Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes
Endophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects to the host plants. In a previous study, we isolated eight bacterial endophytes from sweet potato plants harvested in Salyan, Nepal. These endophytes showed plant growth-promoting properties as a mixed c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/4/4/53 |
| _version_ | 1819195816082931712 |
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| author | Sabitri Adhikari Dhungana Fumihiko Adachi Shohei Hayashi Ramesh Raj Puri Kazuhito Itoh |
| author_facet | Sabitri Adhikari Dhungana Fumihiko Adachi Shohei Hayashi Ramesh Raj Puri Kazuhito Itoh |
| author_sort | Sabitri Adhikari Dhungana |
| collection | DOAJ |
| description | Endophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects to the host plants. In a previous study, we isolated eight bacterial endophytes from sweet potato plants harvested in Salyan, Nepal. These endophytes showed plant growth-promoting properties as a mixed culture. In this study, we evaluated the ability of these strains to produce indole-3-acetic acid (IAA) and to fix nitrogen. Based on these results, we selected two strains, <i>Klebsiella</i> sp. Sal 1 and <i>Enterobacter</i> sp. Sal 3, and evaluated their ability to promote plant growth. IAA production activity peaked at 15⁻60 mg NH<sub>4</sub>NO<sub>3</sub>/L in plant-free medium. Similarly, acetylene reduction activity peaked at 0⁻6.25 mg NH<sub>4</sub>NO<sub>3</sub>/L. Both strains successfully colonized plants, promoted the growth of tomatoes, and induced phenotypes in plants consistent with IAA exposure. This suggests that these strains promote plant growth by producing IAA inside the plant, where nitrogen levels are expected to be low. |
| first_indexed | 2024-12-23T02:18:46Z |
| format | Article |
| id | doaj.art-8fa54fbf4964490992324a8185acb070 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-12-23T02:18:46Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-8fa54fbf4964490992324a8185acb0702022-12-21T18:03:35ZengMDPI AGHorticulturae2311-75242018-12-01445310.3390/horticulturae4040053horticulturae4040053Plant Growth Promoting Effects of Nepalese Sweet Potato EndophytesSabitri Adhikari Dhungana0Fumihiko Adachi1Shohei Hayashi2Ramesh Raj Puri3Kazuhito Itoh4The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, JapanFaculty of Life and Environmental Sciences, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, JapanFaculty of Life and Environmental Sciences, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, JapanThe United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, JapanThe United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, JapanEndophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects to the host plants. In a previous study, we isolated eight bacterial endophytes from sweet potato plants harvested in Salyan, Nepal. These endophytes showed plant growth-promoting properties as a mixed culture. In this study, we evaluated the ability of these strains to produce indole-3-acetic acid (IAA) and to fix nitrogen. Based on these results, we selected two strains, <i>Klebsiella</i> sp. Sal 1 and <i>Enterobacter</i> sp. Sal 3, and evaluated their ability to promote plant growth. IAA production activity peaked at 15⁻60 mg NH<sub>4</sub>NO<sub>3</sub>/L in plant-free medium. Similarly, acetylene reduction activity peaked at 0⁻6.25 mg NH<sub>4</sub>NO<sub>3</sub>/L. Both strains successfully colonized plants, promoted the growth of tomatoes, and induced phenotypes in plants consistent with IAA exposure. This suggests that these strains promote plant growth by producing IAA inside the plant, where nitrogen levels are expected to be low.https://www.mdpi.com/2311-7524/4/4/53endophyteindole-3-acetic acid (IAA)sweet potatotomatonitrogen fixationcolonization |
| spellingShingle | Sabitri Adhikari Dhungana Fumihiko Adachi Shohei Hayashi Ramesh Raj Puri Kazuhito Itoh Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes Horticulturae endophyte indole-3-acetic acid (IAA) sweet potato tomato nitrogen fixation colonization |
| title | Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes |
| title_full | Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes |
| title_fullStr | Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes |
| title_full_unstemmed | Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes |
| title_short | Plant Growth Promoting Effects of Nepalese Sweet Potato Endophytes |
| title_sort | plant growth promoting effects of nepalese sweet potato endophytes |
| topic | endophyte indole-3-acetic acid (IAA) sweet potato tomato nitrogen fixation colonization |
| url | https://www.mdpi.com/2311-7524/4/4/53 |
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