Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia
Acute myeloid leukemia (AML) is the most lethal leukemia with an extremely poor prognosis and high relapse rates. In leukemogenesis, adhesion abnormalities can readily guide an imbalance between hematopoietic progenitor cells and bone marrow stromal cells, altering the normal hematopoietic bone marr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | Biomaterials and Biosystems |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666534422000071 |
| _version_ | 1828349434715439104 |
|---|---|
| author | Sameh A. Mohammed Yasuhiro Kimura Yuhki Toku Yang Ju |
| author_facet | Sameh A. Mohammed Yasuhiro Kimura Yuhki Toku Yang Ju |
| author_sort | Sameh A. Mohammed |
| collection | DOAJ |
| description | Acute myeloid leukemia (AML) is the most lethal leukemia with an extremely poor prognosis and high relapse rates. In leukemogenesis, adhesion abnormalities can readily guide an imbalance between hematopoietic progenitor cells and bone marrow stromal cells, altering the normal hematopoietic bone marrow microenvironment into leukemic transformation that enhances leukemic proliferation. Here, we have firstly studied the PLEKHA7 expression in leukemic cells to assess their growth capability affected by the restoration of PLEKHA7 in the cells. The efficacy of PLEKHA7-loaded cRGD-mediated PEGylated cationic lipid nanoparticles for efficient PLEKHA7 delivery in leukemic cells as well as the effect of PLEKHA7 on the regulated induction of AML behavior and growth alterations were investigated. PLEKHA7 re-expression diminished colony-forming ability and reinforced the incidence of growth retardation without apoptosis in AML cell lines. PLEKHA7 regulated the restoration of cell surface adhesion and integrity during normal homeostasis. Our findings revealed that PLEKHA7 functions as a behavior and growth modulator in AML. To our knowledge, the role of PLEKHA7 in AML had not been studied previously and our data could be exploited for further mechanistic studies and insights into altering human AML behavior and growth. |
| first_indexed | 2024-04-14T01:09:29Z |
| format | Article |
| id | doaj.art-8fa631ca22024aa8be020520c010c75c |
| institution | Directory Open Access Journal |
| issn | 2666-5344 |
| language | English |
| last_indexed | 2024-04-14T01:09:29Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomaterials and Biosystems |
| spelling | doaj.art-8fa631ca22024aa8be020520c010c75c2022-12-22T02:21:08ZengElsevierBiomaterials and Biosystems2666-53442022-06-016100045Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemiaSameh A. Mohammed0Yasuhiro Kimura1Yuhki Toku2Yang Ju3Department of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University; Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, EgyptDepartment of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University; Furo-cho, Chikusa-ku, Nagoya, 464-8603, JapanDepartment of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University; Furo-cho, Chikusa-ku, Nagoya, 464-8603, JapanDepartment of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University; Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan; Corresponding author at: Department of Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.Acute myeloid leukemia (AML) is the most lethal leukemia with an extremely poor prognosis and high relapse rates. In leukemogenesis, adhesion abnormalities can readily guide an imbalance between hematopoietic progenitor cells and bone marrow stromal cells, altering the normal hematopoietic bone marrow microenvironment into leukemic transformation that enhances leukemic proliferation. Here, we have firstly studied the PLEKHA7 expression in leukemic cells to assess their growth capability affected by the restoration of PLEKHA7 in the cells. The efficacy of PLEKHA7-loaded cRGD-mediated PEGylated cationic lipid nanoparticles for efficient PLEKHA7 delivery in leukemic cells as well as the effect of PLEKHA7 on the regulated induction of AML behavior and growth alterations were investigated. PLEKHA7 re-expression diminished colony-forming ability and reinforced the incidence of growth retardation without apoptosis in AML cell lines. PLEKHA7 regulated the restoration of cell surface adhesion and integrity during normal homeostasis. Our findings revealed that PLEKHA7 functions as a behavior and growth modulator in AML. To our knowledge, the role of PLEKHA7 in AML had not been studied previously and our data could be exploited for further mechanistic studies and insights into altering human AML behavior and growth.http://www.sciencedirect.com/science/article/pii/S2666534422000071Acute myeloid leukemiaPLEKHA7NanoparticlesModulatorNormal homeostasis |
| spellingShingle | Sameh A. Mohammed Yasuhiro Kimura Yuhki Toku Yang Ju Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia Biomaterials and Biosystems Acute myeloid leukemia PLEKHA7 Nanoparticles Modulator Normal homeostasis |
| title | Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia |
| title_full | Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia |
| title_fullStr | Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia |
| title_full_unstemmed | Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia |
| title_short | Bioengineered PLEKHA7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia |
| title_sort | bioengineered plekha7 nanodelivery regularly induces behavior alteration and growth retardation of acute myeloid leukemia |
| topic | Acute myeloid leukemia PLEKHA7 Nanoparticles Modulator Normal homeostasis |
| url | http://www.sciencedirect.com/science/article/pii/S2666534422000071 |
| work_keys_str_mv | AT samehamohammed bioengineeredplekha7nanodeliveryregularlyinducesbehavioralterationandgrowthretardationofacutemyeloidleukemia AT yasuhirokimura bioengineeredplekha7nanodeliveryregularlyinducesbehavioralterationandgrowthretardationofacutemyeloidleukemia AT yuhkitoku bioengineeredplekha7nanodeliveryregularlyinducesbehavioralterationandgrowthretardationofacutemyeloidleukemia AT yangju bioengineeredplekha7nanodeliveryregularlyinducesbehavioralterationandgrowthretardationofacutemyeloidleukemia |