps Nanocage |
| title_full_unstemmed | Structural Insights into Iron Ions Accumulation in Dps Nanocage |
| title_short | Structural Insights into Iron Ions Accumulation in Dps Nanocage |
| title_sort | structural insights into iron ions accumulation in dps nanocage |
| topic | DNA-binding protein from starved cells (Dps) iron nanoparticles Fe minerals mini-ferritin cryo-electron microscopy single particle analysis |
| url | https://www.mdpi.com/1422-0067/23/10/5313 |
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