REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY)
Liver damages occur to 17.8% of blunt abdominal trauma cases. Postoperative mortality in liver damage is a high. It amounts to 4–10% in case of stab wounds, to 30.4–35,2% in case of closed injury, to 39.3% in case of concomitant injury. Complete hemostasis without damage to organ parenchyma is neede...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian State Medical University (Tomsk)
2014-12-01
|
| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/177 |
| _version_ | 1797872910310834176 |
|---|---|
| author | Ye. V. Semichev A. N. Baikov G. Ts. Dambayev P. S. Bushlanov A. N. Aleinik I. P. Polyakova Ye. O. Sharoglazova |
| author_facet | Ye. V. Semichev A. N. Baikov G. Ts. Dambayev P. S. Bushlanov A. N. Aleinik I. P. Polyakova Ye. O. Sharoglazova |
| author_sort | Ye. V. Semichev |
| collection | DOAJ |
| description | Liver damages occur to 17.8% of blunt abdominal trauma cases. Postoperative mortality in liver damage is a high. It amounts to 4–10% in case of stab wounds, to 30.4–35,2% in case of closed injury, to 39.3% in case of concomitant injury. Complete hemostasis without damage to organ parenchyma is needed for liver surgical operation.The aim of research is to evaluate the regenerative characteristics of liver tissue after hemostasis by nonequilibrium plasma in a long-term period.Material and methods. Research was performed on 20 laboratory rats, 3 experimental groups. 10 intact animals and 5 animals in groups on 90th and 180th day. Surgery is resection of liver left lobe and coagulation with cold plasma coagulator. The biochemical control (glucose, ALT, AST, total bilirubin, direct, alkaline phosphatase, total protein, urea, α-amylase, CRP, fibrinogen, aPTT, PTT, INR), morphological study of rat liver were performed.Results. After surgery with cold plasma hemostasis animals are active, early begin to eat. Fatal cases are not. Analysis of biochemical markers doesn't reveal statistically significant differences from the norm for the majority of indicators. Decrease of glucose concentration in the blood serum is observed in a long-term period. Histological analysis reveals an increase of the central and interlobular veins and moderate signs of edema on the 90th day. Hepatocytes with pronounced signs of protein and fatty degeneration are identified. Liver histology corresponds to the usual structure and is represented by liver lobules, separated by a small layer of connective tissue on the 180th day.Conclusion. Cold plasma coagulation in liver surgery showed effective hemostasis, minimal trauma of organ parenchyma, the lack of systemic effect and the subsequent complete regeneration of liver tissue in the treatment area. |
| first_indexed | 2024-04-10T01:07:40Z |
| format | Article |
| id | doaj.art-8fcb0644e1ea4f1491b7c30797882f93 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:07:40Z |
| publishDate | 2014-12-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-8fcb0644e1ea4f1491b7c30797882f932023-03-13T09:58:21ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842014-12-0113618018410.20538/1682-0363-2014-6-180-184175REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY)Ye. V. Semichev0A. N. Baikov1G. Ts. Dambayev2P. S. Bushlanov3A. N. Aleinik4I. P. Polyakova5Ye. O. Sharoglazova6Сибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскСибирский государственный медицинский университет, ТомскLiver damages occur to 17.8% of blunt abdominal trauma cases. Postoperative mortality in liver damage is a high. It amounts to 4–10% in case of stab wounds, to 30.4–35,2% in case of closed injury, to 39.3% in case of concomitant injury. Complete hemostasis without damage to organ parenchyma is needed for liver surgical operation.The aim of research is to evaluate the regenerative characteristics of liver tissue after hemostasis by nonequilibrium plasma in a long-term period.Material and methods. Research was performed on 20 laboratory rats, 3 experimental groups. 10 intact animals and 5 animals in groups on 90th and 180th day. Surgery is resection of liver left lobe and coagulation with cold plasma coagulator. The biochemical control (glucose, ALT, AST, total bilirubin, direct, alkaline phosphatase, total protein, urea, α-amylase, CRP, fibrinogen, aPTT, PTT, INR), morphological study of rat liver were performed.Results. After surgery with cold plasma hemostasis animals are active, early begin to eat. Fatal cases are not. Analysis of biochemical markers doesn't reveal statistically significant differences from the norm for the majority of indicators. Decrease of glucose concentration in the blood serum is observed in a long-term period. Histological analysis reveals an increase of the central and interlobular veins and moderate signs of edema on the 90th day. Hepatocytes with pronounced signs of protein and fatty degeneration are identified. Liver histology corresponds to the usual structure and is represented by liver lobules, separated by a small layer of connective tissue on the 180th day.Conclusion. Cold plasma coagulation in liver surgery showed effective hemostasis, minimal trauma of organ parenchyma, the lack of systemic effect and the subsequent complete regeneration of liver tissue in the treatment area.https://bulletin.ssmu.ru/jour/article/view/177холодноплазменная коагуляциякровотечение печенигемостаз паренхима-тозных кровотеченийатипичная резекция печенирегенерация |
| spellingShingle | Ye. V. Semichev A. N. Baikov G. Ts. Dambayev P. S. Bushlanov A. N. Aleinik I. P. Polyakova Ye. O. Sharoglazova REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY) Бюллетень сибирской медицины холодноплазменная коагуляция кровотечение печени гемостаз паренхима-тозных кровотечений атипичная резекция печени регенерация |
| title | REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY) |
| title_full | REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY) |
| title_fullStr | REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY) |
| title_full_unstemmed | REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY) |
| title_short | REGENERATIVE PECULIARITIES OF LIVER TISSUE AFTER HEMOSTASIS BY NONEQUILIBRIUM PLASMA ON 90TH AND 180TH DAY (EXPERIMENTAL STUDY) |
| title_sort | regenerative peculiarities of liver tissue after hemostasis by nonequilibrium plasma on 90th and 180th day experimental study |
| topic | холодноплазменная коагуляция кровотечение печени гемостаз паренхима-тозных кровотечений атипичная резекция печени регенерация |
| url | https://bulletin.ssmu.ru/jour/article/view/177 |
| work_keys_str_mv | AT yevsemichev regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy AT anbaikov regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy AT gtsdambayev regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy AT psbushlanov regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy AT analeinik regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy AT ippolyakova regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy AT yeosharoglazova regenerativepeculiaritiesoflivertissueafterhemostasisbynonequilibriumplasmaon90thand180thdayexperimentalstudy |