Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds
A comparative study of bioactive lipids extraction from black cumin (<i>Nigella sativa</i> L.) and basil (<i>Ocimum basilicum</i> L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obt...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/7/1493 |
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| author | Soumaya Bourgou Iness Bettaieb Rebey Sofiene Ben Kaab Majdi Hammami Sarra Dakhlaoui Selmi Sawsen Kamel Msaada Hiroko Isoda Riadh Ksouri Marie-Laure Fauconnier |
| author_facet | Soumaya Bourgou Iness Bettaieb Rebey Sofiene Ben Kaab Majdi Hammami Sarra Dakhlaoui Selmi Sawsen Kamel Msaada Hiroko Isoda Riadh Ksouri Marie-Laure Fauconnier |
| author_sort | Soumaya Bourgou |
| collection | DOAJ |
| description | A comparative study of bioactive lipids extraction from black cumin (<i>Nigella sativa</i> L.) and basil (<i>Ocimum basilicum</i> L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, <i>trans</i>-hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities. |
| first_indexed | 2024-03-10T10:00:49Z |
| format | Article |
| id | doaj.art-8fa95025abd74bd4a81ba438dcb4900d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T10:00:49Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-8fa95025abd74bd4a81ba438dcb4900d2023-11-22T01:58:39ZengMDPI AGFoods2304-81582021-06-01107149310.3390/foods10071493Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil SeedsSoumaya Bourgou0Iness Bettaieb Rebey1Sofiene Ben Kaab2Majdi Hammami3Sarra Dakhlaoui4Selmi Sawsen5Kamel Msaada6Hiroko Isoda7Riadh Ksouri8Marie-Laure Fauconnier9Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaFaculty of Life and Environmental Sciences, School of Integrative and Global Majors (SIGMA), and Alliance for Research on Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba 3058572, JapanLaboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030 Gembloux, BelgiumA comparative study of bioactive lipids extraction from black cumin (<i>Nigella sativa</i> L.) and basil (<i>Ocimum basilicum</i> L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, <i>trans</i>-hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities.https://www.mdpi.com/2304-8158/10/7/1493<i>Nigella sativa</i><i>Ocimum basilicum</i>PUFAtocopherolsphenolicsgreen extraction |
| spellingShingle | Soumaya Bourgou Iness Bettaieb Rebey Sofiene Ben Kaab Majdi Hammami Sarra Dakhlaoui Selmi Sawsen Kamel Msaada Hiroko Isoda Riadh Ksouri Marie-Laure Fauconnier Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds Foods <i>Nigella sativa</i> <i>Ocimum basilicum</i> PUFA tocopherols phenolics green extraction |
| title | Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds |
| title_full | Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds |
| title_fullStr | Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds |
| title_full_unstemmed | Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds |
| title_short | Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds |
| title_sort | green solvent to substitute hexane for bioactive lipids extraction from black cumin and basil seeds |
| topic | <i>Nigella sativa</i> <i>Ocimum basilicum</i> PUFA tocopherols phenolics green extraction |
| url | https://www.mdpi.com/2304-8158/10/7/1493 |
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