Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography
Summary: In traditional optical imaging, limited light penetration constrains high-resolution interrogation to tissue surfaces. Optoacoustic imaging combines the superb contrast of optical imaging with deep penetration of ultrasound, enabling a range of new applications. We used multispectral optoac...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-03-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719301822 |
| _version_ | 1818042919165100032 |
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| author | Ivan Olefir Ara Ghazaryan Hong Yang Jaber Malekzadeh-Najafabadi Sarah Glasl Panagiotis Symvoulidis Valerie B. O’Leary George Sergiadis Vasilis Ntziachristos Saak V. Ovsepian |
| author_facet | Ivan Olefir Ara Ghazaryan Hong Yang Jaber Malekzadeh-Najafabadi Sarah Glasl Panagiotis Symvoulidis Valerie B. O’Leary George Sergiadis Vasilis Ntziachristos Saak V. Ovsepian |
| author_sort | Ivan Olefir |
| collection | DOAJ |
| description | Summary: In traditional optical imaging, limited light penetration constrains high-resolution interrogation to tissue surfaces. Optoacoustic imaging combines the superb contrast of optical imaging with deep penetration of ultrasound, enabling a range of new applications. We used multispectral optoacoustic tomography (MSOT) for functional and structural neuroimaging in mice at resolution, depth, and specificity unattainable by other neuroimaging modalities. Based on multispectral readouts, we computed hemoglobin gradient and oxygen saturation changes related to processing of somatosensory signals in different structures along the entire subcortical-cortical axis. Using temporal correlation analysis and seed-based maps, we reveal the connectivity between cortical, thalamic, and sub-thalamic formations. With the same modality, high-resolution structural tomography of intact mouse brain was achieved based on endogenous contrasts, demonstrating near-perfect matches with anatomical features revealed by histology. These results extend the limits of noninvasive observations beyond the reach of standard high-resolution neuroimaging, verifying the suitability of MSOT for small-animal studies. : Olefir et al. apply multispectral optoacoustic (photoacoustic) tomography (MSOT) for noninvasive spatial and spectral mapping and decomposition of neural dynamics and organization of the intact mouse brain in vivo. The results extend the boundaries of noninvasive high-resolution observations beyond the reach of intravital optical neuroimaging in small-animal studies. Keywords: photoacoustic imaging, hemodynamic response, whole-brain tomography, near-infrared neuroimaging, label-free interrogation, temporal coherence |
| first_indexed | 2024-12-10T08:53:58Z |
| format | Article |
| id | doaj.art-8fcc5a19bfc9403fadcd5da193685bef |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-10T08:53:58Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-8fcc5a19bfc9403fadcd5da193685bef2022-12-22T01:55:29ZengElsevierCell Reports2211-12472019-03-01261028332846.e3Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic TomographyIvan Olefir0Ara Ghazaryan1Hong Yang2Jaber Malekzadeh-Najafabadi3Sarah Glasl4Panagiotis Symvoulidis5Valerie B. O’Leary6George Sergiadis7Vasilis Ntziachristos8Saak V. Ovsepian9Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, GermanyInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, GermanyInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, GermanyInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, GermanyInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, GermanyInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, GermanyDepartment of Medical Genetics, Third Faculty of Medicine of Charles University, 11636 Prague, Czech RepublicDepartment of Electrical and Computer Engineering, Aristotle University, 54124 Thessaloniki, GreeceInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, Germany; Corresponding authorInstitute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, Germany; Department of Experimental Neurobiology, National Institute of Mental Health, Topolová 748, 25067 Klecany, Czech Republic; Department of Psychiatry and Medical Psychology, Third Faculty of Medicine of Charles University, 11636 Prague, Czech Republic; Corresponding authorSummary: In traditional optical imaging, limited light penetration constrains high-resolution interrogation to tissue surfaces. Optoacoustic imaging combines the superb contrast of optical imaging with deep penetration of ultrasound, enabling a range of new applications. We used multispectral optoacoustic tomography (MSOT) for functional and structural neuroimaging in mice at resolution, depth, and specificity unattainable by other neuroimaging modalities. Based on multispectral readouts, we computed hemoglobin gradient and oxygen saturation changes related to processing of somatosensory signals in different structures along the entire subcortical-cortical axis. Using temporal correlation analysis and seed-based maps, we reveal the connectivity between cortical, thalamic, and sub-thalamic formations. With the same modality, high-resolution structural tomography of intact mouse brain was achieved based on endogenous contrasts, demonstrating near-perfect matches with anatomical features revealed by histology. These results extend the limits of noninvasive observations beyond the reach of standard high-resolution neuroimaging, verifying the suitability of MSOT for small-animal studies. : Olefir et al. apply multispectral optoacoustic (photoacoustic) tomography (MSOT) for noninvasive spatial and spectral mapping and decomposition of neural dynamics and organization of the intact mouse brain in vivo. The results extend the boundaries of noninvasive high-resolution observations beyond the reach of intravital optical neuroimaging in small-animal studies. Keywords: photoacoustic imaging, hemodynamic response, whole-brain tomography, near-infrared neuroimaging, label-free interrogation, temporal coherencehttp://www.sciencedirect.com/science/article/pii/S2211124719301822 |
| spellingShingle | Ivan Olefir Ara Ghazaryan Hong Yang Jaber Malekzadeh-Najafabadi Sarah Glasl Panagiotis Symvoulidis Valerie B. O’Leary George Sergiadis Vasilis Ntziachristos Saak V. Ovsepian Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography Cell Reports |
| title | Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography |
| title_full | Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography |
| title_fullStr | Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography |
| title_full_unstemmed | Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography |
| title_short | Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography |
| title_sort | spatial and spectral mapping and decomposition of neural dynamics and organization of the mouse brain with multispectral optoacoustic tomography |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719301822 |
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