TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
NAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNA...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.956921/full |
| _version_ | 1817997877175123968 |
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| author | Uzma Majeed Jian Hou Chenyang Hao Xueyong Zhang |
| author_facet | Uzma Majeed Jian Hou Chenyang Hao Xueyong Zhang |
| author_sort | Uzma Majeed |
| collection | DOAJ |
| description | NAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNAC020s were predominantly expressed in developing grains. The developed transgenic rice lines for TaNAC020-B showed higher starch density and lower amylose contents than those of the wild type (WT). Sequence polymorphism studies showed seven and eight SNPs in TaNAC020-A/B, making three and two haplotypes, respectively. No sequence polymorphism was identified in TaNAC020-D. Association analysis revealed that HAP-2 of TaNAC020-A and TaNAC020-B was the favored haplotype for higher thousand kernel weight and length. Geographic distribution and allelic frequency showed that our favored haplotype experienced strong selection in China, and likewise, diversity increased in TaNAC020s during wheat polyploidization. The results obtained in this study demonstrate that TaNAC020s positively influence starch synthesis and accumulation and are one of the key regulators of the kernel (seed) size and kernel number and have the potential for utilization in wheat breeding to improve grain yield. Molecular markers developed in this study stand instrumental in marker-assisted selection for genetic improvement and germplasm enhancement in wheat. |
| first_indexed | 2024-04-14T02:44:49Z |
| format | Article |
| id | doaj.art-8fbc0cab98a948118a88d1f083ec18c1 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-14T02:44:49Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-8fbc0cab98a948118a88d1f083ec18c12022-12-22T02:16:37ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-08-011310.3389/fgene.2022.956921956921TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheatUzma MajeedJian HouChenyang HaoXueyong ZhangNAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNAC020s were predominantly expressed in developing grains. The developed transgenic rice lines for TaNAC020-B showed higher starch density and lower amylose contents than those of the wild type (WT). Sequence polymorphism studies showed seven and eight SNPs in TaNAC020-A/B, making three and two haplotypes, respectively. No sequence polymorphism was identified in TaNAC020-D. Association analysis revealed that HAP-2 of TaNAC020-A and TaNAC020-B was the favored haplotype for higher thousand kernel weight and length. Geographic distribution and allelic frequency showed that our favored haplotype experienced strong selection in China, and likewise, diversity increased in TaNAC020s during wheat polyploidization. The results obtained in this study demonstrate that TaNAC020s positively influence starch synthesis and accumulation and are one of the key regulators of the kernel (seed) size and kernel number and have the potential for utilization in wheat breeding to improve grain yield. Molecular markers developed in this study stand instrumental in marker-assisted selection for genetic improvement and germplasm enhancement in wheat.https://www.frontiersin.org/articles/10.3389/fgene.2022.956921/fullthousand kernel weightkernel lengthKASP markersTaNAC020marker–trait association |
| spellingShingle | Uzma Majeed Jian Hou Chenyang Hao Xueyong Zhang TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat Frontiers in Genetics thousand kernel weight kernel length KASP markers TaNAC020 marker–trait association |
| title | TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat |
| title_full | TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat |
| title_fullStr | TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat |
| title_full_unstemmed | TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat |
| title_short | TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat |
| title_sort | tanac020 homoeologous genes are associated with higher thousand kernel weight and kernel length in chinese wheat |
| topic | thousand kernel weight kernel length KASP markers TaNAC020 marker–trait association |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.956921/full |
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