The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats
The present study assessed the changes in faecal microbial activity in obese Wistar rats fed high-fat or low-fat diets supplemented with various forms of chromium (picolinate or nanoparticles). The 18-week study was divided into two phases: an introductory period (9 weeks; obesity status induction v...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2072-6643/15/18/3962 |
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| author | Jerzy Juśkiewicz Katarzyna Ognik Joanna Fotschki Dorota Napiórkowska Ewelina Cholewińska Katarzyna Grzelak-Błaszczyk Magdalena Krauze Bartosz Fotschki |
| author_facet | Jerzy Juśkiewicz Katarzyna Ognik Joanna Fotschki Dorota Napiórkowska Ewelina Cholewińska Katarzyna Grzelak-Błaszczyk Magdalena Krauze Bartosz Fotschki |
| author_sort | Jerzy Juśkiewicz |
| collection | DOAJ |
| description | The present study assessed the changes in faecal microbial activity in obese Wistar rats fed high-fat or low-fat diets supplemented with various forms of chromium (picolinate or nanoparticles). The 18-week study was divided into two phases: an introductory period (9 weeks; obesity status induction via a high-fat diet) and an experimental period (9 weeks; maintained on a high-fat diet or switched to a low-fat diet and Cr supplementation). During the experimental period (10–18 weeks of feeding), samples of fresh faeces were collected on chosen days. The bacterial enzymatic activity and short-chain fatty acids (SCFAs) concentration were assessed to characterise the dynamism of the changes in faecal microbial metabolic activity under the applied dietary treatments. The results indicated that faecal microbial metabolic activity displayed several adaptation mechanisms in response to modifications in dietary conditions, and a beneficial outcome resulted from a pro-healthy dietary habit change, that is, switching from a high-fat to a low-fat diet. Dietary supplementation with chromium nanoparticles further modulated the aforementioned microbial activity, i.e., diminished the extracellular and total enzymatic activities, while the effect of chromium picolinate addition was negligible. Both the high-fat diet and the addition of chromium nanoparticles reduced SCFA concentrations and increased the faecal pH values. |
| first_indexed | 2024-03-10T22:20:29Z |
| format | Article |
| id | doaj.art-8fba328008774259b848436dd848b053 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T22:20:29Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-8fba328008774259b848436dd848b0532023-11-19T12:18:21ZengMDPI AGNutrients2072-66432023-09-011518396210.3390/nu15183962The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of RatsJerzy Juśkiewicz0Katarzyna Ognik1Joanna Fotschki2Dorota Napiórkowska3Ewelina Cholewińska4Katarzyna Grzelak-Błaszczyk5Magdalena Krauze6Bartosz Fotschki7Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, PolandDivision of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, PolandDivision of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, PolandInstitute of Food Technology and Analysis, Łódź University of Technology, Stefanowskiego 2/22, 90-537 Łódź, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, PolandDivision of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, PolandThe present study assessed the changes in faecal microbial activity in obese Wistar rats fed high-fat or low-fat diets supplemented with various forms of chromium (picolinate or nanoparticles). The 18-week study was divided into two phases: an introductory period (9 weeks; obesity status induction via a high-fat diet) and an experimental period (9 weeks; maintained on a high-fat diet or switched to a low-fat diet and Cr supplementation). During the experimental period (10–18 weeks of feeding), samples of fresh faeces were collected on chosen days. The bacterial enzymatic activity and short-chain fatty acids (SCFAs) concentration were assessed to characterise the dynamism of the changes in faecal microbial metabolic activity under the applied dietary treatments. The results indicated that faecal microbial metabolic activity displayed several adaptation mechanisms in response to modifications in dietary conditions, and a beneficial outcome resulted from a pro-healthy dietary habit change, that is, switching from a high-fat to a low-fat diet. Dietary supplementation with chromium nanoparticles further modulated the aforementioned microbial activity, i.e., diminished the extracellular and total enzymatic activities, while the effect of chromium picolinate addition was negligible. Both the high-fat diet and the addition of chromium nanoparticles reduced SCFA concentrations and increased the faecal pH values.https://www.mdpi.com/2072-6643/15/18/3962high-fat dietdietary habitschromium supplementationnanoparticlesfaecal microbial enzymesSCFAs |
| spellingShingle | Jerzy Juśkiewicz Katarzyna Ognik Joanna Fotschki Dorota Napiórkowska Ewelina Cholewińska Katarzyna Grzelak-Błaszczyk Magdalena Krauze Bartosz Fotschki The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats Nutrients high-fat diet dietary habits chromium supplementation nanoparticles faecal microbial enzymes SCFAs |
| title | The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats |
| title_full | The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats |
| title_fullStr | The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats |
| title_full_unstemmed | The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats |
| title_short | The Effects of Dietary Chromium Supplementation along with Discontinuing a High-Fat Diet on the Microbial Enzymatic Activity and the Production of SCFAs in the Faeces of Rats |
| title_sort | effects of dietary chromium supplementation along with discontinuing a high fat diet on the microbial enzymatic activity and the production of scfas in the faeces of rats |
| topic | high-fat diet dietary habits chromium supplementation nanoparticles faecal microbial enzymes SCFAs |
| url | https://www.mdpi.com/2072-6643/15/18/3962 |
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