Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus
Jin Liang,1,* Yuanfang Hu,1,* Mingxia Fu,2 Na Li,1 Fengxia Wang,1 Xiaojun Yu,1 Bing Ji1 1Department of Laboratory Medicine, Binzhou Medical University Hospital, Binzhou, People’s Republic of China; 2Department of Laboratory Medicine, Binzhou People’s Hospital, Binzhou, People’s Repub...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-01-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/resistance-and-molecular-characteristics-of-methicillin-resistant-stap-peer-reviewed-fulltext-article-IDR |
| _version_ | 1797944633095880704 |
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| author | Liang J Hu Y Fu M Li N Wang F Yu X Ji B |
| author_facet | Liang J Hu Y Fu M Li N Wang F Yu X Ji B |
| author_sort | Liang J |
| collection | DOAJ |
| description | Jin Liang,1,* Yuanfang Hu,1,* Mingxia Fu,2 Na Li,1 Fengxia Wang,1 Xiaojun Yu,1 Bing Ji1 1Department of Laboratory Medicine, Binzhou Medical University Hospital, Binzhou, People’s Republic of China; 2Department of Laboratory Medicine, Binzhou People’s Hospital, Binzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing Ji, Email bingji1218@163.comObjective: To investigate the molecular epidemiology of Staphylococcus aureus (S. aureus) isolated from patients in the Yellow River Delta region and to construct antibiotic resistance profiles in different genetic backgrounds.Methods: Antibiotics susceptibility testing, staphylococcal protein A (spa) typing and accessory gene regulator (agr) typing were performed for all the 204 strains. Isolates with the positive mecA gene and heterogeneous vancomycin-intermediate S. aureus (hVISA) were subjected to multilocus sequence typing (MLST) and Staphylococcal chromosomal cassettes mec (SCCmec) typing.Results: Thirty-nine MRSA strains were identified by mecA gene. Twenty-two hVISA isolates including 9 MRSA and 13 methicillin-sensitive S. aureus (MSSA) strains were confirmed, the rest isolates (n = 182) were vancomycin-susceptible S. aureus (VSSA). 94.9% of MRSA and 63.6% of hVISA belonged to agrI. Fifty-seven distinct spa types including 5 novel types were mainly t309 (30.9%), t078 (11.8%) and t437 (11.8%). Fourteen sequence types (STs) containing 3 new STs were classified into 3 clone complexes (CCs) and 7 singletons among MRSA and hVISA isolates. Most MRSA isolates (87.2%) belonged to type Ⅳ SCCmec.Conclusion: The predominant genotype among MRSA population was ST59-t437-agrI-IVa (53.8%), followed by ST72-t2431-agrI-IVF (15.4%). ST72 and CC5 (ST5/965/7197) were the most common hVISA clones. Both CC59 (ST59/7437) and ST72 clones were resistant to erythromycin and clindamycin among MRSA population. Strains of MSSA with phenotypic hVISA (MS-hVISA) exhibited a striking genetic diversity accompanied by the diversification of drug resistance patterns.Keywords: Staphylococcus aureus, heterogeneous vancomycin-intermediateS. aureus, accessory gene regulator, multilocus sequence typing, erythromycin |
| first_indexed | 2024-04-10T20:42:51Z |
| format | Article |
| id | doaj.art-8fc509d145de4c5f848f0ff8d8e28300 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-10T20:42:51Z |
| publishDate | 2023-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-8fc509d145de4c5f848f0ff8d8e283002023-01-24T18:10:56ZengDove Medical PressInfection and Drug Resistance1178-69732023-01-01Volume 1637938881173Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureusLiang JHu YFu MLi NWang FYu XJi BJin Liang,1,* Yuanfang Hu,1,* Mingxia Fu,2 Na Li,1 Fengxia Wang,1 Xiaojun Yu,1 Bing Ji1 1Department of Laboratory Medicine, Binzhou Medical University Hospital, Binzhou, People’s Republic of China; 2Department of Laboratory Medicine, Binzhou People’s Hospital, Binzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing Ji, Email bingji1218@163.comObjective: To investigate the molecular epidemiology of Staphylococcus aureus (S. aureus) isolated from patients in the Yellow River Delta region and to construct antibiotic resistance profiles in different genetic backgrounds.Methods: Antibiotics susceptibility testing, staphylococcal protein A (spa) typing and accessory gene regulator (agr) typing were performed for all the 204 strains. Isolates with the positive mecA gene and heterogeneous vancomycin-intermediate S. aureus (hVISA) were subjected to multilocus sequence typing (MLST) and Staphylococcal chromosomal cassettes mec (SCCmec) typing.Results: Thirty-nine MRSA strains were identified by mecA gene. Twenty-two hVISA isolates including 9 MRSA and 13 methicillin-sensitive S. aureus (MSSA) strains were confirmed, the rest isolates (n = 182) were vancomycin-susceptible S. aureus (VSSA). 94.9% of MRSA and 63.6% of hVISA belonged to agrI. Fifty-seven distinct spa types including 5 novel types were mainly t309 (30.9%), t078 (11.8%) and t437 (11.8%). Fourteen sequence types (STs) containing 3 new STs were classified into 3 clone complexes (CCs) and 7 singletons among MRSA and hVISA isolates. Most MRSA isolates (87.2%) belonged to type Ⅳ SCCmec.Conclusion: The predominant genotype among MRSA population was ST59-t437-agrI-IVa (53.8%), followed by ST72-t2431-agrI-IVF (15.4%). ST72 and CC5 (ST5/965/7197) were the most common hVISA clones. Both CC59 (ST59/7437) and ST72 clones were resistant to erythromycin and clindamycin among MRSA population. Strains of MSSA with phenotypic hVISA (MS-hVISA) exhibited a striking genetic diversity accompanied by the diversification of drug resistance patterns.Keywords: Staphylococcus aureus, heterogeneous vancomycin-intermediateS. aureus, accessory gene regulator, multilocus sequence typing, erythromycinhttps://www.dovepress.com/resistance-and-molecular-characteristics-of-methicillin-resistant-stap-peer-reviewed-fulltext-article-IDRstaphylococcus aureusheterogeneous vancomycine-intermediate s. aureusaccessory gene regulatormultilocus sequence typingerythromycin |
| spellingShingle | Liang J Hu Y Fu M Li N Wang F Yu X Ji B Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Infection and Drug Resistance staphylococcus aureus heterogeneous vancomycine-intermediate s. aureus accessory gene regulator multilocus sequence typing erythromycin |
| title | Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus |
| title_full | Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus |
| title_fullStr | Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus |
| title_full_unstemmed | Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus |
| title_short | Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Heterogeneous Vancomycin-Intermediate Staphylococcus aureus |
| title_sort | resistance and molecular characteristics of methicillin resistant staphylococcus aureus and heterogeneous vancomycin intermediate staphylococcus aureus |
| topic | staphylococcus aureus heterogeneous vancomycine-intermediate s. aureus accessory gene regulator multilocus sequence typing erythromycin |
| url | https://www.dovepress.com/resistance-and-molecular-characteristics-of-methicillin-resistant-stap-peer-reviewed-fulltext-article-IDR |
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