Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern
Colostrum protein, an essential source of dietary nutrients, could improve new-born animals" immunity, and play a vital role in mammals" early development. In order to explore the milk yield and colostrum whey protein composition of Tibetan sheep, 120 Tibetan sheep were arbitrarily separat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-10-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3050 |
| _version_ | 1797403851938070528 |
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| author | Hao REN Qian CHEN Yingying ZHANG Huaixia ZHANG Jianlei JIA |
| author_facet | Hao REN Qian CHEN Yingying ZHANG Huaixia ZHANG Jianlei JIA |
| author_sort | Hao REN |
| collection | DOAJ |
| description | Colostrum protein, an essential source of dietary nutrients, could improve new-born
animals" immunity, and play a vital role in mammals" early development. In order to
explore the milk yield and colostrum whey protein composition of Tibetan sheep, 120
Tibetan sheep were arbitrarily separated into categories, namely treatment groups
(A, B, C) and control group (D). Compositional and functional differences in milk
yield and colostrum whey protein composition among different dietary proteins were
compared using proteomics methods. The results showed that sheep with 14% protein
level diet group (group B) had the least bodyweight loss and higher milk yield during
lactation compared to the other groups. Fifty differentially expressed proteins (DEPs)
were recognized using iTRAQ, these DEPs were analyzed based on cluster, GO, KEGG
and PPIs analysis. GO-BP involved were Protein transmembrane transport, Protein
regulation metabolic process, Biological regulation, Regulation of biological process,
and Response to stimulus. Meantime, DEPs participated in many KEGG pathways,
including Fatty acid metabolism, Glycerophospholipid metabolism, Protein digestion
and absorption, Ras signaling pathway and Cell adhesion molecules. The treatment
groups showed increase in the abundance of regulation metabolic process (especially
protein metabolism and fatty acid metabolism), along with decrease in stress reaction
process. Lactoferrin, Alpha-S2-casein, Superoxide dismutase [Cu-Zn], Alpha-s1-casein,
Alpha globin and Lactoperoxidase appeared in the center of the PPi network intersection.
Interestingly, 14% protein group (group B) had exhibited the greatest variability between
biological relevance in milk composition and function, these results could increase the
understanding of different dietary protein on colostrum whey protein composition of
Tibetan sheep, which could provide important information and potential directions for
the infant milk powder and functional food industries. |
| first_indexed | 2024-03-09T02:44:27Z |
| format | Article |
| id | doaj.art-8fb0d620f0ec49d6b3141007781be853 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-09T02:44:27Z |
| publishDate | 2023-10-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-8fb0d620f0ec49d6b3141007781be8532023-12-05T21:27:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-10-0129664965710.9775/kvfd.2023.301593050Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed patternHao RENQian CHEN0Yingying ZHANG1Huaixia ZHANG2Jianlei JIA3School of Life Science, Qilu Normal University, Jinan, Shandong, 250200, PR CHINACollege of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, PR CHINACollege of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, PR CHINASchool of Life Science, Qilu Normal University, Jinan, Shandong, 250200, PR CHINAColostrum protein, an essential source of dietary nutrients, could improve new-born animals" immunity, and play a vital role in mammals" early development. In order to explore the milk yield and colostrum whey protein composition of Tibetan sheep, 120 Tibetan sheep were arbitrarily separated into categories, namely treatment groups (A, B, C) and control group (D). Compositional and functional differences in milk yield and colostrum whey protein composition among different dietary proteins were compared using proteomics methods. The results showed that sheep with 14% protein level diet group (group B) had the least bodyweight loss and higher milk yield during lactation compared to the other groups. Fifty differentially expressed proteins (DEPs) were recognized using iTRAQ, these DEPs were analyzed based on cluster, GO, KEGG and PPIs analysis. GO-BP involved were Protein transmembrane transport, Protein regulation metabolic process, Biological regulation, Regulation of biological process, and Response to stimulus. Meantime, DEPs participated in many KEGG pathways, including Fatty acid metabolism, Glycerophospholipid metabolism, Protein digestion and absorption, Ras signaling pathway and Cell adhesion molecules. The treatment groups showed increase in the abundance of regulation metabolic process (especially protein metabolism and fatty acid metabolism), along with decrease in stress reaction process. Lactoferrin, Alpha-S2-casein, Superoxide dismutase [Cu-Zn], Alpha-s1-casein, Alpha globin and Lactoperoxidase appeared in the center of the PPi network intersection. Interestingly, 14% protein group (group B) had exhibited the greatest variability between biological relevance in milk composition and function, these results could increase the understanding of different dietary protein on colostrum whey protein composition of Tibetan sheep, which could provide important information and potential directions for the infant milk powder and functional food industries.https://vetdergikafkas.org/pdf.php?id=3050tibetan sheepdietary proteinmilk yieldcolostrum whey proteinproteomics |
| spellingShingle | Hao REN Qian CHEN Yingying ZHANG Huaixia ZHANG Jianlei JIA Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern Kafkas Universitesi Veteriner Fakültesi Dergisi tibetan sheep dietary protein milk yield colostrum whey protein proteomics |
| title | Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern |
| title_full | Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern |
| title_fullStr | Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern |
| title_full_unstemmed | Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern |
| title_short | Effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive-fed pattern |
| title_sort | effects of dietary protein on milk yield and colostrum whey protein composition of tibetan sheep in modern intensive fed pattern |
| topic | tibetan sheep dietary protein milk yield colostrum whey protein proteomics |
| url | https://vetdergikafkas.org/pdf.php?id=3050 |
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