Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon
Exogenous ethylene is commonly utilized to initiate flower induction in pineapple (<i>Ananas comosus</i> (L.) Merr.). However, the molecular mechanisms and metabolic changes involved are not well understood. In this study, we explored the genetic network and metabolic shifts in the ‘Comt...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/24/17133 |
| _version_ | 1797380778007461888 |
|---|---|
| author | Wenqiu Lin Shenghui Liu Xiou Xiao Weisheng Sun Xinhua Lu Yuyao Gao Junjun He Zhuying Zhu Qingsong Wu Xiumei Zhang |
| author_facet | Wenqiu Lin Shenghui Liu Xiou Xiao Weisheng Sun Xinhua Lu Yuyao Gao Junjun He Zhuying Zhu Qingsong Wu Xiumei Zhang |
| author_sort | Wenqiu Lin |
| collection | DOAJ |
| description | Exogenous ethylene is commonly utilized to initiate flower induction in pineapple (<i>Ananas comosus</i> (L.) Merr.). However, the molecular mechanisms and metabolic changes involved are not well understood. In this study, we explored the genetic network and metabolic shifts in the ‘Comte de Paris’ pineapple variety during ethylene-induced flowering. This was achieved through an integrative analysis of metabolome and transcriptome profiles at vegetative shoot apexes (0 d after ethephon treatment named BL_0d), the stage of bract primordia (8 d after ethephon treatment named BL_8d), stage of flower primordia (18 d after ethephon treatment named BL_18d), and the stage of stopped floret differentiation (34 d after ethephon treatment named BL_34d). We isolated and identified 804 metabolites in the pineapple shoot apex and inflorescence, categorized into 24 classes. Notably, 29, 31, and 46 metabolites showed significant changes from BL_0d to BL_8d, BL_8d to BL_18d, and BL_18d to BL_34d, respectively. A marked decrease in indole was observed, suggesting its role as a characteristic metabolite during flower induction. Transcriptomic analysis revealed 956, 1768, and 4483 differentially expressed genes (DEGs) for BL_0d vs. BL_8d, BL_8d vs. BL_18d, and BL_18d vs. BL_34d, respectively. These DEGs were significantly enriched in carbohydrate metabolism and hormone signaling pathways, indicating their potential involvement in flower induction. Integrating metabolomic and transcriptomic data, we identified several candidate genes, such as <i>Agamous-Like9</i> (<i>AGL9</i>), <i>Ethylene Insensitive 3-like</i> (<i>ETIL3</i>), <i>Apetala2</i> (<i>AP2</i>), <i>AP2-like ethylene-responsive transcription factor ANT</i> (<i>ANT</i>), and <i>Sucrose synthase 2</i> (<i>SS2</i>), that play potentially crucial roles in ethylene-induced flower induction in pineapple. We also established a regulatory network for pineapple flower induction, correlating metabolites and DEGs, based on the <i>Arabidopsis thaliana</i> pathway as a reference. Overall, our findings offer a deeper understanding of the metabolomic and molecular mechanisms driving pineapple flowering. |
| first_indexed | 2024-03-08T20:42:54Z |
| format | Article |
| id | doaj.art-8fabe723a5c14224bb5b9ee1a773c45c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:42:54Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8fabe723a5c14224bb5b9ee1a773c45c2023-12-22T14:13:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241713310.3390/ijms242417133Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by EthephonWenqiu Lin0Shenghui Liu1Xiou Xiao2Weisheng Sun3Xinhua Lu4Yuyao Gao5Junjun He6Zhuying Zhu7Qingsong Wu8Xiumei Zhang9South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaExogenous ethylene is commonly utilized to initiate flower induction in pineapple (<i>Ananas comosus</i> (L.) Merr.). However, the molecular mechanisms and metabolic changes involved are not well understood. In this study, we explored the genetic network and metabolic shifts in the ‘Comte de Paris’ pineapple variety during ethylene-induced flowering. This was achieved through an integrative analysis of metabolome and transcriptome profiles at vegetative shoot apexes (0 d after ethephon treatment named BL_0d), the stage of bract primordia (8 d after ethephon treatment named BL_8d), stage of flower primordia (18 d after ethephon treatment named BL_18d), and the stage of stopped floret differentiation (34 d after ethephon treatment named BL_34d). We isolated and identified 804 metabolites in the pineapple shoot apex and inflorescence, categorized into 24 classes. Notably, 29, 31, and 46 metabolites showed significant changes from BL_0d to BL_8d, BL_8d to BL_18d, and BL_18d to BL_34d, respectively. A marked decrease in indole was observed, suggesting its role as a characteristic metabolite during flower induction. Transcriptomic analysis revealed 956, 1768, and 4483 differentially expressed genes (DEGs) for BL_0d vs. BL_8d, BL_8d vs. BL_18d, and BL_18d vs. BL_34d, respectively. These DEGs were significantly enriched in carbohydrate metabolism and hormone signaling pathways, indicating their potential involvement in flower induction. Integrating metabolomic and transcriptomic data, we identified several candidate genes, such as <i>Agamous-Like9</i> (<i>AGL9</i>), <i>Ethylene Insensitive 3-like</i> (<i>ETIL3</i>), <i>Apetala2</i> (<i>AP2</i>), <i>AP2-like ethylene-responsive transcription factor ANT</i> (<i>ANT</i>), and <i>Sucrose synthase 2</i> (<i>SS2</i>), that play potentially crucial roles in ethylene-induced flower induction in pineapple. We also established a regulatory network for pineapple flower induction, correlating metabolites and DEGs, based on the <i>Arabidopsis thaliana</i> pathway as a reference. Overall, our findings offer a deeper understanding of the metabolomic and molecular mechanisms driving pineapple flowering.https://www.mdpi.com/1422-0067/24/24/17133pineappleflower inductionmetabolometranscriptomeregulatory network |
| spellingShingle | Wenqiu Lin Shenghui Liu Xiou Xiao Weisheng Sun Xinhua Lu Yuyao Gao Junjun He Zhuying Zhu Qingsong Wu Xiumei Zhang Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon International Journal of Molecular Sciences pineapple flower induction metabolome transcriptome regulatory network |
| title | Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon |
| title_full | Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon |
| title_fullStr | Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon |
| title_full_unstemmed | Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon |
| title_short | Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (<i>Ananas comosus</i> (L.) Merr.) by Ethephon |
| title_sort | integrative analysis of metabolome and transcriptome provides insights into the mechanism of flower induction in pineapple i ananas comosus i l merr by ethephon |
| topic | pineapple flower induction metabolome transcriptome regulatory network |
| url | https://www.mdpi.com/1422-0067/24/24/17133 |
| work_keys_str_mv | AT wenqiulin integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT shenghuiliu integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT xiouxiao integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT weishengsun integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT xinhualu integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT yuyaogao integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT junjunhe integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT zhuyingzhu integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT qingsongwu integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon AT xiumeizhang integrativeanalysisofmetabolomeandtranscriptomeprovidesinsightsintothemechanismofflowerinductioninpineappleiananascomosusilmerrbyethephon |