Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense
The lysine crotonylation of histone proteins is a newly identified posttranslational modification with diversified cellular functions. However, there are few reports on lysine crotonylation of non-histone proteins in medicinal plant cells. By using high-resolution liquid chromatography–mass spectrom...
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| Format: | Article |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.822374/full |
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| author | Jing Wu Jing Wu Xiaoxi Meng Weimin Jiang Zhaojian Wang Jing Zhang Fei Meng Xiaoyan Yao Mengjuan Ye Liang Yao Longhai Wang Nianjun Yu Nianjun Yu Daiyin Peng Daiyin Peng Daiyin Peng Shihai Xing Shihai Xing Shihai Xing |
| author_facet | Jing Wu Jing Wu Xiaoxi Meng Weimin Jiang Zhaojian Wang Jing Zhang Fei Meng Xiaoyan Yao Mengjuan Ye Liang Yao Longhai Wang Nianjun Yu Nianjun Yu Daiyin Peng Daiyin Peng Daiyin Peng Shihai Xing Shihai Xing Shihai Xing |
| author_sort | Jing Wu |
| collection | DOAJ |
| description | The lysine crotonylation of histone proteins is a newly identified posttranslational modification with diversified cellular functions. However, there are few reports on lysine crotonylation of non-histone proteins in medicinal plant cells. By using high-resolution liquid chromatography–mass spectrometry (LC-MS) coupled with highly sensitive-specific immune-affinity antibody analysis, a whole crotonylation proteome analysis of Dendrobium huoshanense was performed. In total, 1,591 proteins with 4,726 lysine crotonylation sites were identified; among them, 11 conserved motifs were identified. Bioinformatic analyses linked crotonylated proteins to the drought stress response and multiple metabolic pathways, including secondary metabolite biosynthesis, transport and catabolism, energy production and conversion, carbohydrate transport and metabolism, translation, and ribosomal structure and biogenesis. This study contributes toward understanding the regulatory mechanism of polysaccharide biosynthesis at the crotonylation level even under abiotic stress. |
| first_indexed | 2024-12-13T03:35:43Z |
| format | Article |
| id | doaj.art-8fc5f28f0a514e18bc240890facee58f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T03:35:43Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8fc5f28f0a514e18bc240890facee58f2022-12-22T00:01:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011310.3389/fpls.2022.822374822374Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanenseJing Wu0Jing Wu1Xiaoxi Meng2Weimin Jiang3Zhaojian Wang4Jing Zhang5Fei Meng6Xiaoyan Yao7Mengjuan Ye8Liang Yao9Longhai Wang10Nianjun Yu11Nianjun Yu12Daiyin Peng13Daiyin Peng14Daiyin Peng15Shihai Xing16Shihai Xing17Shihai Xing18College of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaInstitute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, ChinaDepartment of Horticultural Science, University of Minnesota, Saint Paul, MN, United StatesHunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaSchool of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaInstitute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaInstitute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, ChinaSynergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, ChinaCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaInstitute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, ChinaAnhui Province Key Laboratory of Research and Development of Chinese Medicine, Hefei, ChinaThe lysine crotonylation of histone proteins is a newly identified posttranslational modification with diversified cellular functions. However, there are few reports on lysine crotonylation of non-histone proteins in medicinal plant cells. By using high-resolution liquid chromatography–mass spectrometry (LC-MS) coupled with highly sensitive-specific immune-affinity antibody analysis, a whole crotonylation proteome analysis of Dendrobium huoshanense was performed. In total, 1,591 proteins with 4,726 lysine crotonylation sites were identified; among them, 11 conserved motifs were identified. Bioinformatic analyses linked crotonylated proteins to the drought stress response and multiple metabolic pathways, including secondary metabolite biosynthesis, transport and catabolism, energy production and conversion, carbohydrate transport and metabolism, translation, and ribosomal structure and biogenesis. This study contributes toward understanding the regulatory mechanism of polysaccharide biosynthesis at the crotonylation level even under abiotic stress.https://www.frontiersin.org/articles/10.3389/fpls.2022.822374/fullDendrobium huoshanenselysine crotonylationpolysaccharide biosynthesisheavily-crotonylatedphotosynthesis |
| spellingShingle | Jing Wu Jing Wu Xiaoxi Meng Weimin Jiang Zhaojian Wang Jing Zhang Fei Meng Xiaoyan Yao Mengjuan Ye Liang Yao Longhai Wang Nianjun Yu Nianjun Yu Daiyin Peng Daiyin Peng Daiyin Peng Shihai Xing Shihai Xing Shihai Xing Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense Frontiers in Plant Science Dendrobium huoshanense lysine crotonylation polysaccharide biosynthesis heavily-crotonylated photosynthesis |
| title | Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense |
| title_full | Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense |
| title_fullStr | Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense |
| title_full_unstemmed | Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense |
| title_short | Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense |
| title_sort | qualitative proteome wide analysis reveals the diverse functions of lysine crotonylation in dendrobium huoshanense |
| topic | Dendrobium huoshanense lysine crotonylation polysaccharide biosynthesis heavily-crotonylated photosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.822374/full |
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