Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)
Abstract Background The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), overruns date palm ranches in most Oases of Saudi Arabia and causes massive economic losses. Integrated pest management (IPM) of the RPW by biocontrol agents of bacterial isolates was the primary ai...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-05-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-022-00551-5 |
| _version_ | 1818005871542665216 |
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| author | Najeeb M. Almasoudi Khalid A. Asiry Kamal A. M. Abo-Elyousr |
| author_facet | Najeeb M. Almasoudi Khalid A. Asiry Kamal A. M. Abo-Elyousr |
| author_sort | Najeeb M. Almasoudi |
| collection | DOAJ |
| description | Abstract Background The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), overruns date palm ranches in most Oases of Saudi Arabia and causes massive economic losses. Integrated pest management (IPM) of the RPW by biocontrol agents of bacterial isolates was the primary aim of the present study. Results Thirty-seven bacterial isolates were isolated from the larvae of R. ferrugineus, which were collected from infested Phoenix dactylifera trees growing in different parts of Saudi Arabia. 16S rDNA sequencing showed that the isolated bacteria were: Serratia marcescens , Klebsiella pneumoniae and Bacillus thuringiensis. The potential of these isolates was tested against R. ferrugineus in the laboratory. The use of these three isolates showed significant mortality percentages against R. ferrugineus. The highest reduction in mortality was recorded at the concentration of 1 × 108 CFU/ml; B. thuringiensis isolate displayed the highest potency in mortal percentage rate (100%), 4 days post-treatment, followed by S. marcescens isolate after 5 days, and then K. pneumoniae isolate after 6 days. Conclusions These results suggest that the use of these bacterial isolates was very useful in in vitro experiments, and it may consider those bacterial strains of interest as a potential biocontrol agent of R. ferrugineus. |
| first_indexed | 2024-04-14T04:51:11Z |
| format | Article |
| id | doaj.art-8fccc0360a48476eb3e4d8c4a4595910 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-04-14T04:51:11Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-8fccc0360a48476eb3e4d8c4a45959102022-12-22T02:11:17ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422022-05-013211910.1186/s41938-022-00551-5Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)Najeeb M. Almasoudi0Khalid A. Asiry1Kamal A. M. Abo-Elyousr2Department of Arid Land Agriculture, King Abdulaziz UniversityDepartment of Arid Land Agriculture, King Abdulaziz UniversityDepartment of Arid Land Agriculture, King Abdulaziz UniversityAbstract Background The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), overruns date palm ranches in most Oases of Saudi Arabia and causes massive economic losses. Integrated pest management (IPM) of the RPW by biocontrol agents of bacterial isolates was the primary aim of the present study. Results Thirty-seven bacterial isolates were isolated from the larvae of R. ferrugineus, which were collected from infested Phoenix dactylifera trees growing in different parts of Saudi Arabia. 16S rDNA sequencing showed that the isolated bacteria were: Serratia marcescens , Klebsiella pneumoniae and Bacillus thuringiensis. The potential of these isolates was tested against R. ferrugineus in the laboratory. The use of these three isolates showed significant mortality percentages against R. ferrugineus. The highest reduction in mortality was recorded at the concentration of 1 × 108 CFU/ml; B. thuringiensis isolate displayed the highest potency in mortal percentage rate (100%), 4 days post-treatment, followed by S. marcescens isolate after 5 days, and then K. pneumoniae isolate after 6 days. Conclusions These results suggest that the use of these bacterial isolates was very useful in in vitro experiments, and it may consider those bacterial strains of interest as a potential biocontrol agent of R. ferrugineus.https://doi.org/10.1186/s41938-022-00551-5Biological controlRed palm weevilEntomopathogenicIntegrated pest management (IMP) |
| spellingShingle | Najeeb M. Almasoudi Khalid A. Asiry Kamal A. M. Abo-Elyousr Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) Egyptian Journal of Biological Pest Control Biological control Red palm weevil Entomopathogenic Integrated pest management (IMP) |
| title | Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) |
| title_full | Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) |
| title_fullStr | Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) |
| title_full_unstemmed | Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) |
| title_short | Isolation, identification and efficacy of three bacterial isolates against the red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) |
| title_sort | isolation identification and efficacy of three bacterial isolates against the red palm weevil rhynchophorus ferrugineus olivier coleoptera curculionidae |
| topic | Biological control Red palm weevil Entomopathogenic Integrated pest management (IMP) |
| url | https://doi.org/10.1186/s41938-022-00551-5 |
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