Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel
The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short com...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-10-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2022.2027359 |
| _version_ | 1827816767937839104 |
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| author | Marie-Astrid van Dievoet Davide Brusa Marie Octave Sandrine Horman Xavier Stephenne |
| author_facet | Marie-Astrid van Dievoet Davide Brusa Marie Octave Sandrine Horman Xavier Stephenne |
| author_sort | Marie-Astrid van Dievoet |
| collection | DOAJ |
| description | The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies. |
| first_indexed | 2024-03-12T00:25:20Z |
| format | Article |
| id | doaj.art-8f9fe7c2e11d40f0b1f7f3e1b58ec5af |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:25:20Z |
| publishDate | 2022-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-8f9fe7c2e11d40f0b1f7f3e1b58ec5af2023-09-15T10:38:10ZengTaylor & Francis GroupPlatelets0953-71041369-16352022-10-013371096109910.1080/09537104.2022.20273592027359Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panelMarie-Astrid van Dievoet0Davide Brusa1Marie Octave2Sandrine Horman3Xavier Stephenne4Université Catholique de Louvain (Uclouvain)UCLouvainUCLouvainUCLouvainUniversité Catholique de Louvain (Uclouvain)The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies.http://dx.doi.org/10.1080/09537104.2022.2027359anti-gpviflow cytometryhy101platelet |
| spellingShingle | Marie-Astrid van Dievoet Davide Brusa Marie Octave Sandrine Horman Xavier Stephenne Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel Platelets anti-gpvi flow cytometry hy101 platelet |
| title | Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel |
| title_full | Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel |
| title_fullStr | Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel |
| title_full_unstemmed | Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel |
| title_short | Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel |
| title_sort | anti gpvi hy101 activates platelets in a multicolor flow cytometry panel |
| topic | anti-gpvi flow cytometry hy101 platelet |
| url | http://dx.doi.org/10.1080/09537104.2022.2027359 |
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