Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients
Background Dendrobium officinale Kimura et Migo, a member of the genus Dendrobium, is a traditional Chinese medicine with high commercial value. The positive roles of UV-B radiation on active ingredient metabolism in various medicinal plants have been studied. However, the metabolic responses of D....
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PeerJ Inc.
2020-06-01
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| Online Access: | https://peerj.com/articles/9107.pdf |
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| author | Yue Chen Qi Shen Ping Lv Chongbo Sun |
| author_facet | Yue Chen Qi Shen Ping Lv Chongbo Sun |
| author_sort | Yue Chen |
| collection | DOAJ |
| description | Background Dendrobium officinale Kimura et Migo, a member of the genus Dendrobium, is a traditional Chinese medicine with high commercial value. The positive roles of UV-B radiation on active ingredient metabolism in various medicinal plants have been studied. However, the metabolic responses of D. officinale stems to UV-B treatment is largely unknown. Methods An untargeted metabolomics method was used to investigate the metabolic variations in D. officinale stems between the control and UV-B treatments. Results In total, 3,655 annotated metabolites, including 640 up- and 783 down-regulated metabolites, were identified and grouped into various primary metabolic categories. Then, a number of metabolites involved in the polysaccharide, alkaloid and flavonoid biosynthesis pathways were identified. For polysaccharide biosynthesis, several intermediate products, such as pyruvate, secologanate, tryptophan and secologanin, were significantly up-regulated by the UV-B treatment. For polysaccharide biosynthesis, many key fundamental building blocks, from the glycolysis, starch and sucrose metabolism, and fructose and mannose metabolism pathways, were induced by the UV-B treatment. For flavonoid metabolism, accumulations of several intermediate products of chalcone synthase, chalcone isomerase and flavanone 3-hydroxylase were affected by the UV-B treatment, indicating an involvement of UV-B in flavonoid biosynthesis. The UV-B induced accumulation of polysaccharides, alkaloids and flavonoids was confirmed by HPLC analysis. Our study will help to understand the effects of UV-B on the accumulation of active ingredients in D. officinale. |
| first_indexed | 2024-03-09T06:57:18Z |
| format | Article |
| id | doaj.art-8fb126a8dfc8418dba9736fc62fc16eb |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:57:18Z |
| publishDate | 2020-06-01 |
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| spelling | doaj.art-8fb126a8dfc8418dba9736fc62fc16eb2023-12-03T10:02:38ZengPeerJ Inc.PeerJ2167-83592020-06-018e910710.7717/peerj.9107Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredientsYue Chen0Qi Shen1Ping Lv2Chongbo Sun3Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, ChinaPlant Protection and Microbiology, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, ChinaAgro Technical Extension and Service Center, Hangzhou, ChinaInstitute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, ChinaBackground Dendrobium officinale Kimura et Migo, a member of the genus Dendrobium, is a traditional Chinese medicine with high commercial value. The positive roles of UV-B radiation on active ingredient metabolism in various medicinal plants have been studied. However, the metabolic responses of D. officinale stems to UV-B treatment is largely unknown. Methods An untargeted metabolomics method was used to investigate the metabolic variations in D. officinale stems between the control and UV-B treatments. Results In total, 3,655 annotated metabolites, including 640 up- and 783 down-regulated metabolites, were identified and grouped into various primary metabolic categories. Then, a number of metabolites involved in the polysaccharide, alkaloid and flavonoid biosynthesis pathways were identified. For polysaccharide biosynthesis, several intermediate products, such as pyruvate, secologanate, tryptophan and secologanin, were significantly up-regulated by the UV-B treatment. For polysaccharide biosynthesis, many key fundamental building blocks, from the glycolysis, starch and sucrose metabolism, and fructose and mannose metabolism pathways, were induced by the UV-B treatment. For flavonoid metabolism, accumulations of several intermediate products of chalcone synthase, chalcone isomerase and flavanone 3-hydroxylase were affected by the UV-B treatment, indicating an involvement of UV-B in flavonoid biosynthesis. The UV-B induced accumulation of polysaccharides, alkaloids and flavonoids was confirmed by HPLC analysis. Our study will help to understand the effects of UV-B on the accumulation of active ingredients in D. officinale.https://peerj.com/articles/9107.pdfActive ingredientDendrobiumMetabolomeMetaboliteUV-B |
| spellingShingle | Yue Chen Qi Shen Ping Lv Chongbo Sun Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients PeerJ Active ingredient Dendrobium Metabolome Metabolite UV-B |
| title | Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients |
| title_full | Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients |
| title_fullStr | Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients |
| title_full_unstemmed | Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients |
| title_short | Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients |
| title_sort | comparative metabolomic analyses of dendrobium officinale kimura et migo responding to uv b radiation reveal variations in the metabolisms associated with its bioactive ingredients |
| topic | Active ingredient Dendrobium Metabolome Metabolite UV-B |
| url | https://peerj.com/articles/9107.pdf |
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