The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i>
<i>Camellia oleifera</i> is the native economic edible oil tree in China and anthracnose occurs commonly in its producing areas. We previously found that ubiquitin-like CfAtg8-related autophagy is essential for the pathogenicity of <i>Colletotrichum fructicola</i>, the major...
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MDPI AG
2023-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/5/1237 |
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| author | Yan Chen Juan Jin Yiting Li Han Jiao Lan Luo Qingtian Chen He Li Shengpei Zhang |
| author_facet | Yan Chen Juan Jin Yiting Li Han Jiao Lan Luo Qingtian Chen He Li Shengpei Zhang |
| author_sort | Yan Chen |
| collection | DOAJ |
| description | <i>Camellia oleifera</i> is the native economic edible oil tree in China and anthracnose occurs commonly in its producing areas. We previously found that ubiquitin-like CfAtg8-related autophagy is essential for the pathogenicity of <i>Colletotrichum fructicola</i>, the major pathogen of anthracnose on <i>C. oleifera</i>. The aim of this study is to further elucidate the roles of autophagy in the pathogenesis of <i>C. fructicola</i>. Based on BLAST_P analysis and reverse genetics, we identified the central hub of another ubiquitin-like system, CfAtg5, and characterized its functions in <i>C. fructicola</i>. As suspected, we found that CfAtg5 is required for autophagy, both in MM-N and H<sub>2</sub>O<sub>2</sub> induction conditions. Targeted gene deletion of <i>CfATG5</i> revealed that CfAtg5 is involved in growth and conidiation. We further found that the ∆<i>Cfatg5</i> mutant is defective in appressorium formation and in responses to cell wall integrity and oxidative stresses. The combined effects, together with the abolished autophagy, result in the pathogenicity defects of the ∆<i>Cfatg5</i> mutant. Our study further illustrates the importance of normal autophagy in the physiology and pathogenicity of <i>C. fructicola</i>, and offers a potential target in the development of new anthracnose control strategies. |
| first_indexed | 2024-03-11T04:01:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T04:01:43Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8fba546a30784641a193e8c4cd5859702023-11-18T00:05:09ZengMDPI AGAgronomy2073-43952023-04-01135123710.3390/agronomy13051237The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i>Yan Chen0Juan Jin1Yiting Li2Han Jiao3Lan Luo4Qingtian Chen5He Li6Shengpei Zhang7College of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Forestry, Central South University of Forestry and Technology, Changsha 410004, China<i>Camellia oleifera</i> is the native economic edible oil tree in China and anthracnose occurs commonly in its producing areas. We previously found that ubiquitin-like CfAtg8-related autophagy is essential for the pathogenicity of <i>Colletotrichum fructicola</i>, the major pathogen of anthracnose on <i>C. oleifera</i>. The aim of this study is to further elucidate the roles of autophagy in the pathogenesis of <i>C. fructicola</i>. Based on BLAST_P analysis and reverse genetics, we identified the central hub of another ubiquitin-like system, CfAtg5, and characterized its functions in <i>C. fructicola</i>. As suspected, we found that CfAtg5 is required for autophagy, both in MM-N and H<sub>2</sub>O<sub>2</sub> induction conditions. Targeted gene deletion of <i>CfATG5</i> revealed that CfAtg5 is involved in growth and conidiation. We further found that the ∆<i>Cfatg5</i> mutant is defective in appressorium formation and in responses to cell wall integrity and oxidative stresses. The combined effects, together with the abolished autophagy, result in the pathogenicity defects of the ∆<i>Cfatg5</i> mutant. Our study further illustrates the importance of normal autophagy in the physiology and pathogenicity of <i>C. fructicola</i>, and offers a potential target in the development of new anthracnose control strategies.https://www.mdpi.com/2073-4395/13/5/1237autophagyCfAtg5pathogenicity<i>C. fructicola</i> |
| spellingShingle | Yan Chen Juan Jin Yiting Li Han Jiao Lan Luo Qingtian Chen He Li Shengpei Zhang The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i> Agronomy autophagy CfAtg5 pathogenicity <i>C. fructicola</i> |
| title | The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i> |
| title_full | The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i> |
| title_fullStr | The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i> |
| title_full_unstemmed | The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i> |
| title_short | The CfAtg5 Regulates the Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i> on <i>Camellia oleifera</i> |
| title_sort | cfatg5 regulates the autophagy and pathogenicity of i colletotrichum fructicola i on i camellia oleifera i |
| topic | autophagy CfAtg5 pathogenicity <i>C. fructicola</i> |
| url | https://www.mdpi.com/2073-4395/13/5/1237 |
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