Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach
Summary: Polypharmacology aids in the identification of multiple protein targets involved in disease pathology and selecting appropriate therapeutic compounds interacting with protein targets. Here, we present a protocol to identify the targets involved in obesity-linked diabetes and suitable phytoc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004070 |
| _version_ | 1797745128613347328 |
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| author | Akshaya Simha N Shashank M. Patil Akhila Chagalamari A.M. Satish Ramith Ramu |
| author_facet | Akshaya Simha N Shashank M. Patil Akhila Chagalamari A.M. Satish Ramith Ramu |
| author_sort | Akshaya Simha N |
| collection | DOAJ |
| description | Summary: Polypharmacology aids in the identification of multiple protein targets involved in disease pathology and selecting appropriate therapeutic compounds interacting with protein targets. Here, we present a protocol to identify the targets involved in obesity-linked diabetes and suitable phytocompounds to bind with the identified target. We describe steps to install and use softwares for identifying several protein targets by linking multiple diseases. This protocol allows the use of therapeutic compounds of both phytochemical and synthetic origins.For complete details on the use and execution of this protocol, please refer to Martiz et al.,1 and Maradesha et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T15:19:02Z |
| format | Article |
| id | doaj.art-8fc05ac35f624274a3575611b2e72397 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T15:19:02Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8fc05ac35f624274a3575611b2e723972023-08-11T05:35:20ZengElsevierSTAR Protocols2666-16672023-09-0143102440Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approachAkshaya Simha N0Shashank M. Patil1Akhila Chagalamari2A.M. Satish3Ramith Ramu4Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore 570015, Karnataka, IndiaDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore 570015, Karnataka, IndiaDepartment of Pharmacology, JSS Medical College, JSS Academy of Higher Education & Research, Mysore 570015, Karnataka, IndiaDepartment of Pharmacology, JSS Medical College, JSS Academy of Higher Education & Research, Mysore 570015, Karnataka, India; Corresponding authorDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore 570015, Karnataka, India; Corresponding authorSummary: Polypharmacology aids in the identification of multiple protein targets involved in disease pathology and selecting appropriate therapeutic compounds interacting with protein targets. Here, we present a protocol to identify the targets involved in obesity-linked diabetes and suitable phytocompounds to bind with the identified target. We describe steps to install and use softwares for identifying several protein targets by linking multiple diseases. This protocol allows the use of therapeutic compounds of both phytochemical and synthetic origins.For complete details on the use and execution of this protocol, please refer to Martiz et al.,1 and Maradesha et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004070BioinformaticsHigh Throughput ScreeningProtein BiochemistryStructural Biology |
| spellingShingle | Akshaya Simha N Shashank M. Patil Akhila Chagalamari A.M. Satish Ramith Ramu Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach STAR Protocols Bioinformatics High Throughput Screening Protein Biochemistry Structural Biology |
| title | Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach |
| title_full | Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach |
| title_fullStr | Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach |
| title_full_unstemmed | Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach |
| title_short | Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach |
| title_sort | protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach |
| topic | Bioinformatics High Throughput Screening Protein Biochemistry Structural Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004070 |
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