Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods.
We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle ce...
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| Format: | Article |
| Language: | English |
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Via Medica
2004-10-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4646 |
| _version_ | 1811318689013694464 |
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| author | Zbigniew Puchalski Jacek Dadan Małgorzata Barwijuk-Machała Janusz Dziecioł Albert Augustynowicz Stanisław Sulkowski |
| author_facet | Zbigniew Puchalski Jacek Dadan Małgorzata Barwijuk-Machała Janusz Dziecioł Albert Augustynowicz Stanisław Sulkowski |
| author_sort | Zbigniew Puchalski |
| collection | DOAJ |
| description | We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle cell tumors: 20 HĂźrthle cell carcinomas (HCC), 32 HĂźrthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67. AgNOR dot count in the nucleus and proliferative index (PI - percentage of cells expressing PCNA and Ki-67) in randomly chosen nuclei (100 in case of AgNOR and over 1000 in case of PI) were evaluated in each slide. The mean values of AgNOR dot count, PI-PCNA and PI-Ki-67 in HCC, HCA and HCM were respectively: 5.1, 61.3 and 54.9; 3.4, 42.4 and 38.6 and 2.5, 39.3 and 34.3. Statistically significant difference was found in all the proliferative activity markers between malignant and benign tumors: HCC:HCA (p<0.01) and HCC:HCM (p<0.001). There was no statistically significant difference between HCA and HCM. |
| first_indexed | 2024-04-13T12:29:02Z |
| format | Article |
| id | doaj.art-8f9e50bc89d241bea1c8461fbd5f911c |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-04-13T12:29:02Z |
| publishDate | 2004-10-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-8f9e50bc89d241bea1c8461fbd5f911c2022-12-22T02:46:54ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312004-10-0142316516810.5603/4646Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods.Zbigniew PuchalskiJacek DadanMałgorzata Barwijuk-MachałaJanusz DzieciołAlbert AugustynowiczStanisław SulkowskiWe have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid HĂźrthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 HĂźrthle cell tumors: 20 HĂźrthle cell carcinomas (HCC), 32 HĂźrthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67. AgNOR dot count in the nucleus and proliferative index (PI - percentage of cells expressing PCNA and Ki-67) in randomly chosen nuclei (100 in case of AgNOR and over 1000 in case of PI) were evaluated in each slide. The mean values of AgNOR dot count, PI-PCNA and PI-Ki-67 in HCC, HCA and HCM were respectively: 5.1, 61.3 and 54.9; 3.4, 42.4 and 38.6 and 2.5, 39.3 and 34.3. Statistically significant difference was found in all the proliferative activity markers between malignant and benign tumors: HCC:HCA (p<0.01) and HCC:HCM (p<0.001). There was no statistically significant difference between HCA and HCM.http://czasopisma.viamedica.pl/fhc/article/view/4646 |
| spellingShingle | Zbigniew Puchalski Jacek Dadan Małgorzata Barwijuk-Machała Janusz Dziecioł Albert Augustynowicz Stanisław Sulkowski Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods. Folia Histochemica et Cytobiologica |
| title | Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods. |
| title_full | Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods. |
| title_fullStr | Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods. |
| title_full_unstemmed | Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods. |
| title_short | Assessment of proliferative activity of thyroid HĂźrthle cell tumors using PCNA, Ki-67 and AgNOR methods. |
| title_sort | assessment of proliferative activity of thyroid hazrthle cell tumors using pcna ki 67 and agnor methods |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4646 |
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