Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway
<p>Abstract</p> <p>Background</p> <p>The cellular activity of many factors and pathways is required to execute the complex replication cycle of the human immunodeficiency virus type 1 (HIV-1). To reveal these cellular components, several extensive RNAi screens have been...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-01-01
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| Series: | Retrovirology |
| Subjects: | |
| Online Access: | http://www.retrovirology.com/content/9/1/7 |
| _version_ | 1830213719176511488 |
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| author | Nachmias Dikla Sklan Ella H Ehrlich Marcelo Bacharach Eran |
| author_facet | Nachmias Dikla Sklan Ella H Ehrlich Marcelo Bacharach Eran |
| author_sort | Nachmias Dikla |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The cellular activity of many factors and pathways is required to execute the complex replication cycle of the human immunodeficiency virus type 1 (HIV-1). To reveal these cellular components, several extensive RNAi screens have been performed, listing numerous 'HIV-dependency factors'. However, only a small overlap between these lists exists, calling for further evaluation of the relevance of specific factors to HIV-1 replication and for the identification of additional cellular candidates. TBC1D20, the GTPase-activating protein (GAP) of Rab1, regulates endoplasmic reticulum (ER) to Golgi trafficking, was not identified in any of these screens, and its involvement in HIV-1 replication cycle is tested here.</p> <p>Findings</p> <p>Excessive TBC1D20 activity perturbs the early trafficking of HIV-1 envelope protein through the secretory pathway. Overexpression of TBC1D20 hampered envelope processing and reduced its association with detergent-resistant membranes, entailing a reduction in infectivity of HIV-1 virion like particles (VLPs).</p> <p>Conclusions</p> <p>These findings add TBC1D20 to the network of host factors regulating HIV replication cycle.</p> |
| first_indexed | 2024-12-18T06:29:53Z |
| format | Article |
| id | doaj.art-8fcc1b53188e4989bea18a05f5dfaaa9 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-18T06:29:53Z |
| publishDate | 2012-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-8fcc1b53188e4989bea18a05f5dfaaa92022-12-21T21:17:56ZengBMCRetrovirology1742-46902012-01-0191710.1186/1742-4690-9-7Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathwayNachmias DiklaSklan Ella HEhrlich MarceloBacharach Eran<p>Abstract</p> <p>Background</p> <p>The cellular activity of many factors and pathways is required to execute the complex replication cycle of the human immunodeficiency virus type 1 (HIV-1). To reveal these cellular components, several extensive RNAi screens have been performed, listing numerous 'HIV-dependency factors'. However, only a small overlap between these lists exists, calling for further evaluation of the relevance of specific factors to HIV-1 replication and for the identification of additional cellular candidates. TBC1D20, the GTPase-activating protein (GAP) of Rab1, regulates endoplasmic reticulum (ER) to Golgi trafficking, was not identified in any of these screens, and its involvement in HIV-1 replication cycle is tested here.</p> <p>Findings</p> <p>Excessive TBC1D20 activity perturbs the early trafficking of HIV-1 envelope protein through the secretory pathway. Overexpression of TBC1D20 hampered envelope processing and reduced its association with detergent-resistant membranes, entailing a reduction in infectivity of HIV-1 virion like particles (VLPs).</p> <p>Conclusions</p> <p>These findings add TBC1D20 to the network of host factors regulating HIV replication cycle.</p>http://www.retrovirology.com/content/9/1/7HIV-1envelopeassemblyTBCID20Rab1secretory pathway |
| spellingShingle | Nachmias Dikla Sklan Ella H Ehrlich Marcelo Bacharach Eran Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway Retrovirology HIV-1 envelope assembly TBCID20 Rab1 secretory pathway |
| title | Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway |
| title_full | Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway |
| title_fullStr | Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway |
| title_full_unstemmed | Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway |
| title_short | Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway |
| title_sort | human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a tbc1d20 rab1 regulated pathway |
| topic | HIV-1 envelope assembly TBCID20 Rab1 secretory pathway |
| url | http://www.retrovirology.com/content/9/1/7 |
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