Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces
<p/> <p>In the autumn of 1995 the first outbreaks of enterohemorrhagic <it>Escherichia coli </it>O157:H7 including ca 100 human cases were reported in Sweden. From outbreaks in other countries it is known that cattle may carry these bacteria and in many cases is the source of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-03-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://www.actavetscand.com/content/44/1/43 |
| _version_ | 1818262964669513728 |
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| author | Aspán A Wallen C Eriksson E Albihn A |
| author_facet | Aspán A Wallen C Eriksson E Albihn A |
| author_sort | Aspán A |
| collection | DOAJ |
| description | <p/> <p>In the autumn of 1995 the first outbreaks of enterohemorrhagic <it>Escherichia coli </it>O157:H7 including ca 100 human cases were reported in Sweden. From outbreaks in other countries it is known that cattle may carry these bacteria and in many cases is the source of infection. Therefore, the present study was performed to survey the Swedish bovine population for the presence of verotoxin-producing <it>E. coli </it>(VTEC) of serotype O157:H7. Individual faecal samples were collected at the 16 main Swedish abattoirs from April 1996 to August 1997. Of 3071 faecal samples, VTEC O157 were found in 37 samples indicating a prevalence of 1.2% (CI<sub>95% </sub>0.8–1.6). All 37 isolates carried genes encoding for verotoxin (VT1 and/or VT2), intimin, EHEC-haemolysin and flagellin H7 as determined by PCR. Another 3 strains were of serotype O157:H7 but did not produce verotoxins. The 37 VTEC O157:H7 strains were further characterised by phage typing and pulsed-field gel electrophoresis. The results clearly show that VTEC O157:H7 is established in the Swedish bovine population and indicate that the prevalence of cattle carrying VTEC O157:H7 is correlated to the overall geographical distribution of cattle in Sweden. Results of this study have formed the basis for specific measures recommended to Swedish cattle farmers, and furthermore, a permanent monitoring programme was launched for VTEC O157:H7 in Swedish cattle at slaughter.</p> |
| first_indexed | 2024-12-12T19:11:29Z |
| format | Article |
| id | doaj.art-8fab41a2317642eab8f3e3d665737c7b |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-12T19:11:29Z |
| publishDate | 2003-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-8fab41a2317642eab8f3e3d665737c7b2022-12-22T00:14:51ZengBMCActa Veterinaria Scandinavica1751-01472003-03-01441435210.1186/1751-0147-44-43Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine FaecesAspán AWallen CEriksson EAlbihn A<p/> <p>In the autumn of 1995 the first outbreaks of enterohemorrhagic <it>Escherichia coli </it>O157:H7 including ca 100 human cases were reported in Sweden. From outbreaks in other countries it is known that cattle may carry these bacteria and in many cases is the source of infection. Therefore, the present study was performed to survey the Swedish bovine population for the presence of verotoxin-producing <it>E. coli </it>(VTEC) of serotype O157:H7. Individual faecal samples were collected at the 16 main Swedish abattoirs from April 1996 to August 1997. Of 3071 faecal samples, VTEC O157 were found in 37 samples indicating a prevalence of 1.2% (CI<sub>95% </sub>0.8–1.6). All 37 isolates carried genes encoding for verotoxin (VT1 and/or VT2), intimin, EHEC-haemolysin and flagellin H7 as determined by PCR. Another 3 strains were of serotype O157:H7 but did not produce verotoxins. The 37 VTEC O157:H7 strains were further characterised by phage typing and pulsed-field gel electrophoresis. The results clearly show that VTEC O157:H7 is established in the Swedish bovine population and indicate that the prevalence of cattle carrying VTEC O157:H7 is correlated to the overall geographical distribution of cattle in Sweden. Results of this study have formed the basis for specific measures recommended to Swedish cattle farmers, and furthermore, a permanent monitoring programme was launched for VTEC O157:H7 in Swedish cattle at slaughter.</p>http://www.actavetscand.com/content/44/1/43between phage typingPFGE |
| spellingShingle | Aspán A Wallen C Eriksson E Albihn A Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces Acta Veterinaria Scandinavica between phage typing PFGE |
| title | Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces |
| title_full | Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces |
| title_fullStr | Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces |
| title_full_unstemmed | Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces |
| title_short | Verotoxinogenic <it>Escherichia coli </it>(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces |
| title_sort | verotoxinogenic it escherichia coli it vtec o157 h7 a nationwide swedish survey of bovine faeces |
| topic | between phage typing PFGE |
| url | http://www.actavetscand.com/content/44/1/43 |
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