Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties
Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxan...
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MDPI AG
2020-12-01
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| author | Michaela Nováková Tereza Fábryová Doris Vokurková Iva Dolečková Jiří Kopecký Pavel Hrouzek Lenka Tůmová José Cheel |
| author_facet | Michaela Nováková Tereza Fábryová Doris Vokurková Iva Dolečková Jiří Kopecký Pavel Hrouzek Lenka Tůmová José Cheel |
| author_sort | Michaela Nováková |
| collection | DOAJ |
| description | Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium <i>Synechocystis salina</i> (<i>S. salina</i>) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of <i>n</i>-heptane–ethanol–water (2:4:4, <i>v</i>/<i>v</i>/<i>v</i>) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health. |
| first_indexed | 2024-03-10T14:04:03Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-10T14:04:03Z |
| publishDate | 2020-12-01 |
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| series | Separations |
| spelling | doaj.art-8fb98ccc042e40ddbe05bf3c0f3b41de2023-11-21T00:50:30ZengMDPI AGSeparations2297-87392020-12-01747310.3390/separations7040073Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating PropertiesMichaela Nováková0Tereza Fábryová1Doris Vokurková2Iva Dolečková3Jiří Kopecký4Pavel Hrouzek5Lenka Tůmová6José Cheel7Laboratory of Algal Biotechnology—Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech RepublicLaboratory of Algal Biotechnology—Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech RepublicInstitute of Clinical Immunology and Allergology, Faculty of Medicine and University Hospital, Charles University, Sokolská 581, 500 05 Hradec Králové, Czech RepublicContipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czech RepublicLaboratory of Algal Biotechnology—Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech RepublicLaboratory of Algal Biotechnology—Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech RepublicDepartment of Pharmacognosy, Faculty of Pharmacy, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech RepublicLaboratory of Algal Biotechnology—Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech RepublicGlobal demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium <i>Synechocystis salina</i> (<i>S. salina</i>) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of <i>n</i>-heptane–ethanol–water (2:4:4, <i>v</i>/<i>v</i>/<i>v</i>) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health.https://www.mdpi.com/2297-8739/7/4/73myxoxanthophyllcyanobacteria<i>Synechocystis</i>high-performance countercurrent chromatography (HPCCC)countercurrent chromatography (CCC)centrifugal partition chromatography (CPC) |
| spellingShingle | Michaela Nováková Tereza Fábryová Doris Vokurková Iva Dolečková Jiří Kopecký Pavel Hrouzek Lenka Tůmová José Cheel Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties Separations myxoxanthophyll cyanobacteria <i>Synechocystis</i> high-performance countercurrent chromatography (HPCCC) countercurrent chromatography (CCC) centrifugal partition chromatography (CPC) |
| title | Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties |
| title_full | Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties |
| title_fullStr | Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties |
| title_full_unstemmed | Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties |
| title_short | Separation of the Glycosylated Carotenoid Myxoxanthophyll from <i>Synechocystis Salina</i> by HPCCC and Evaluation of Its Antioxidant, Tyrosinase Inhibitory and Immune-Stimulating Properties |
| title_sort | separation of the glycosylated carotenoid myxoxanthophyll from i synechocystis salina i by hpccc and evaluation of its antioxidant tyrosinase inhibitory and immune stimulating properties |
| topic | myxoxanthophyll cyanobacteria <i>Synechocystis</i> high-performance countercurrent chromatography (HPCCC) countercurrent chromatography (CCC) centrifugal partition chromatography (CPC) |
| url | https://www.mdpi.com/2297-8739/7/4/73 |
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