Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress
Rough lemon (Citrus jambhiri Lush.) – RL, is used as a rootstock for citrus plants in saline conditions. NaCl causes an osmotic stress on plants mainly preventing the water uptake by the roots and thus reducing the plant growth. The objective of this study was to determine the effects of salicylic...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2023-04-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4798 |
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| author | Murat Güneri Zeynel Dalkılıç |
| author_facet | Murat Güneri Zeynel Dalkılıç |
| author_sort | Murat Güneri |
| collection | DOAJ |
| description |
Rough lemon (Citrus jambhiri Lush.) – RL, is used as a rootstock for citrus plants in saline conditions. NaCl causes an osmotic stress on plants mainly preventing the water uptake by the roots and thus reducing the plant growth. The objective of this study was to determine the effects of salicylic acid (SA) on germination of seeds and the growth and development of seedlings of RL rootstock under salt stress. For seed germination, a study was conducted in a completely randomized design in a 4 × 4 factorial scheme (SA at 0.00, 0.25, 0.50, or 1.00 mM and NaCl at 0, 50, 100, or 200 mM) with 4 repetitions, totaling 64 plots, of 25 seeds per plot. RL seeds were incubated in SA solutions for 24 h. Then, they were treated with NaCl-containing water in Petri dishes and incubated in the growth chamber at 25°C. For greenhouse experiment, a study was conducted in a randomized complete block in a 4 × 4 factorial scheme (SA at 0.0, 0.5, 1.0, or 2.0 mM and NaCl at 0, 50, 100, or 150 mM) with 3 repetitions, totaling 48 plots, of 2 plants per plot. Some morphological and physiological characteristics were determined. While germination time was extended, germination ratio and radicle extension were decreased in seeds under salt stress compared to control. Moreover, in these conditions the leaf membrane permeability and leaf falling were increased. In turn, plant height, diameter, root and shoot dry weight, leaf relative water content, and leaf chlorophyll were decreased in seedlings. Salt stress had negatively affected seed germination from 97.5% in control to 23.5% in 200 mM NaCl. However, SA treatments significantly decreased plant height to 67.8 cm in 2.0 mM compared to 80.1 cm in control in RL rootstock.
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| first_indexed | 2024-03-09T00:14:22Z |
| format | Article |
| id | doaj.art-8f9c87906fdf4e2db4a328c3526631ea |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:14:22Z |
| publishDate | 2023-04-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-8f9c87906fdf4e2db4a328c3526631ea2023-12-12T09:39:49ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052023-04-0122210.24326/asphc.2023.4798Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stressMurat Güneri0Zeynel Dalkılıç1Muğla Sıtkı Koçman University, Ortaca Vocational School, Horticulture Program, Muğla 48600, Turkey Aydın Adnan Menderes University, Faculty of Agriculture, Department of Horticulture, 09100 Aydın, Turkey Rough lemon (Citrus jambhiri Lush.) – RL, is used as a rootstock for citrus plants in saline conditions. NaCl causes an osmotic stress on plants mainly preventing the water uptake by the roots and thus reducing the plant growth. The objective of this study was to determine the effects of salicylic acid (SA) on germination of seeds and the growth and development of seedlings of RL rootstock under salt stress. For seed germination, a study was conducted in a completely randomized design in a 4 × 4 factorial scheme (SA at 0.00, 0.25, 0.50, or 1.00 mM and NaCl at 0, 50, 100, or 200 mM) with 4 repetitions, totaling 64 plots, of 25 seeds per plot. RL seeds were incubated in SA solutions for 24 h. Then, they were treated with NaCl-containing water in Petri dishes and incubated in the growth chamber at 25°C. For greenhouse experiment, a study was conducted in a randomized complete block in a 4 × 4 factorial scheme (SA at 0.0, 0.5, 1.0, or 2.0 mM and NaCl at 0, 50, 100, or 150 mM) with 3 repetitions, totaling 48 plots, of 2 plants per plot. Some morphological and physiological characteristics were determined. While germination time was extended, germination ratio and radicle extension were decreased in seeds under salt stress compared to control. Moreover, in these conditions the leaf membrane permeability and leaf falling were increased. In turn, plant height, diameter, root and shoot dry weight, leaf relative water content, and leaf chlorophyll were decreased in seedlings. Salt stress had negatively affected seed germination from 97.5% in control to 23.5% in 200 mM NaCl. However, SA treatments significantly decreased plant height to 67.8 cm in 2.0 mM compared to 80.1 cm in control in RL rootstock. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4798Citrus jambhirisalinityseed germinationplant growth and developmentstress tolerance |
| spellingShingle | Murat Güneri Zeynel Dalkılıç Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress Acta Scientiarum Polonorum: Hortorum Cultus Citrus jambhiri salinity seed germination plant growth and development stress tolerance |
| title | Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress |
| title_full | Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress |
| title_fullStr | Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress |
| title_full_unstemmed | Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress |
| title_short | Effects of salicylic acid application on germination, growth and development of rough lemon (Citrus jambhiri Lush.) under salt stress |
| title_sort | effects of salicylic acid application on germination growth and development of rough lemon citrus jambhiri lush under salt stress |
| topic | Citrus jambhiri salinity seed germination plant growth and development stress tolerance |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4798 |
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