Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling.
Studying plant-aphid interactions is challenging as aphid feeding is a complex process hidden in the plant tissue. Here we propose a combination of two well established methods to study nutrient acquisition by aphids focusing on the uptake of isotopically labelled nitrogen ((15)N). We combined the E...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3871520?pdf=render |
| _version_ | 1828386685637885952 |
|---|---|
| author | Franziska Kuhlmann Sebastian E W Opitz Erich Inselsbacher Ulrika Ganeteg Torgny Näsholm Velemir Ninkovic |
| author_facet | Franziska Kuhlmann Sebastian E W Opitz Erich Inselsbacher Ulrika Ganeteg Torgny Näsholm Velemir Ninkovic |
| author_sort | Franziska Kuhlmann |
| collection | DOAJ |
| description | Studying plant-aphid interactions is challenging as aphid feeding is a complex process hidden in the plant tissue. Here we propose a combination of two well established methods to study nutrient acquisition by aphids focusing on the uptake of isotopically labelled nitrogen ((15)N). We combined the Electrical Penetration Graph (EPG) technique that allows detailed recording of aphid feeding behaviour and stable isotope ratio mass spectrometry (IRMS) to precisely measure the uptake of nitrogen. Bird cherry-oat aphids Rhopalosiphum padi L. (Hemiptera, Aphididae) fed for 24 h on barley plants (Hordeum vulgare L., cultivar Lina, Poaceae) that were cultivated with a (15)N enriched nutrient solution. The time aphids fed in the phloem was strongly positive correlated with their (15)N uptake. All other single behavioural phases were not correlated with (15)N enrichment in the aphids, which corroborates their classification as non-feeding EPG phases. In addition, phloem-feeding and (15)N enrichment of aphids was divided into two groups. One group spent only short time in the phloem phase and was unsuccessful in nitrogen acquisition, while the other group displayed longer phloem-feeding phases and was successful in nitrogen acquisition. This suggests that several factors such as the right feeding site, time span of feeding and individual conditions play a role for the aphids to acquire nutrients successfully. The power of this combination of methods for studying plant-aphid interactions is discussed. |
| first_indexed | 2024-12-10T05:41:14Z |
| format | Article |
| id | doaj.art-8fce35ff894e41a6abb90b308e4c988c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:41:14Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8fce35ff894e41a6abb90b308e4c988c2022-12-22T02:00:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8308510.1371/journal.pone.0083085Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling.Franziska KuhlmannSebastian E W OpitzErich InselsbacherUlrika GanetegTorgny NäsholmVelemir NinkovicStudying plant-aphid interactions is challenging as aphid feeding is a complex process hidden in the plant tissue. Here we propose a combination of two well established methods to study nutrient acquisition by aphids focusing on the uptake of isotopically labelled nitrogen ((15)N). We combined the Electrical Penetration Graph (EPG) technique that allows detailed recording of aphid feeding behaviour and stable isotope ratio mass spectrometry (IRMS) to precisely measure the uptake of nitrogen. Bird cherry-oat aphids Rhopalosiphum padi L. (Hemiptera, Aphididae) fed for 24 h on barley plants (Hordeum vulgare L., cultivar Lina, Poaceae) that were cultivated with a (15)N enriched nutrient solution. The time aphids fed in the phloem was strongly positive correlated with their (15)N uptake. All other single behavioural phases were not correlated with (15)N enrichment in the aphids, which corroborates their classification as non-feeding EPG phases. In addition, phloem-feeding and (15)N enrichment of aphids was divided into two groups. One group spent only short time in the phloem phase and was unsuccessful in nitrogen acquisition, while the other group displayed longer phloem-feeding phases and was successful in nitrogen acquisition. This suggests that several factors such as the right feeding site, time span of feeding and individual conditions play a role for the aphids to acquire nutrients successfully. The power of this combination of methods for studying plant-aphid interactions is discussed.http://europepmc.org/articles/PMC3871520?pdf=render |
| spellingShingle | Franziska Kuhlmann Sebastian E W Opitz Erich Inselsbacher Ulrika Ganeteg Torgny Näsholm Velemir Ninkovic Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling. PLoS ONE |
| title | Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling. |
| title_full | Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling. |
| title_fullStr | Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling. |
| title_full_unstemmed | Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling. |
| title_short | Exploring the nitrogen ingestion of aphids--a new method using electrical penetration graph and (15)N labelling. |
| title_sort | exploring the nitrogen ingestion of aphids a new method using electrical penetration graph and 15 n labelling |
| url | http://europepmc.org/articles/PMC3871520?pdf=render |
| work_keys_str_mv | AT franziskakuhlmann exploringthenitrogeningestionofaphidsanewmethodusingelectricalpenetrationgraphand15nlabelling AT sebastianewopitz exploringthenitrogeningestionofaphidsanewmethodusingelectricalpenetrationgraphand15nlabelling AT erichinselsbacher exploringthenitrogeningestionofaphidsanewmethodusingelectricalpenetrationgraphand15nlabelling AT ulrikaganeteg exploringthenitrogeningestionofaphidsanewmethodusingelectricalpenetrationgraphand15nlabelling AT torgnynasholm exploringthenitrogeningestionofaphidsanewmethodusingelectricalpenetrationgraphand15nlabelling AT velemirninkovic exploringthenitrogeningestionofaphidsanewmethodusingelectricalpenetrationgraphand15nlabelling |