Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups
The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1999-05-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000300008 |
| _version_ | 1797756714470080512 |
|---|---|
| author | Brigitte Bastrenta Marie France Bosseno Christian Barnabé Michel Tibayrenc Simone Frédérique Brenière |
| author_facet | Brigitte Bastrenta Marie France Bosseno Christian Barnabé Michel Tibayrenc Simone Frédérique Brenière |
| author_sort | Brigitte Bastrenta |
| collection | DOAJ |
| description | The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high molecular weight DNA (Hae III), while group 2 presents a ladder profile for each enzyme, which is a characteristic of tandemly repeated DNA. The two groups, respectively, clustered stocks pertaining to the two principal lineages evidenced by isoenzyme and RAPD markers. The congruence among these three independent genomic markers corroborates the existence of two real phylogenetic lineages in T. cruzi. The specific monomorphic profiles for each major phylogenetic lineage suggest the existence of ancient sexuality and cryptic biological speciation. |
| first_indexed | 2024-03-12T18:05:29Z |
| format | Article |
| id | doaj.art-8fabc5be4eb84eca8c619fcbf66bf7bb |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:05:29Z |
| publishDate | 1999-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-8fabc5be4eb84eca8c619fcbf66bf7bb2023-08-02T09:28:34ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601999-05-0194332332810.1590/S0074-02761999000300008Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groupsBrigitte BastrentaMarie France BossenoChristian BarnabéMichel TibayrencSimone Frédérique BrenièreThe restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high molecular weight DNA (Hae III), while group 2 presents a ladder profile for each enzyme, which is a characteristic of tandemly repeated DNA. The two groups, respectively, clustered stocks pertaining to the two principal lineages evidenced by isoenzyme and RAPD markers. The congruence among these three independent genomic markers corroborates the existence of two real phylogenetic lineages in T. cruzi. The specific monomorphic profiles for each major phylogenetic lineage suggest the existence of ancient sexuality and cryptic biological speciation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000300008Trypanosoma cruzisatellite DNA195 bp repeat unit |
| spellingShingle | Brigitte Bastrenta Marie France Bosseno Christian Barnabé Michel Tibayrenc Simone Frédérique Brenière Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi satellite DNA 195 bp repeat unit |
| title | Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups |
| title_full | Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups |
| title_fullStr | Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups |
| title_full_unstemmed | Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups |
| title_short | Restriction fragment length polymorphism of 195 bp repeated satellite dna of Trypanosoma cruzi supports the existence of two phylogenetic groups |
| title_sort | restriction fragment length polymorphism of 195 bp repeated satellite dna of trypanosoma cruzi supports the existence of two phylogenetic groups |
| topic | Trypanosoma cruzi satellite DNA 195 bp repeat unit |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000300008 |
| work_keys_str_mv | AT brigittebastrenta restrictionfragmentlengthpolymorphismof195bprepeatedsatellitednaoftrypanosomacruzisupportstheexistenceoftwophylogeneticgroups AT mariefrancebosseno restrictionfragmentlengthpolymorphismof195bprepeatedsatellitednaoftrypanosomacruzisupportstheexistenceoftwophylogeneticgroups AT christianbarnabe restrictionfragmentlengthpolymorphismof195bprepeatedsatellitednaoftrypanosomacruzisupportstheexistenceoftwophylogeneticgroups AT micheltibayrenc restrictionfragmentlengthpolymorphismof195bprepeatedsatellitednaoftrypanosomacruzisupportstheexistenceoftwophylogeneticgroups AT simonefrederiquebreniere restrictionfragmentlengthpolymorphismof195bprepeatedsatellitednaoftrypanosomacruzisupportstheexistenceoftwophylogeneticgroups |