<it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen
<p>Abstract</p> <p>Background</p> <p>To date, the function of many hypothetical membrane proteins of <it>Mycobacterium tuberculosis </it>is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-04-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/109 |
| _version_ | 1831762121337077760 |
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| author | Vanegas Magnolia Curtidor Hernando Ocampo Marisol Cifuentes Diana P Forero Martha Patarroyo Manuel E Patarroyo Manuel A |
| author_facet | Vanegas Magnolia Curtidor Hernando Ocampo Marisol Cifuentes Diana P Forero Martha Patarroyo Manuel E Patarroyo Manuel A |
| author_sort | Vanegas Magnolia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>To date, the function of many hypothetical membrane proteins of <it>Mycobacterium tuberculosis </it>is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, the biological activity of peptides derived from the hypothetical membrane protein Rv0679c of <it>M. tuberculosis </it>and their involvement in pathogen-host interactions was assessed. Transcription of the <it>Rv0679c </it>gene was studied in 26 <it>Mycobacterium </it>spp. Strains. Antibodies raised against putative B-cell epitopes of Rv0679c were used in Western blot and immunoelectron microscopy assays. Synthetic peptides spanning the entire length of the protein were tested for their ability to bind to A549 and U937 cells. High-activity binding peptides (HABPs) identified in Rv0679c were tested for their ability to inhibit mycobacterial invasion into cells.</p> <p>Results</p> <p>The gene encoding Rv0679c was detected in all strains of the <it>M. tuberculosis </it>complex (MTC), but was only transcribed in <it>M. tuberculosis </it>H37Rv, <it>M. tuberculosis </it>H37Ra and <it>M. africanum</it>. Anti-Rv0679c antibodies specifically recognized the protein in <it>M. tuberculosis </it>H37Rv sonicate and showed its localization on mycobacterial surface. Four HABPs inhibited invasion of <it>M. tuberculosis </it>to target cells by up to 75%.</p> <p>Conclusions</p> <p>The results indicate that Rv0679c HABPs and in particular HABP 30979 could be playing an important role during <it>M. tuberculosis </it>invasion of host cells, and therefore could be interesting research targets for studies aimed at developing strategies to control tuberculosis.</p> |
| first_indexed | 2024-12-22T05:01:19Z |
| format | Article |
| id | doaj.art-8fcc33d0d74c451d897790858142e346 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-22T05:01:19Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-8fcc33d0d74c451d897790858142e3462022-12-21T18:38:16ZengBMCBMC Microbiology1471-21802010-04-0110110910.1186/1471-2180-10-109<it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigenVanegas MagnoliaCurtidor HernandoOcampo MarisolCifuentes Diana PForero MarthaPatarroyo Manuel EPatarroyo Manuel A<p>Abstract</p> <p>Background</p> <p>To date, the function of many hypothetical membrane proteins of <it>Mycobacterium tuberculosis </it>is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, the biological activity of peptides derived from the hypothetical membrane protein Rv0679c of <it>M. tuberculosis </it>and their involvement in pathogen-host interactions was assessed. Transcription of the <it>Rv0679c </it>gene was studied in 26 <it>Mycobacterium </it>spp. Strains. Antibodies raised against putative B-cell epitopes of Rv0679c were used in Western blot and immunoelectron microscopy assays. Synthetic peptides spanning the entire length of the protein were tested for their ability to bind to A549 and U937 cells. High-activity binding peptides (HABPs) identified in Rv0679c were tested for their ability to inhibit mycobacterial invasion into cells.</p> <p>Results</p> <p>The gene encoding Rv0679c was detected in all strains of the <it>M. tuberculosis </it>complex (MTC), but was only transcribed in <it>M. tuberculosis </it>H37Rv, <it>M. tuberculosis </it>H37Ra and <it>M. africanum</it>. Anti-Rv0679c antibodies specifically recognized the protein in <it>M. tuberculosis </it>H37Rv sonicate and showed its localization on mycobacterial surface. Four HABPs inhibited invasion of <it>M. tuberculosis </it>to target cells by up to 75%.</p> <p>Conclusions</p> <p>The results indicate that Rv0679c HABPs and in particular HABP 30979 could be playing an important role during <it>M. tuberculosis </it>invasion of host cells, and therefore could be interesting research targets for studies aimed at developing strategies to control tuberculosis.</p>http://www.biomedcentral.com/1471-2180/10/109 |
| spellingShingle | Vanegas Magnolia Curtidor Hernando Ocampo Marisol Cifuentes Diana P Forero Martha Patarroyo Manuel E Patarroyo Manuel A <it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen BMC Microbiology |
| title | <it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen |
| title_full | <it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen |
| title_fullStr | <it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen |
| title_full_unstemmed | <it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen |
| title_short | <it>Mycobacterium tuberculosis </it>Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen |
| title_sort | it mycobacterium tuberculosis it rv0679c protein sequences involved in host cell infection potential tb vaccine candidate antigen |
| url | http://www.biomedcentral.com/1471-2180/10/109 |
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