Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma
Objective: Allergic asthma is a complex inflammatory disorder that affects the airways. As an ancient medical system, Iranian Traditional Medicine (ITM) recommends a polyherbal formula called “Monzej-e-balgham” for the treatment of asthma. In the present investigation, the antiasthmatic effects of “...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2022-09-01
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| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | https://ajp.mums.ac.ir/article_20050_a9b4f046d19c85e83bad3cc38ad13e1f.pdf |
| Summary: | Objective: Allergic asthma is a complex inflammatory disorder that affects the airways. As an ancient medical system, Iranian Traditional Medicine (ITM) recommends a polyherbal formula called “Monzej-e-balgham” for the treatment of asthma. In the present investigation, the antiasthmatic effects of “Monzej-e-balgham” were examined in a murine model of allergic asthma.Materials and Methods: Twenty-eight Balb/c mice weighing 15-20 g were allocated into 4 groups. As negative and positive controls, groups I and II received phosphate-buffered saline (PBS) and ovalbumin (OVA) solutions, respectively. Groups III and IV were first sensitized with OVA and then respectively treated with “Monzej-e-balgham” (63 mg/kg) and budesonide. Finally, bronchoalveolar lavage fluid (BALF) and lung tissues of the animals were collected and used for eosinophil counting, Th2 type interleukins (IL-5, IL-13, and IL-33) measurement, and histological examinations.Results: “Monzej-e-balgham” significantly reduced the number of eosinophils and the levels of IL-5, IL-13, and IL-33 in BALF specimens compared to OVA-sensitized group (p<0.05). It also ameliorated histopathological changes of the lung tissues such as goblet cells hyperplasia and mucus overproduction in comparison to group II. Interestingly, the results of the “Monzej-e-balgham”-treated group were comparable with those obtained for budesonide-inhaled mice.Conclusion: The present data indicated a mechanism that involves Th2 inflammatory responses in allergic asthma and suggested a polyherbal mixture for the treatment of this disease. |
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| ISSN: | 2228-7930 2228-7949 |