Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum
Recent studies support the hypothesis that microbes can seed some Alzheimer’s disease (AD) cases, leading to inflammation and overproduction of amyloid peptides. <i>Porphyromonas gingivalis</i> (Pg) is a keystone pathogen of chronic periodontitis and has been identified as risk factor fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/6/845 |
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| author | Raffaella Franciotti Pamela Pignatelli Claudia Carrarini Federica Maria Romei Martina Mastrippolito Antonella Gentile Rosa Mancinelli Stefania Fulle Adriano Piattelli Marco Onofrj Maria Cristina Curia |
| author_facet | Raffaella Franciotti Pamela Pignatelli Claudia Carrarini Federica Maria Romei Martina Mastrippolito Antonella Gentile Rosa Mancinelli Stefania Fulle Adriano Piattelli Marco Onofrj Maria Cristina Curia |
| author_sort | Raffaella Franciotti |
| collection | DOAJ |
| description | Recent studies support the hypothesis that microbes can seed some Alzheimer’s disease (AD) cases, leading to inflammation and overproduction of amyloid peptides. <i>Porphyromonas gingivalis</i> (Pg) is a keystone pathogen of chronic periodontitis and has been identified as risk factor for the development and progression of AD. The present preliminary study aimed to quantify Pg abundance in neurodegenerative disease (ND) patients compared with neurologic patients without neurodegenerative disorders (no-ND) and healthy controls (HC) to determine possible association between Pg abundance and neurodegenerative process. Pg was quantified on DNA extracted from the oral samples of 49 patients and 29 HC by quantitative polymerase chain reaction (qPCR). Anti-Pg antibodies were also detected on patient serum samples by enzyme-linked immunosorbent assays (ELISA). The Pg abundance in the oral cavity was significantly different among groups (<i>p</i> = 0.004). It was higher in ND than no-ND (<i>p</i> = 0.010) and HC (<i>p</i> = 0.008). The Pg abundance was correlated with the antibodies (<i>p</i> = 0.001) with different slopes between ND and no-ND (<i>p</i> = 0.037). Pg abundance was not correlated with oral indices and comorbidities. These results extend our understanding of the association between oral pathogens and AD to other neurodegenerative processes, confirming the hypothesis that oral pathogens can induce an antibody systemic response, influencing the progression of the disease. |
| first_indexed | 2024-03-10T10:41:10Z |
| format | Article |
| id | doaj.art-8fcb6ecc021744e898bea62f5cc46453 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T10:41:10Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-8fcb6ecc021744e898bea62f5cc464532023-11-21T22:58:42ZengMDPI AGBiomolecules2218-273X2021-06-0111684510.3390/biom11060845Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in SerumRaffaella Franciotti0Pamela Pignatelli1Claudia Carrarini2Federica Maria Romei3Martina Mastrippolito4Antonella Gentile5Rosa Mancinelli6Stefania Fulle7Adriano Piattelli8Marco Onofrj9Maria Cristina Curia10Department of Neuroscience, Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Neuroscience, Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Neuroscience, Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Neuroscience, Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Neuroscience, Imaging and Clinical Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66013 Chieti, ItalyRecent studies support the hypothesis that microbes can seed some Alzheimer’s disease (AD) cases, leading to inflammation and overproduction of amyloid peptides. <i>Porphyromonas gingivalis</i> (Pg) is a keystone pathogen of chronic periodontitis and has been identified as risk factor for the development and progression of AD. The present preliminary study aimed to quantify Pg abundance in neurodegenerative disease (ND) patients compared with neurologic patients without neurodegenerative disorders (no-ND) and healthy controls (HC) to determine possible association between Pg abundance and neurodegenerative process. Pg was quantified on DNA extracted from the oral samples of 49 patients and 29 HC by quantitative polymerase chain reaction (qPCR). Anti-Pg antibodies were also detected on patient serum samples by enzyme-linked immunosorbent assays (ELISA). The Pg abundance in the oral cavity was significantly different among groups (<i>p</i> = 0.004). It was higher in ND than no-ND (<i>p</i> = 0.010) and HC (<i>p</i> = 0.008). The Pg abundance was correlated with the antibodies (<i>p</i> = 0.001) with different slopes between ND and no-ND (<i>p</i> = 0.037). Pg abundance was not correlated with oral indices and comorbidities. These results extend our understanding of the association between oral pathogens and AD to other neurodegenerative processes, confirming the hypothesis that oral pathogens can induce an antibody systemic response, influencing the progression of the disease.https://www.mdpi.com/2218-273X/11/6/845oral bacteriaperiodontitis<i>Porphyromonas gingivalis</i>neurological diseaseneurodegenerative diseasegenomic DNA |
| spellingShingle | Raffaella Franciotti Pamela Pignatelli Claudia Carrarini Federica Maria Romei Martina Mastrippolito Antonella Gentile Rosa Mancinelli Stefania Fulle Adriano Piattelli Marco Onofrj Maria Cristina Curia Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum Biomolecules oral bacteria periodontitis <i>Porphyromonas gingivalis</i> neurological disease neurodegenerative disease genomic DNA |
| title | Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum |
| title_full | Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum |
| title_fullStr | Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum |
| title_full_unstemmed | Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum |
| title_short | Exploring the Connection between <i>Porphyromonas gingivalis</i> and Neurodegenerative Diseases: A Pilot Quantitative Study on the Bacterium Abundance in Oral Cavity and the Amount of Antibodies in Serum |
| title_sort | exploring the connection between i porphyromonas gingivalis i and neurodegenerative diseases a pilot quantitative study on the bacterium abundance in oral cavity and the amount of antibodies in serum |
| topic | oral bacteria periodontitis <i>Porphyromonas gingivalis</i> neurological disease neurodegenerative disease genomic DNA |
| url | https://www.mdpi.com/2218-273X/11/6/845 |
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