New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
Wheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes...
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MDPI AG
2021-12-01
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| author | Chandan Roy Philomin Juliana Muhammad R. Kabir Krishna K. Roy Navin C. Gahtyari Felix Marza Xinyao He Gyanendra P. Singh Aakash Chawade Arun K. Joshi Pawan K. Singh |
| author_facet | Chandan Roy Philomin Juliana Muhammad R. Kabir Krishna K. Roy Navin C. Gahtyari Felix Marza Xinyao He Gyanendra P. Singh Aakash Chawade Arun K. Joshi Pawan K. Singh |
| author_sort | Chandan Roy |
| collection | DOAJ |
| description | Wheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes from India and Bangladesh was evaluated for wheat blast resistance and a genome-wide association study (GWAS) was performed. Disease evaluation was done during five crop seasons—at precision phenotyping platform (PPPs) for wheat blast disease at Jashore (2018–19), Quirusillas (2018–19 and 2019–20) and Okinawa (2019 and 2020). Single nucleotide polymorphisms (SNP) across the genome were obtained using DArTseq genotyping-by-sequencing platform, and in total 5713 filtered markers were used. GWAS revealed 40 significant markers associated with WB resistance, of which 33 (82.5%) were in the 2NS/2AS chromosome segment and one each on seven chromosomes (3B, 3D, 4A, 5A, 5D, 6A and 6B). The 2NS markers contributed significantly in most of the environments, explaining an average of 33.4% of the phenotypic variation. Overall, 22.4% of the germplasm carried 2NS/2AS segment. So far, 2NS translocation is the only effective WB resistance source being used in the breeding programs of South Asia. Nevertheless, the identification of non-2NS/2AS genomic regions for WB resistance provides a hope to broaden and diversify resistance for this disease in years to come. |
| first_indexed | 2024-03-10T03:16:39Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:16:39Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-8fc5865c41594901b79b75355cdafdf72023-11-23T10:11:50ZengMDPI AGPlants2223-77472021-12-011012269310.3390/plants10122693New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian GermplasmChandan Roy0Philomin Juliana1Muhammad R. Kabir2Krishna K. Roy3Navin C. Gahtyari4Felix Marza5Xinyao He6Gyanendra P. Singh7Aakash Chawade8Arun K. Joshi9Pawan K. Singh10Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour 813210, IndiaBISA/CIMMYT-India, NASC Complex, DPS Marg, New Delhi 110012, IndiaBangladesh Wheat and Maize Research Institute (BWMRI), Nashipur, Dinajpur 5200, BangladeshBangladesh Wheat and Maize Research Institute (BWMRI), Nashipur, Dinajpur 5200, BangladeshICAR–Vivekanand Parvatiya Krishi Anushandhan Sansthan, Almora 263601, IndiaInstituto Nacional de Innovación Agropecuaria y Forestal (INIAF), La Paz, BoliviaInternational Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, Mexico DF 06600, MexicoICAR—Indian Institute of Wheat and Barley Research, Karnal, Maharaja Agarsain Marg, P.O. Box 158, Karnal 132001, IndiaDepartment of Plant Breeding, Swedish University of Agricultural Sciences, 23053 Alnarp, SwedenBISA/CIMMYT-India, NASC Complex, DPS Marg, New Delhi 110012, IndiaInternational Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, Mexico DF 06600, MexicoWheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes from India and Bangladesh was evaluated for wheat blast resistance and a genome-wide association study (GWAS) was performed. Disease evaluation was done during five crop seasons—at precision phenotyping platform (PPPs) for wheat blast disease at Jashore (2018–19), Quirusillas (2018–19 and 2019–20) and Okinawa (2019 and 2020). Single nucleotide polymorphisms (SNP) across the genome were obtained using DArTseq genotyping-by-sequencing platform, and in total 5713 filtered markers were used. GWAS revealed 40 significant markers associated with WB resistance, of which 33 (82.5%) were in the 2NS/2AS chromosome segment and one each on seven chromosomes (3B, 3D, 4A, 5A, 5D, 6A and 6B). The 2NS markers contributed significantly in most of the environments, explaining an average of 33.4% of the phenotypic variation. Overall, 22.4% of the germplasm carried 2NS/2AS segment. So far, 2NS translocation is the only effective WB resistance source being used in the breeding programs of South Asia. Nevertheless, the identification of non-2NS/2AS genomic regions for WB resistance provides a hope to broaden and diversify resistance for this disease in years to come.https://www.mdpi.com/2223-7747/10/12/2693GWASSNPsgenotyping-by-sequencingwheat blast |
| spellingShingle | Chandan Roy Philomin Juliana Muhammad R. Kabir Krishna K. Roy Navin C. Gahtyari Felix Marza Xinyao He Gyanendra P. Singh Aakash Chawade Arun K. Joshi Pawan K. Singh New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm Plants GWAS SNPs genotyping-by-sequencing wheat blast |
| title | New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm |
| title_full | New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm |
| title_fullStr | New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm |
| title_full_unstemmed | New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm |
| title_short | New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm |
| title_sort | new genotypes and genomic regions for resistance to wheat blast in south asian germplasm |
| topic | GWAS SNPs genotyping-by-sequencing wheat blast |
| url | https://www.mdpi.com/2223-7747/10/12/2693 |
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