Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma
Objective: Allergic asthma is a complex inflammatory disorder that affects the airways. As an ancient medical system, Iranian Traditional Medicine (ITM) recommends a polyherbal formula called “Monzej-e-balgham” for the treatment of asthma. In the present investigation, the antiasthmatic effects of “...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2022-09-01
|
| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | https://ajp.mums.ac.ir/article_20050_a9b4f046d19c85e83bad3cc38ad13e1f.pdf |
| _version_ | 1811216029717626880 |
|---|---|
| author | Maryam Hamzeloo-Moghadam Sayyed Shamsadin Athari Hanieh Kashafroodi Tahereh Dargahi Leila Ara Rasool Choopani |
| author_facet | Maryam Hamzeloo-Moghadam Sayyed Shamsadin Athari Hanieh Kashafroodi Tahereh Dargahi Leila Ara Rasool Choopani |
| author_sort | Maryam Hamzeloo-Moghadam |
| collection | DOAJ |
| description | Objective: Allergic asthma is a complex inflammatory disorder that affects the airways. As an ancient medical system, Iranian Traditional Medicine (ITM) recommends a polyherbal formula called “Monzej-e-balgham” for the treatment of asthma. In the present investigation, the antiasthmatic effects of “Monzej-e-balgham” were examined in a murine model of allergic asthma.Materials and Methods: Twenty-eight Balb/c mice weighing 15-20 g were allocated into 4 groups. As negative and positive controls, groups I and II received phosphate-buffered saline (PBS) and ovalbumin (OVA) solutions, respectively. Groups III and IV were first sensitized with OVA and then respectively treated with “Monzej-e-balgham” (63 mg/kg) and budesonide. Finally, bronchoalveolar lavage fluid (BALF) and lung tissues of the animals were collected and used for eosinophil counting, Th2 type interleukins (IL-5, IL-13, and IL-33) measurement, and histological examinations.Results: “Monzej-e-balgham” significantly reduced the number of eosinophils and the levels of IL-5, IL-13, and IL-33 in BALF specimens compared to OVA-sensitized group (p<0.05). It also ameliorated histopathological changes of the lung tissues such as goblet cells hyperplasia and mucus overproduction in comparison to group II. Interestingly, the results of the “Monzej-e-balgham”-treated group were comparable with those obtained for budesonide-inhaled mice.Conclusion: The present data indicated a mechanism that involves Th2 inflammatory responses in allergic asthma and suggested a polyherbal mixture for the treatment of this disease. |
| first_indexed | 2024-04-12T06:32:39Z |
| format | Article |
| id | doaj.art-8faa1e4c237f44d4b6891637c371e107 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-04-12T06:32:39Z |
| publishDate | 2022-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-8faa1e4c237f44d4b6891637c371e1072022-12-22T03:43:59ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492022-09-0112552753610.22038/ajp.2022.2005020050Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthmaMaryam Hamzeloo-Moghadam0Sayyed Shamsadin Athari1Hanieh Kashafroodi2Tahereh Dargahi3Leila Ara4Rasool Choopani5Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical SciencesDepartment of Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, IranTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Objective: Allergic asthma is a complex inflammatory disorder that affects the airways. As an ancient medical system, Iranian Traditional Medicine (ITM) recommends a polyherbal formula called “Monzej-e-balgham” for the treatment of asthma. In the present investigation, the antiasthmatic effects of “Monzej-e-balgham” were examined in a murine model of allergic asthma.Materials and Methods: Twenty-eight Balb/c mice weighing 15-20 g were allocated into 4 groups. As negative and positive controls, groups I and II received phosphate-buffered saline (PBS) and ovalbumin (OVA) solutions, respectively. Groups III and IV were first sensitized with OVA and then respectively treated with “Monzej-e-balgham” (63 mg/kg) and budesonide. Finally, bronchoalveolar lavage fluid (BALF) and lung tissues of the animals were collected and used for eosinophil counting, Th2 type interleukins (IL-5, IL-13, and IL-33) measurement, and histological examinations.Results: “Monzej-e-balgham” significantly reduced the number of eosinophils and the levels of IL-5, IL-13, and IL-33 in BALF specimens compared to OVA-sensitized group (p<0.05). It also ameliorated histopathological changes of the lung tissues such as goblet cells hyperplasia and mucus overproduction in comparison to group II. Interestingly, the results of the “Monzej-e-balgham”-treated group were comparable with those obtained for budesonide-inhaled mice.Conclusion: The present data indicated a mechanism that involves Th2 inflammatory responses in allergic asthma and suggested a polyherbal mixture for the treatment of this disease.https://ajp.mums.ac.ir/article_20050_a9b4f046d19c85e83bad3cc38ad13e1f.pdfallergic asthmabalb/c micemonzej-e-balghamiranian traditional medicine |
| spellingShingle | Maryam Hamzeloo-Moghadam Sayyed Shamsadin Athari Hanieh Kashafroodi Tahereh Dargahi Leila Ara Rasool Choopani Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma Avicenna Journal of Phytomedicine allergic asthma balb/c mice monzej-e-balgham iranian traditional medicine |
| title | Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma |
| title_full | Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma |
| title_fullStr | Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma |
| title_full_unstemmed | Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma |
| title_short | Effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin-induced allergic asthma |
| title_sort | effects of a polyherbal formulation on inflammation and histopathological alterations in mice with ovalbumin induced allergic asthma |
| topic | allergic asthma balb/c mice monzej-e-balgham iranian traditional medicine |
| url | https://ajp.mums.ac.ir/article_20050_a9b4f046d19c85e83bad3cc38ad13e1f.pdf |
| work_keys_str_mv | AT maryamhamzeloomoghadam effectsofapolyherbalformulationoninflammationandhistopathologicalalterationsinmicewithovalbumininducedallergicasthma AT sayyedshamsadinathari effectsofapolyherbalformulationoninflammationandhistopathologicalalterationsinmicewithovalbumininducedallergicasthma AT haniehkashafroodi effectsofapolyherbalformulationoninflammationandhistopathologicalalterationsinmicewithovalbumininducedallergicasthma AT taherehdargahi effectsofapolyherbalformulationoninflammationandhistopathologicalalterationsinmicewithovalbumininducedallergicasthma AT leilaara effectsofapolyherbalformulationoninflammationandhistopathologicalalterationsinmicewithovalbumininducedallergicasthma AT rasoolchoopani effectsofapolyherbalformulationoninflammationandhistopathologicalalterationsinmicewithovalbumininducedallergicasthma |