Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein
As the only major retrograde transporter along microtubules, cytoplasmic dynein plays crucial roles in the intracellular transport of organelles and other cargoes. Central to the function of this motor protein complex is dynein intermediate chain (IC), which binds the three dimeric dynein light chai...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/80217 |
| _version_ | 1797979678122704896 |
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| author | Kayla A Jara Nikolaus M Loening Patrick N Reardon Zhen Yu Prajna Woonnimani Coban Brooks Cat H Vesely Elisar J Barbar |
| author_facet | Kayla A Jara Nikolaus M Loening Patrick N Reardon Zhen Yu Prajna Woonnimani Coban Brooks Cat H Vesely Elisar J Barbar |
| author_sort | Kayla A Jara |
| collection | DOAJ |
| description | As the only major retrograde transporter along microtubules, cytoplasmic dynein plays crucial roles in the intracellular transport of organelles and other cargoes. Central to the function of this motor protein complex is dynein intermediate chain (IC), which binds the three dimeric dynein light chains at multivalent sites, and dynactin p150Glued and nuclear distribution protein (NudE) at overlapping sites of its intrinsically disordered N-terminal domain. The disorder in IC has hindered cryo-electron microscopy and X-ray crystallography studies of its structure and interactions. Here we use a suite of biophysical methods to reveal how multivalent binding of the three light chains regulates IC interactions with p150Glued and NudE. Using IC from Chaetomium thermophilum, a tractable species to interrogate IC interactions, we identify a significant reduction in binding affinity of IC to p150Glued and a loss of binding to NudE for constructs containing the entire N-terminal domain as well as for full-length constructs when compared to the tight binding observed with short IC constructs. We attribute this difference to autoinhibition caused by long-range intramolecular interactions between the N-terminal single α-helix of IC, the common site for p150Glued, and NudE binding, and residues closer to the end of the N-terminal domain. Reconstitution of IC subcomplexes demonstrates that autoinhibition is differentially regulated by light chains binding, underscoring their importance both in assembly and organization of IC, and in selection between multiple binding partners at the same site. |
| first_indexed | 2024-04-11T05:42:40Z |
| format | Article |
| id | doaj.art-8fc3ccdb65874b4c917186036545a17d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T05:42:40Z |
| publishDate | 2022-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8fc3ccdb65874b4c917186036545a17d2022-12-22T04:42:25ZengeLife Sciences Publications LtdeLife2050-084X2022-11-011110.7554/eLife.80217Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution proteinKayla A Jara0https://orcid.org/0000-0003-0406-2957Nikolaus M Loening1https://orcid.org/0000-0002-5074-6906Patrick N Reardon2Zhen Yu3Prajna Woonnimani4Coban Brooks5Cat H Vesely6Elisar J Barbar7https://orcid.org/0000-0003-4892-5259Department of Biochemistry and Biophysics, Oregon State University, Corvallis, United StatesDepartment of Chemistry, Lewis & Clark College, Portland, United StatesDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, United States; Oregon State University NMR Facility, Corvallis, United StatesDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, United StatesDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, United StatesDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, United StatesDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, United StatesDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, United StatesAs the only major retrograde transporter along microtubules, cytoplasmic dynein plays crucial roles in the intracellular transport of organelles and other cargoes. Central to the function of this motor protein complex is dynein intermediate chain (IC), which binds the three dimeric dynein light chains at multivalent sites, and dynactin p150Glued and nuclear distribution protein (NudE) at overlapping sites of its intrinsically disordered N-terminal domain. The disorder in IC has hindered cryo-electron microscopy and X-ray crystallography studies of its structure and interactions. Here we use a suite of biophysical methods to reveal how multivalent binding of the three light chains regulates IC interactions with p150Glued and NudE. Using IC from Chaetomium thermophilum, a tractable species to interrogate IC interactions, we identify a significant reduction in binding affinity of IC to p150Glued and a loss of binding to NudE for constructs containing the entire N-terminal domain as well as for full-length constructs when compared to the tight binding observed with short IC constructs. We attribute this difference to autoinhibition caused by long-range intramolecular interactions between the N-terminal single α-helix of IC, the common site for p150Glued, and NudE binding, and residues closer to the end of the N-terminal domain. Reconstitution of IC subcomplexes demonstrates that autoinhibition is differentially regulated by light chains binding, underscoring their importance both in assembly and organization of IC, and in selection between multiple binding partners at the same site.https://elifesciences.org/articles/80217dyneinp150 Gluedmultivalencyprotein disorderNMRprotein interactions |
| spellingShingle | Kayla A Jara Nikolaus M Loening Patrick N Reardon Zhen Yu Prajna Woonnimani Coban Brooks Cat H Vesely Elisar J Barbar Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein eLife dynein p150 Glued multivalency protein disorder NMR protein interactions |
| title | Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein |
| title_full | Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein |
| title_fullStr | Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein |
| title_full_unstemmed | Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein |
| title_short | Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein |
| title_sort | multivalency autoinhibition and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein |
| topic | dynein p150 Glued multivalency protein disorder NMR protein interactions |
| url | https://elifesciences.org/articles/80217 |
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