Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants
Despite advances in control via snail eradication and large-scale chemotherapy using praziquental, schistosomiasis continues to spread to new geographic areas particularly in sub-Saharan Africa. Presently, there is no vaccine for controlling this disease. We have concentrated on a functionally impor...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2005-03-01
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| Series: | Parasite |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/parasite/2005121003 |
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| author | Siddiqui A.A. Pinkston J.R. Quinlin M.L. Kavikondala V. Rewers-Felkins K.A. Phillips T. Pompa J. |
| author_facet | Siddiqui A.A. Pinkston J.R. Quinlin M.L. Kavikondala V. Rewers-Felkins K.A. Phillips T. Pompa J. |
| author_sort | Siddiqui A.A. |
| collection | DOAJ |
| description | Despite advances in control via snail eradication and large-scale chemotherapy using praziquental, schistosomiasis continues to spread to new geographic areas particularly in sub-Saharan Africa. Presently, there is no vaccine for controlling this disease. We have concentrated on a functionally important schistosome antigen Sm-p80 as a possible vaccine candidate for schistosomiasis. Here we report the proliferation of spleen cells in response to the recombinant Sm-p80 protein and cytokine (IFN-γ and IL-4) production by the splenocytes. These spleen cells were obtained from groups of mice that were vaccinated with a DNA vaccine formulation containing Sm-p80 and one of the Th-1 (IL-2 or IL-12) or Th-2 (GM-CSF, IL-4) enhancer cytokines. The splenocytes from the groups of mice vaccinated with Sm-p80 DNA in the presence of Th-2 enhancer cytokines showed moderate but detectable proliferation. The splenocytes obtained from mice vaccinated with Sm-p80 DNA with Th-1 enhancer cytokines IL-2 and IL-12 provided the highest proliferation. The IFN-γ production by splenocytes was found to follow the similar pattern [(Sm-p80) < (Sm-p80 + IL-4) < (Sm-p80 + GM-CSF) < (Sm-p80 + IL-12) < (Sm-p80 + IL-2)], as has been observed for the proliferation and protection data. However, the elevated IL-4 production was inversely correlated to Sm-p80-induced splenocyte proliferation or the protection. These results show again that protective immune response induced by Sm-p80 is of Th-1 type. |
| first_indexed | 2024-03-09T09:49:05Z |
| format | Article |
| id | doaj.art-8fcf12ce79e84a6a9467ee823bb56ac9 |
| institution | Directory Open Access Journal |
| issn | 1252-607X 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T09:49:05Z |
| publishDate | 2005-03-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-8fcf12ce79e84a6a9467ee823bb56ac92023-12-02T00:19:28ZengEDP SciencesParasite1252-607X1776-10422005-03-011213810.1051/parasite/2005121003parasite2005121p3Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvantsSiddiqui A.A.Pinkston J.R.Quinlin M.L.Kavikondala V.Rewers-Felkins K.A.Phillips T.Pompa J.Despite advances in control via snail eradication and large-scale chemotherapy using praziquental, schistosomiasis continues to spread to new geographic areas particularly in sub-Saharan Africa. Presently, there is no vaccine for controlling this disease. We have concentrated on a functionally important schistosome antigen Sm-p80 as a possible vaccine candidate for schistosomiasis. Here we report the proliferation of spleen cells in response to the recombinant Sm-p80 protein and cytokine (IFN-γ and IL-4) production by the splenocytes. These spleen cells were obtained from groups of mice that were vaccinated with a DNA vaccine formulation containing Sm-p80 and one of the Th-1 (IL-2 or IL-12) or Th-2 (GM-CSF, IL-4) enhancer cytokines. The splenocytes from the groups of mice vaccinated with Sm-p80 DNA in the presence of Th-2 enhancer cytokines showed moderate but detectable proliferation. The splenocytes obtained from mice vaccinated with Sm-p80 DNA with Th-1 enhancer cytokines IL-2 and IL-12 provided the highest proliferation. The IFN-γ production by splenocytes was found to follow the similar pattern [(Sm-p80) < (Sm-p80 + IL-4) < (Sm-p80 + GM-CSF) < (Sm-p80 + IL-12) < (Sm-p80 + IL-2)], as has been observed for the proliferation and protection data. However, the elevated IL-4 production was inversely correlated to Sm-p80-induced splenocyte proliferation or the protection. These results show again that protective immune response induced by Sm-p80 is of Th-1 type.http://dx.doi.org/10.1051/parasite/2005121003schistosomiasisvaccineSm-p80 protein |
| spellingShingle | Siddiqui A.A. Pinkston J.R. Quinlin M.L. Kavikondala V. Rewers-Felkins K.A. Phillips T. Pompa J. Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants Parasite schistosomiasis vaccine Sm-p80 protein |
| title | Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants |
| title_full | Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants |
| title_fullStr | Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants |
| title_full_unstemmed | Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants |
| title_short | Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants |
| title_sort | characterization of protective immunity induced against schistosoma mansoni via dna priming with the large subunit of calpain sm p80 in the presence of genetic adjuvants |
| topic | schistosomiasis vaccine Sm-p80 protein |
| url | http://dx.doi.org/10.1051/parasite/2005121003 |
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