Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells
Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis s...
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MDPI AG
2022-06-01
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| author | Ana Sofia Freitas Marta Costa Olívia Pontes Veronique Seidel Fernanda Proença Susana M. Cardoso Rui Oliveira Fátima Baltazar Cristina Almeida-Aguiar |
| author_facet | Ana Sofia Freitas Marta Costa Olívia Pontes Veronique Seidel Fernanda Proença Susana M. Cardoso Rui Oliveira Fátima Baltazar Cristina Almeida-Aguiar |
| author_sort | Ana Sofia Freitas |
| collection | DOAJ |
| description | Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment. |
| first_indexed | 2024-03-09T10:27:21Z |
| format | Article |
| id | doaj.art-8fc78a45871048508a7dbc6abed4ca8a |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T10:27:21Z |
| publishDate | 2022-06-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8fc78a45871048508a7dbc6abed4ca8a2023-12-01T21:35:48ZengMDPI AGMolecules1420-30492022-06-012713400110.3390/molecules27134001Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer CellsAna Sofia Freitas0Marta Costa1Olívia Pontes2Veronique Seidel3Fernanda Proença4Susana M. Cardoso5Rui Oliveira6Fátima Baltazar7Cristina Almeida-Aguiar8Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Department of Biology, University of Minho, 4710-057 Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus of Gualtar, 4710-057 Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus of Gualtar, 4710-057 Braga, PortugalNatural Products Research Laboratory, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKDepartment of Chemistry, University of Minho, Campus of Gualtar, 4710-057 Braga, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalDepartment of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus of Gualtar, 4710-057 Braga, PortugalDepartment of Biology, School of Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalRenal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (<i>n</i>-hexane, ethyl acetate, <i>n</i>-butanol and water) and three subfractions (<b>P1</b>–<b>P3</b>), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC<sub>50</sub> = 0.162 µg mL<sup>−1</sup>) and 786-O (IC<sub>50</sub> = 0.271 µg mL<sup>−1</sup>) cells. With similar toxicity against 786-O, <b>P1</b> (IC<sub>50</sub> = 3.8 µg mL<sup>−1</sup>) and <b>P3</b> (IC<sub>50</sub> = 3.1 µg mL<sup>−1</sup>) exhibited greater effect when combined (IC<sub>50</sub> = 2.5 µg mL<sup>−1</sup>). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.https://www.mdpi.com/1420-3049/27/13/4001propolisfractionationphenolic compoundspectolinarigenincytotoxic activityrenal cell carcinoma |
| spellingShingle | Ana Sofia Freitas Marta Costa Olívia Pontes Veronique Seidel Fernanda Proença Susana M. Cardoso Rui Oliveira Fátima Baltazar Cristina Almeida-Aguiar Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells Molecules propolis fractionation phenolic compounds pectolinarigenin cytotoxic activity renal cell carcinoma |
| title | Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells |
| title_full | Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells |
| title_fullStr | Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells |
| title_full_unstemmed | Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells |
| title_short | Selective Cytotoxicity of Portuguese Propolis Ethyl Acetate Fraction towards Renal Cancer Cells |
| title_sort | selective cytotoxicity of portuguese propolis ethyl acetate fraction towards renal cancer cells |
| topic | propolis fractionation phenolic compounds pectolinarigenin cytotoxic activity renal cell carcinoma |
| url | https://www.mdpi.com/1420-3049/27/13/4001 |
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